Twenty Traditional Algerian Plants Used in Diabetes Therapy as Strong Inhibitors of <i>α</i>-Amylase Activity

Khacheba, Ihcen; Djeridane, Amar; Yousfi, Mohamed

doi:10.1155/2014/287281

Cited by 21 publications

(6 citation statements)

References 28 publications

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“…It was found that TPC values were 108.6, 137.16, and 143.26 mg GAE/g and TFC values were 15.62, 21.96, and 27.63 mg QE/g for aqueous, hydro-alcoholic, and methanol extract, respectively [24]. Meanwhile, Khacheba et al [40] working on OA collected from Laghou at town, in the steppe region of Algeria, demonstrate that leaves (15.86 ± 0.02 and 6.54 ± 0.03mg RE/g DW) aqueous extracts are richer in polyphenols and flavonoids than the pods (9.17 ± 0.04 and 3.61 ± 0.03 mg GAE/g DW). In comparison with our results, it can be noticed that polyphenols content not only depends on the solvent polarity, but also on geographical localities, harvest season, as well as plant age.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Constituents and Antioxidant Activity of Oudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage

Hajlaoui

Arraouadi²,

Mighri

et al. 2019

Antioxidants

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Five Oudneya Africana (OA) leaves extracts were screened for their total phenolic (TPC), total flavonoid (TFC), condensed tannins (CTC) content, as well as their antioxidant capacity. The highest amount of TPC (661.66 ± 0.08 mg GAE/g), TFC (344.68 ± 0.44 mg QE/g) and TCT (90.18 ± 0.49 mg CE/g) was recorded to ethanol, acetone, and dichloromethane extracts, respectively. For 2,2-diphenyl-1-picrylhydrazyl (DPPH) (22.00 ± 0.03 µg/mL) and Reducing Power Assay (FRAP) (269.00 ± 0.01µg/mL) assays, ethanol extract showed the potent activity, while with ABTS test, acetone extract was the most active (761.15 ± 0.09 µg/mL). HPLC-MS analysis of acetonic and ethanolic extracts reveals the predominance of quinic acid, chlorogenic acid, 4-O-caffeoylquinic acid, and rutin compounds. The addition effect evaluation of OA extracts in beef burger preservation demonstrates the powerful effect (p < 0.05) of acetonic and ethanolic ones (0.03%) to inhibit lipids oxidation during storage for 10 days, given by the lowest increase in Thiobarbituric Acid-reactive Substances (TBARS) values as compared to the (−) control with a significant difference between free thiols values. In addition, these two extracts appear to be effective (p < 0.05) for pH stability, color, and sensory parameters as compared to (+) and (−) controls and aqueous extract. Hamburger odour was considered as a dependent variable in multiple linear regression analysis, where the models results showed that physicochemical parameters determine more burger odour than sensorial ones.

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Section: Resultsmentioning

confidence: 99%

Phytochemical Constituents and Antioxidant Activity of Oudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage

Hajlaoui

Arraouadi²,

Mighri

et al. 2019

Antioxidants

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“…In vitro -alpha amylase inhibitory activity analysis Decreasing post-prandial blood glucose level is one of the therapeutic approaches used to treat the Diabetes mellitus (Khacheba et al, 2014). This can be achieved by using some starch or carbohydrate blockers that delay the process of carbohydrate hydrolysis and absorption and thereby decreasing the postprandial increase of blood glucose after, a starch or mixed carbohydrate diet (Sales et al, 2011;McEwan et al., 2010).…”

Section: Resultsmentioning

confidence: 99%

In silico and in vitro assessment on antidiabetic efficacy of secondary metabolites from Syzygium cumini (L.) Skeels

Bansode¹,

Gupta

Salalkar³

2016

Plant Sci. Today

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India ranks high for prevalence of diabetes and the treatment of diabetes without any side effects is still challenging. Though herbal remedies help reduce the side effect, proper standardization of phytochemical which prove as a bioactive compound, its proper dose and clinical trials are lacking. In our investigation, we studied the binding mechanism of the secondary metabolites of Syzygium cumini, their in vitro antidiabetic activity and the number of phytochemicals present. In silico study revealed that ellagic acid has a potential to modulate the carbohydrate metabolizing enzyme activity showing higher affinity for the enzymes with much lesser binding energy, -4.73 kcal/mol for alpha amylase, -4.87 kcal/mol for beta-glucosidase, -4.79 kcal/mol for glycogen synthase kinase, -4.18 kcal/mol for glucokinase and -4.49 kcal/mol for alpha-glucosidase. In vitro-Alpha amylase inhibitory activity assay showed that ethanol extract has the highest value of percent inhibition (73.33%) as compared to standard drug Acarbose (65.99%). Finally, TLC analysis cleared that ethanol extract contains five compounds one of which may be a bioactive compound, ellagic acid. Further purification and characterization of the ellagic acid is needed.

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“…After incubating the mixture at 25 °C for 15 minutes, the enzyme reaction was complete. Lineweaver-Burk plot analysis of data from enzyme tests comprising various dilutions of the substrate and inhibitors according to Michaelis-Menten kinetics was used to establish the manner of inhibition for these inhibitors (22,23) .…”

Section: Kinetic Parametersmentioning

confidence: 99%

Inhibition, Characterization and Purification of α-AMY from Sera of Iraqi Breast Cancer Patients

Lafta¹,

Al-Qaisi²

2023

The Egyptian Journal of Hospital Medicine

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Background: α-Amylase enzyme is digestive enzyme that breaks down complex carbohydrates like starch into simpler sugars; it is being studied as a potential target for anti-cancer therapies. Objective: The current study aimed to evaluate the general characterization, and inhibition of α-Amylase enzyme, which had been purified from the sera of patients diagnosed with Breast Carcinoma. Materials and methods: A total of 100 blood serum samples were collected from patients with Breast cancer from Baghdad National Hope Hospital in Baghdad. α-Amylase enzyme was purified from serum clinical isolate in three stages; precipitation with 65% saturated ammonium sulphate, ion exchange chromatography utilizing a DEAEcellulose column, and gel filtration chromatography with a sephadex G-200 column. Changing conditions in pH, temperature, inhibitor concentration, and kinetics all affect the activity of the α-Amylase enzyme. Results: α-Amylase inhibition assay showed that Vitis vinifera L. was the most potent inhibitor (71.8%). During the kinetic investigation of the enzyme, it was discovered that the inhibitory mechanism that the extracts use is noncompetitive. Vmax was 0.023, and 0.012 mmol min -l at 75 μg/ml of Vitis vinifera L. , Verbena Officinalis L .extracts, respectively. Conclusions: High specific activity for α-Amylase purified from sera of Iraqi breast cancer patients was obtained after three purification steps. Ethanolic plant extracts showed inhibitory effect on α-Amylase while Vitis vinifera L. was the most potent inhibitor.

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Twenty Traditional Algerian Plants Used in Diabetes Therapy as Strong Inhibitors of α-Amylase Activity

Cited by 21 publications

References 28 publications

Phytochemical Constituents and Antioxidant Activity of Oudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage

Phytochemical Constituents and Antioxidant Activity of Oudneya Africana L. Leaves Extracts: Evaluation Effects on Fatty Acids and Proteins Oxidation of Beef Burger during Refrigerated Storage

In silico and in vitro assessment on antidiabetic efficacy of secondary metabolites from Syzygium cumini (L.) Skeels

Inhibition, Characterization and Purification of α-AMY from Sera of Iraqi Breast Cancer Patients

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