Twenty-four novel polymorphic microsatellite markers from oriental river prawn (Macrobrachium nipponense)

Y., K.; Feng, Jianbin; Li, J. L.; Ding, Huai-Yu

doi:10.1007/s12686-010-9236-z

Cited by 7 publications

(5 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The average number of polymorphic alleles per locus was 3.5, which was similar to the result of Cao et al (2012) for F. penicillatus, but it was lower than that reported for other shrimps (Xu et al, 2001;Meng et al, 2009;Ma et al, 2010). The PIC value is an important indicator that measures the extent of polymorphism for marker genes or marker sequences (Lü, 1994).…”

Section: Resultssupporting

confidence: 80%

“…Microsatellite markers are widely used in population differentiation, population genetics, linkage analyses, and evolutionary studies (Li, 2006). Microsatellite markers in other shrimps have been reported, including Litopenaeus vannamei (Garcla and Alcivar-Warren, 1996), Penaeus monodon (Xu et al, 2001), F. chinensis (Meng et al, 2009), and Macrobrachium nipponense (Ma et al, 2010). To the best of our knowledge, there are a few reports referring F. penicillatus, while major studies focusing on the cultural ecology, basic biology, as well as breeding (Zhang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus

Ning

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Fenneropenaeus penicillatus is one of the major economic shrimp species in China. In this study, 14 novel microsatellite loci were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphisms were tested in 30 individuals from a single-wild population. The results showed that the number of alleles at each locus ranged from two to four, and the polymorphism information content varied from 0.314 to 0.692. The observed and expected heterozygosities ranged from 0.3343 to 0.6542 and from 0.3458 to 0.6657, respectively. Three loci deviated significantly from Hardy-Weinberg equilibrium after a Bonferroni correction was applied, while no deviations were detected in the other 11 loci. The new microsatellite loci identified in this study could be useful in future F. penicillatus population genetic, conservation research, population structure assessment, and linkage map construction studies.

show abstract

Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus

Ning

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…One possible reason for this phenomenon is that there are more inbred individuals in the population, and the population may decompose into a series of family groups with closer genetic relationships or inbreeding. Heterozygosity due to inbreeding can lead to segregation of genes in the offspring and a tendency for the genetic composition of the offspring population to be pure, with most of the lack of heterozygote (excess of homozygote) due to null alleles, ultimately leading to loci that deviate from the Hardy-Weinberg equilibrium [29]. This result is consistent with the reality of the offspring population in this study.…”

Section: Contribution Rate Of Different Parents To Offspring Populationsupporting

confidence: 88%

Establishment of Parentage Identification Method for Sea Urchin Strongylocentrotus intermedius Based on SSR-seq Technology

Jiang,

Liu,

Guo

et al. 2024

Genes

View full text Add to dashboard Cite

To establish a parentage identification method for Strongylocentrotus intermedius, 15 microsatellite loci and simple sequence repeat sequencing (SSR-seq) technology were used to perform SSR sequencing and typing of the validation population with known pedigree information and the simulation population. Cervus v3.0 was used for gene frequency statistics, simulated analysis, and parentage identification analysis. The results showed that, in validation population, using 15 microsatellite loci, the highest success rate of parent pairs identification was 86%, the highest success rate of female parent identification was 93%, and the highest success rate of male parent identification was 90%. The simulated population was analyzed using 12–15 loci, and the identification rate was up to 90%. In cases where accurate parentage was not achieved, individuals could exhibit genetic similarities with 1–3 male or female parents. Individuals identified as lacking a genetic relationship can be selected as parents to prevent inbreeding. This study shows that parent pairs or single parents of most offspring can be identified successfully using these 15 selected loci. The results lay a foundation for the establishment of a parentage identification method for S. intermedius.

show abstract

“…Nine previously isolated microsatellite markers in M. nipponense (Ma et al, 2010(Ma et al, , 2011 were selected to genotype all samples. Polymerase chain reactions (PCR) were performed in 10-µL volumes including: 0.25 U Taq DNA polymerase (Tiangen, China), 1X Taq DNA polymerase PCR buffer (10 mM Tris-HCl, 1.5 mM MgCl 2 , 50 mM KCl, pH 8.3), 1 µM of each primer, 200 µM dNTPs and 50 ng template DNA.…”

Section: Dna Extraction and Microsatellite Analysismentioning

confidence: 99%

Genetic variation based on microsatellite analysis of the oriental river prawn, Macrobrachium nipponense from Qiandao Lake in China

Y¹,

Feng²,

Li³

2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The oriental river prawn (Macrobrachium nipponense) is an important freshwater prawn species in China. We collected 236 oriental river prawns from four wild stocks from Qiandao Lake, Zhejiang, China, and used nine polymorphic microsatellite markers to investigate their genetic diversity and structure, to facilitate the development of a selective breeding program. We found 185 alleles at nine loci in this sample. The observed heterozygosity (H O ) and expected heterozygosity (H E ) ranged from 0.43 to 0.89 and 0.56 to 0.95, respectively. The four stocks of M. nipponense displayed high genetic diversity (14.33-15.89 alleles/locus, H O = 0.66-0.77 and H E = 0.78-0.88). Genetic diversity of the stock from Weiping town was lower than the stocks from the other locations. Mutation-drift equilibrium analysis showed no significant bottleneck effect. F-statistics among stocks ranged from 0.03 to 0.07, indicating a moderate level of differentiation. Based on genetic structure analysis, the 236 individuals from the four wild stocks could be divided into two potential populations. Overall, the 09CA, 09AY and 09JJ wild stocks had higher allelic and genetic diversity than the upstream 09WP stock. These three wild stocks could be used as founders for selective breeding.

show abstract

Twenty-four novel polymorphic microsatellite markers from oriental river prawn (Macrobrachium nipponense)

Cited by 7 publications

References 3 publications

Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus

Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus

Establishment of Parentage Identification Method for Sea Urchin Strongylocentrotus intermedius Based on SSR-seq Technology

Genetic variation based on microsatellite analysis of the oriental river prawn, Macrobrachium nipponense from Qiandao Lake in China

Contact Info

Product

Resources

About