Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation

Heyden, Marcel A.G. van der; Defize, L. H. K.

doi:10.1016/s0008-6363(02)00771-x

Cited by 173 publications

(144 citation statements)

References 85 publications

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“…P19 cells are a useful model system for investigating the roles of various factors in cardiac induction and differentiation (12). P19 cells also represent an excellent model system for studying the regulation of myocardial electrophysiological differentiation at the molecular and functional levels (21).…”

Section: Discussionmentioning

confidence: 99%

“…P19 cells, which are isolated from an experimental embryo-derived teratocarcinoma in mice, are multipotent and can differentiate into the cell types of all 3 germ layers. Due to their ability to form cardiomyocytes, P19 cells have been used to understand the role of cardiac-specific transcription factors and upstream signaling pathways in cardiac cell differentiation (12). We constructed vectors that overexpressed GATA-4 and silenced RNA interference.…”

Section: Introductionmentioning

confidence: 99%

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GATA-4 promotes the differentiation of P19 cells into cardiac myocytes

Chen²,

Liu³

et al. 2010

Int J Mol Med

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Abstract. The aim of this study was to investigate the effects of GATA-4 on the differentiation of P19 cells into cardiomyocytes and to examine the relationship between GATA-4 and cardiomyocytes. We constructed vectors to overexpress and silence GATA-4. These vectors, as well as empty ones were transfected into P19 cells. Subsequently, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis were performed. The morphology of P19 cells during differentiation was observed using an inverted microscope. Total RNA was extracted from P19 cells. We used real-time PCR to evaluate the expression levels of 6 genes: GATA-4, GATA-6, transthyretin (TTR), ·-fetoprotein (AFP), Nkx2.5, and ·-myosin heavy chain (·-MHC). The gene expression pattern of these 6 genes is graphically shown for each group. The GATA-4 mRNA level in cells overexpressing GATA-4 was notably higher than that in the controls, whereas the levels in the controls were notably higher than those in the GATA-4-silenced P19 cells. The cell lines overexpressing GATA-4 expressed higher levels of Nkx2.5 and ·-MHC than the controls. However, the controls expressed higher levels of AFP, GATA-6 and TTR than the cells overexpressing GATA-4. The RNAi group expressed lower levels of TTR, Nkx2.5, and ·-MHC than the controls, but there were no differences in the RNAi group and the controls with regard to the expression levels of AFP and GATA-6. The gene expression pattern in the cells overexpressing GATA-4 was biased toward the Nkx2.5 and ·-MHC. On the other hand, the gene expression pattern in GATA-4-silenced cells and the controls was biased toward the TTR and AFP. The overexpression of GATA-4 enhances the differentiation of P19 cells into cardiac myocytes, whereas its down-regulation suppresses this trend.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GATA-4 promotes the differentiation of P19 cells into cardiac myocytes

Chen²,

Liu³

et al. 2010

Int J Mol Med

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“…In our study, two well-known embryonic stem cell lines were used for EB formation and differentiation. We particularly used the murine P19 embryonal carcinoma cells, which are pluripotent and more extensively characterized stem cells that can be induced to differentiate in vitro into multiple cell types, especially neural and cardiac cells (McBurney 1993;Skerjanc 1999;Van der Heyden and Defize 2003). The differentiation of P19 cells into neural cells or cardiomyocytes is initiated by allowing the cells to aggregate in suspension culture in the presence of different inducers (Skerjanc 1999).…”

Section: Discussionmentioning

confidence: 99%

Formation of embryoid bodies from mouse embryonic stem cells cultured on silicon-coated surfaces

et al. 2009

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Embryoid bodies (EBs) are primitive embryonic structures derived from differentiating embryonic stem cells (ESCs). Many techniques have been used to obtain EBs. Improving the technique of EB formation can help in achieving better results in ESCs differentiation into neurons, myocardiocytes, haemopoeitic cells, and others. We evaluated the use of Sigmacote TM as a hydrophobic substrate to improve EB formation. CCE and P19 cell lines were used to obtain EBs and retinoic acid was used to induce neural differentiation. The results revealed that Sigmacote TM , as a hydrophobic substrate, can improve EB formation from ESCs. Our results demonstrate that the siliconcoating of glass petri dishes by Sigmacote TM is an easy and reproducible technique to enhance EB formation from murine ESCs and EC cells.

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“…Re-establishing Zrf1 expression in Zrf1 knockdown cell lines indicates that it is directly involved in the formation of these tissues. In particular, Zrf1 is essential for proper development of cardiac tissue as experiments with mESCs and P19 cells 46,47 indicate. Mechanistically, Zrf1 binds to the transcriptional start sites (TSSs) of heart tissue-specific genes where it drives their temporal expression during differentiation.…”

Section: 26mentioning

confidence: 99%

“…To further emphasize our findings we next investigated a potential Zrf1 function in P19 cells. P19 cells are a pluripotent cell line derived from a teratocarcinoma induced in C3H/HeHa mice, 46,47,51 which are able to differentiate into a variety of cell types representative of all 3 germ layers when induced by chemical agents. 47 In particular, P19 cells provide an excellent cell differentiation model system that mimics the events of early cardio-embryogenesis.…”

Section: Zrf1 Is Essential For Cardiomyocyte Differentiation In P19 Cmentioning

confidence: 99%

Zrf1 controls mesoderm lineage genes and cardiomyocyte differentiation

Kaymak

Richly

2016

Cell Cycle

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In the present study we addressed the function of the transcriptional activator Zrf1 in the generation of the 3 germ layers during in vitro development. Currently, Zrf1 is rather regarded as a factor that drives the expression of neuronal genes. Here, we have employed mouse embryonic stem cells and P19 cells to understand the role of Zrf1 in the generation of mesoderm-derived tissues like adipocytes, cartilage and heart. Our data shows that Zrf1 is essential for the transcriptional activation of genes that give rise to mesoderm and in particular heart development. In both, the mESC and P19 systems, we provide evidence that Zrf1 contributes to the generation of functional cardiomyocytes. We further demonstrate that Zrf1 binds to the transcription start sites (TSSs) of heart tissue-specific genes from the first and second heart field where it drives their temporal expression during differentiation. Taken together, we have identified Zrf1 as a novel regulator of the mesodermal lineage that might facilitate spatiotemporal expression of genes.

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Twenty one years of P19 cells: what an embryonal carcinoma cell line taught us about cardiomyocyte differentiation

Cited by 173 publications

References 85 publications

GATA-4 promotes the differentiation of P19 cells into cardiac myocytes

GATA-4 promotes the differentiation of P19 cells into cardiac myocytes

Formation of embryoid bodies from mouse embryonic stem cells cultured on silicon-coated surfaces

Zrf1 controls mesoderm lineage genes and cardiomyocyte differentiation

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