Twelve actin-encoding cDNAs from the American lobster, Homarus americanus: Cloning and tissue expression of eight skeletal muscle, one heart, and three cytoplasmic isoforms

Kim, Bo Kwang; Kim, Kyoung Sun; Oh, Chul‐Woong; Mykles, Donald L.; Lee, Sung Gu; Kim, Soo Hyun; Kim, Hyun Woo

doi:10.1016/j.cbpb.2009.02.013

Cited by 25 publications

(22 citation statements)

References 32 publications

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“…Amongst ESTs assigned molecular functions, many were assigned binding (45.9%) or catalytic functions (32.3%), predominantly actin and zinc ion proteins ( Table S2 ). Recent studies of crustaceans have highlighted the importance of actin in constructing muscle tissues and that it shows variable expression in different muscle types [39]–[42]. The cellular component assignments showed many EST coding sequences to likely possess cell (22.8%) and cell part (22.5%) functions, whilst those assigned biological functions were mostly predicted to be involved in cellular (17.6%) or metabolic processes (16.5%) including proteolysis, carbohydrate metabolism or oxidation-reductive functions.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomics of a Giant Freshwater Prawn (Macrobrachium rosenbergii): De Novo Assembly, Annotation and Marker Discovery

et al. 2011

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BackgroundGiant freshwater prawn (Macrobrachium rosenbergii or GFP), is the most economically important freshwater crustacean species. However, as little is known about its genome, 454 pyrosequencing of cDNA was undertaken to characterise its transcriptome and identify genes important for growth.Methodology and Principal FindingsA collection of 787,731 sequence reads (244.37 Mb) obtained from 454 pyrosequencing analysis of cDNA prepared from muscle, ovary and testis tissues taken from 18 adult prawns was assembled into 123,534 expressed sequence tags (ESTs). Of these, 46% of the 8,411 contigs and 19% of 115,123 singletons possessed high similarity to sequences in the GenBank non-redundant database, with most significant (E value < 1e–5) contig (80%) and singleton (84%) matches occurring with crustacean and insect sequences. KEGG analysis of the contig open reading frames identified putative members of several biological pathways potentially important for growth. The top InterProScan domains detected included RNA recognition motifs, serine/threonine-protein kinase-like domains, actin-like families, and zinc finger domains. Transcripts derived from genes such as actin, myosin heavy and light chain, tropomyosin and troponin with fundamental roles in muscle development and construction were abundant. Amongst the contigs, 834 single nucleotide polymorphisms, 1198 indels and 658 simple sequence repeats motifs were also identified.ConclusionsThe M. rosenbergii transcriptome data reported here should provide an invaluable resource for improving our understanding of this species' genome structure and biology. The data will also instruct future functional studies to manipulate or select for genes influencing growth that should find practical applications in aquaculture breeding programs.

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Section: Resultsmentioning

confidence: 99%

Transcriptomics of a Giant Freshwater Prawn (Macrobrachium rosenbergii): De Novo Assembly, Annotation and Marker Discovery

et al. 2011

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“…Actin isoforms may consist of skeletal muscle actins or cytoskeletal actins. Kim et al (2009) reported 12 actin isoforms in the American lobster Homarus americanus, of which eight were skeletal, one was cardiac and three were cytoskeletal/cytoplasmic isoforms. Although the isoform identity of the actin sequence for P. varians is unresolved, it was found to be highly expressed in the muscle-rich tissue of the shrimp's abdominal section and was also expressed in the head section.…”

Section: Discussionmentioning

confidence: 99%

Characterising multi-level effects of an acute pressure exposure on a shallow-water invertebrate: insights into the kinetics and hierarchy of the stress response

Morris

Thatje

Ravaux

et al. 2015

Journal of Experimental Biology

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Hydrostatic pressure is an important, ubiquitous, environmental variable of particular relevance in the marine environment. However, it is widely overlooked despite recent evidence that some marine ectotherms may be demonstrating climate-driven bathymetric range shifts. Wide-ranging effects of increased hydrostatic pressure have been observed from the molecular through to the behavioural level. Still, no study has simultaneously examined these multiple levels of organisation in a single experiment in order to understand the kinetics, hierarchy and interconnected nature of such responses during an acute exposure, and over a subsequent recovery period. Here, we quantify the transcription of a set of previously characterised genes during and after acute pressure exposure in adults of the shrimp Palaemonetes varians. Further, we perform respiratory rate and behavioural analysis over the same period. Increases in expression of genes associated with stress and metabolism were observed during and after high-pressure exposure. Respiratory rate increased during exposure and into the recovery period. Finally, differential behaviour was observed under elevated hydrostatic pressure in comparison to ambient pressure. Characterising generalised responses to acute elevated pressure is a vital precursor to longer-term, acclimation-based pressure studies. Results provide a novel insight into what we term the overall stress response (OSR) to elevated pressure; a concept that we suggest to be applicable to other environmental stressors. We highlight the importance of considering more than a single component of the stress response in physiological studies, particularly in an era where environmental multi-stressor studies are proliferating.

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“…Twelve complete actin open reading frames belonging to three types of actin -skeletal, cardiac and cytoskeletal -were identified in H. americanus (Kim et al, 2009), but no distinction of P. leptodactylus actin types was found by BLASTP comparisons of the seven partial actin transcripts with those of H. americanus. Projectin is a long protein that binds filamentous actin and provides elasticity to muscles, and at present, it is assigned a function only in the muscular context (Ayme-Southgate et al, 2008).…”

Section: Research Articlementioning

confidence: 98%

Different transcription regulation routes are exerted by L- and D-amino acid enantiomers of peptide hormones

Tom

Manfrin

Mosco

et al. 2014

Journal of Experimental Biology

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Conversion of one or more amino acids in eukaryotic peptides to the D-enantiomer configuration is catalyzed by specific L/D-peptide isomerases and it is a poorly investigated post-translational modification. No common modified amino acid or specific modified position has been recognized, and mechanisms underlying changes in the peptide function provided by this conversion are not widely studied. The 72 amino acid crustacean hyperglycemic hormone (CHH) in Astacidea crustaceans exhibits a co-existence of two peptide enantiomers with either D-or L-phenylalanine as their third residue. It is a pleiotropic hormone regulating several physiological processes in different target tissues and along different time scales. CHH enantiomers differently affect time courses and intensities of examined processes. The short-term effects of the two isomers on gene expression were examined in the hepatopancreas, gills, hemocytes and muscles of the astacid Pontastacus leptodactylus. Gene expression in muscles and hemocytes was not affected by either of the isomers. Two modes of action for CHH were elucidated in the hepatopancreas and the gills: specific gene induction in both organs by D-CHH, and targeted attenuation caused by both enantiomers in the gills. Consequently, a two-receptor system is proposed for conveying the effect of the two CHH isomers.

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Twelve actin-encoding cDNAs from the American lobster, Homarus americanus: Cloning and tissue expression of eight skeletal muscle, one heart, and three cytoplasmic isoforms

Cited by 25 publications

References 32 publications

Transcriptomics of a Giant Freshwater Prawn (Macrobrachium rosenbergii): De Novo Assembly, Annotation and Marker Discovery

Transcriptomics of a Giant Freshwater Prawn (Macrobrachium rosenbergii): De Novo Assembly, Annotation and Marker Discovery

Characterising multi-level effects of an acute pressure exposure on a shallow-water invertebrate: insights into the kinetics and hierarchy of the stress response

Different transcription regulation routes are exerted by L- and D-amino acid enantiomers of peptide hormones

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