Twenty-Eight Element Concentrations in Mane Hair Samples of Adult Riding Horses Determined by Particle-Induced X-Ray Emission

Asano, Kazushi; Suzuki, Kazuyuki; Chiba, Momoko; Sera, K.; Asano, Ryuji; Sakai, Takeo

doi:10.1385/bter:107:2:135

Cited by 31 publications

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“…The horses were assigned to either the control (n=22, no abnormalities) or AF groups (n=5, subclinically established atrial fibrillation) based on ECG findings. In a previous study, we demonstrated that the values obtained are not dependent on the horse's age, breed, and sex, and that they may be used as reference values in the assessment of diseases and nutritional status in horses [2]. However, the influence of hair color on elemental status has already been demonstrated in the horse [1,5].…”

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Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Asano

Suzuki

Chiba

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. To investigate the relationships between trace elements concentrations in hair and atrial fibrillation (AF) in horses, concentrations of nineteen trace elements were detected in hair using the particle induced X-ray emission (PIXE) method. The horses were assigned to either control (n=22, no abnormalities) or AF groups (n=5) based on electrocardiograph findings. The mean concentrations of Ca and Zn in the hair of the AF group were significantly higher than those in the control group. The Zn/Cu ratio of the hair in the AF group (29.8 ± 5.5) was significantly higher than that in the control group (23.4 ± 2.2, P<0.05). The results of the present study suggest that there is a relationship between elevated concentrations of Ca and Zn in hair and AF. KEY WORDS: atrial fibrillation, equine, trace element.J. Vet. Med. Sci. 68 (7): [769][770][771] 2006 Atrial fibrillation (AF) is the most common clinically relevant cardiac arrhythmia in horses [10]. Using electrocardiography, AF is identified by the lack of definitive P waves, presence of deflections of variable frequency and amplitude in the baseline (f waves) attributable to chaotic electrical activity in the atria, and an irregular ventricular rate. Older horses with AF often have signs of congestive heart failure, prominent murmurs, and a rapid resting heart rate [6,9].Many reports have discussed the relevance of Zn in arrhythmias in human [11], guinea pigs [4,8] and rats [14]. We previously described that horses with first and second degrees atrioventricular (AV) blocks have a large amount of Zn in hair compared to those without arrhythmia [3]. Those results suggested that evaluation of the concentration of Zn in hair might be used to predict the susceptibility of horses to heart disease much earlier than the appearance of symptoms and development of heart disease. However, correlations between arrhythmia and element status in hair are not yet clear, except in the case of AV block.Therefore, investigation of the relationship between AF and trace element status is important for equine health care. The aim of this study was to investigate the relationships between mean concentrations of those elements and AF in the mane hair of horses.Experiments were performed on 27 castrated and bay horses, 13.2 ± 4.2 year-old. The horses used in this study were kept under the same management conditions for at least three months in the same riding club (Yokohama, Kanagawa) and were deemed healthy on the basis of physical examinations. Electrocardiograms (ECG) of the resting horses were taken by a portable cardiac monitor (PAM Cardiac Monitor VM-8000, West Lagayette, IN, U.S.A.) and recorded for one minute in a stable. The horses were assigned to either the control (n=22, no abnormalities) or AF groups (n=5, subclinically established atrial fibrillation) based on ECG findings. In a previous study, we demonstrated that the values obtained are not dependent on the horse's age, breed, and sex, and that they may be used as reference values in the assessment of diseases and n...

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“…A total of 10 hairs were collected as hair specimens from the nape area nearest to the skin of each horse. The hair specimens were further cut to even lengths of about 6 cm and washed with acetone a few times [1][2][3]. Only 10 hairs were attached per holder and directly irradiated with proton beams.…”

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Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Asano

Suzuki

Chiba

et al. 2006

The Journal of Veterinary Medical Science

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“…Os valores encontrados nesse estudo foram maiores do que os relatados por Asano et al (2005), os quais obtiveram médias de 1362 ± 457 µg/g para cavalos meio-sangue.…”

Section: Cálcio (Ppm)unclassified

“…No pêlo dos animais, houve efeito quadrático (P<0,001) da concentração de Na, em relação ao período, independente dos tratamentos, e no presente estudo as concentrações médias ficaram acima das citadas por Asano et al (2005) para a categoria animal (246 ± 123 ppm).…”

Section: Sódiounclassified

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Minerais orgânicos na prevenção hiperparatireoidismo nutricional secundário em equinos

Wajnsztejn¹

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A Deus, em primeiro lugar.Sei que ninguém chega a algum lugar ou realiza algo sozinho. Portanto agradeço:Aos meus pais, Rubens Wajnsztejn e Norka Bonetti, irmãos, Eric Wajnsztejn e Victor Wajnsztejn, avós, Jankiel Wajnsztejn (in memorian) e Clara G. Wajnsztejn, Wagner Bonetti e Maud P. Bonetti (in memorian), tios, tias, primos e demais parentes, pelo amor incondicional.Ao meu orientador Prof. Dr. Alexandre Augusto de Oliveira Gobesso agradeço a oportunidade de realizar este trabalho, pela paciência, convívio e por todos os ensinamentos. À Fundação de Amparo à Pesquisa do Estado de São Paulo -FAPESP, pelofinanciamento do projeto de pesquisa e incentivo à experimentação animal.À Tortuga Cia Zootécnica Agrária, pelo fornecimento do material e financiamento do projeto. À Ourofino Saúde Animal e também a SL Alimentos, pela assistência.Aos 24 animais utilizados nesse estudo, e aos outros muitos que me proporcionaram conhecimento. Aos estagiários Carolina (Guapa), Jaqueline, Camilla e Eduardo, e a todos os graduandos que estiveram em Pirassununga ajudando em todas as fases.A todos os meus amigos de pós-graduação do VNP e da FZEA pelo apoio e horas boas que passamos juntos.Ao prof. Margutti e a todos os amigos dos treinos, pelos ensinamentos e momentos especiais nas práticas esportivas.Por estar sempre ao meu lado, compartilhando os momentos felizes e apoiando em outros menos felizes, agradeço à Paula, minha namorada.A todos aqueles que, direta ou indiretamente, estiveram presentes em mais essa etapa de minha vida, agradeço de coração. Não é raro criar equinos em áreas onde baixo teor de fósforo, indisponibilidade de cálcio e teor de oxalato nas plantas podem levar a desequilíbrios resultando em hiperparatireoidismo nutricional secundário. Normalmente, os minerais atuam no organismo na forma orgânica e não inorgânica, porém, ainda há controvérsia na literatura quanto ao uso de minerais orgânicos. Objetivando-se avaliar o efeito da adição de ácido oxálico na dieta, causando desequilíbrio entre cálcio e fósforo em potros, e se dietas com minerais orgânicos, quando comparadas a dietas com minerais inorgânicos, seriam capazes de evitar esse desequilíbrio e evitar o desenvolvimento da patologia, analisaram-se: pesos dos animais, parâmetros sorológicos, concentrações minerais nos pêlos, densidade mineral óssea e biópsias ósseas.Utilizaram-se 24 potros, sem raça definida, com idade entre 18 e 24 meses. Cada tratamento foi composto por 6 potros (3 machos e 3 fêmeas), totalizando 4 tratamentos, em delineamento inteiramente casualizado, com medidas repetidas no tempo, em arranjo fatorial 2x2: suplementação com minerais orgânicos ou minerais inorgânicos, e presença ou ausência de oxalato na dieta. A colheita de amostras foi realizada em 150 dias, sendo a pesagem a cada 15 dias, os parâmetros sorológicos, mineralogramas capilares e densitometrias ósseas a cada 30 dias, e biópsias ósseas a cada 75 dias. Os resultados demonstraram aumento linear dos pesos em relação aos períodos, independente dos tratamentos e não foram observadas di...

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Relationship between hair elements and severity of atrioventricular block in horses

Suzuki

Yamaya

Asano

et al. 2007

Biol Trace Elem Res

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The aim of this study was to investigate the relationships between the mean concentrations of trace elements and the severity of the second-degree atrioventricular (AV) block in the mane hair of horses. Electrocardiographs of horses were continually recorded for 6 h using a holter cardiac monitor to determine dropped ventricular beats (DVBs) which can be used as an indicator of the severity of the AV block. Mane hair Ca, Cu, Mg, and Zn concentrations were measured by the particle-induced X-ray emission method. The Zn/Cu ratio and Ca concentration in mane hair were significantly and positively correlated with the hourly DVBs in horse with a second-degree AV block (p<0.01, r2=0.485; p<0.05, r2=0.351, respectively). Proposed diagnostic cutoff points for hair Ca concentration and Zn/Cu ratio based on receiver operating characteristics curves analysis in detecting second-degree AV block were set at 1536 microg/g and 26.0, respectively. Those results with horse hair suggest that the evaluation of the Ca, Cu, Mg, and Zn status in mane hair by this method is strongly related to the severity of second-degree AV block and might predict the susceptibility of an individual much before the development of the symptom.

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Twenty-Eight Element Concentrations in Mane Hair Samples of Adult Riding Horses Determined by Particle-Induced X-Ray Emission

Cited by 31 publications

References 20 publications

Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Relationship between Trace Elements Status in Mane Hair and Atrial Fibrillation in Horse

Minerais orgânicos na prevenção hiperparatireoidismo nutricional secundário em equinos

Relationship between hair elements and severity of atrioventricular block in horses

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