Twelve New Seco-Pregnane Glycosides from Cynanchum taihangense

Abstract

For our interest in the potential biologically active and structurally unique steroidal glycosides, continued phytochemical investigation of Cynanchum taihangense was carried out; twelve new seco-pregnane glycosides, cynataihosides I-L (1–4), M-T (7–14), and two known glycosides, glaucoside A (5) and atratcynoside F (6), were isolated from the 95% ethanol extract of Cynanchum taihangense. Two new aglycones were found among compounds 10, 11, 13, and 14. The structures of the glycosides were elucidated based on 1D and 2D NMR spectroscopic data, HR-ESI-MS analysis, and chemical evidence. The cytotoxicity of compounds against three human tumor cell lines (HL-60, THP-1, and PC-3) were evaluated by MTT assay. Compound 11 displayed significant cytotoxicity against THP-1 and PC-3 cell line with IC50 values of 5.08 and 22.75 μm, respectively. Compounds 3 and 14 exhibited moderate and selective cytotoxicity on HL-60 and THP-1 with IC50 values of 17.78 and 16.02 μm, respectively.

New Pregnane Glycosides from Mandevilla dardanoi and Their Anti-Inflammatory Activity

“…Composed of a steroidal scaffold, the pregnane (C 21 ) and seco-pregnane-type glycosides mainly occur in Apocynaceae, Malpighiaceae, Ranunculaceae, and Zygophyllaceae families [11]. This class of compounds has demonstrated remarkable biological activities, including anticancer, antinociceptive, anti-inflammatory, antiviral, and antibacterial properties [12][13][14][15][16]. Considering the medicinal potential of Mandevilla species, the current study aimed to isolate novel bioactive pregnane derivatives from the roots of M. dardanoi.…”