Tween-80 enhanced biodegradation of naphthalene by Klebsiella quasipneumoniae

Olukanni, Olumide D.; Albert, Anthony A.; Farinto, Micheal; Awotula, Ayodeji O.; Osuntoki, Akinniyi A.

doi:10.1007/s10482-023-01839-8

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…Bakteri dalam genus Klebsiella tersebar luas di alam dengan beberapa strain terbukti cocok untuk melakukan bioremediasi karena pertumbuhannya yang cepat, kapsul yang tebal pada permukaan dinding sel, dan ketahanan terhadap polutan (Zhang et al, 2019). Bakteri genus Klebsiella terbukti bisa menjadi pilihan yang terjangkau, efisien, dan ramah lingkungan dalam mereduksi bahkan menghilangkan kontaminan (Olukanni et al, 2023).…”

Section: Gambar 3 Pohon Filogeni Dari Isolat Bakteri Cf6 Dan Bakteri ...unclassified

Identifikasi Secara Fenotipik dan Genomik Isolat Bakteri Potensial Pendegradasi Herbisida Glifosat dari Rhizosfer Cabai Rawit

Maulida,

Lisdiana

2024

LenteraBio

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Bakteri merupakan salah satu agen bioremediasi untuk mengatasi pencemaran di lahan pertanian. Bakteri memiliki kemampuan mendegradasi senyawa kontaminan. Isolat bakteri CF6 merupakan isolat bakteri rhizosfer dari tanah pertanian cabai rawit yang berpotensi mampu mendegradasi herbisida glifosat. Namun, perlu diidentifikasi untuk diaplikasikan sebagai agen bioremediasi dan dipahami pengaruhnya terhadap ekosistem. Tujuan penelitian ini adalah mengidentifikasi secara fenotipik dan genomik isolat bakteri rhizosfer yang berpotensi sebagai pendegradasi herbisida glifosat sehingga dapat diaplikasikan lebih lanjut. Metode identifikasi secara fenotipik terdiri dari uji motilitas, pewarnaan gram, katalase, sitrat, urease, endospora, Voges-Proskauer, produksi indol, methyl red, pertumbuhan pada 5% NaCl, dan uji reduksi gula. Metode identifikasi secara genomik terbagi empat tahap, yaitu isolasi DNA, amplifikasi gen 16S rRNA, elektroforesis, dan sekuensing gen 16S rRNA. Analisis data dari hasil uji fenotipik secara numerik menggunakan NTSys 2.11a dan hasil uji genomik menggunakan MEGA11. Hasil dendrogram dalam penelitian ini menunjukkan isolat bakteri CF6 termasuk dalam satu klad dengan bakteri-bakteri anggota genus Klebsiella. Sedangkan hasil analisis BLASTn menunjukkan isolat uji CF6 memiliki kesamaan sekuen gen 16S rRNA dengan Klebsiella quasipneumoniae sebesar 98,62%. Dari hasil kedua metode identifikasi tersebut dapat disimpulkan bahwa isolat bakteri CF6 adalah bakteri K. quasipneumoniae. Aspek patogenitas dan laju degradasi bakteri tersebut perlu diuji sebelum diimplementasikan.

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Section: Gambar 3 Pohon Filogeni Dari Isolat Bakteri Cf6 Dan Bakteri ...unclassified

Identifikasi Secara Fenotipik dan Genomik Isolat Bakteri Potensial Pendegradasi Herbisida Glifosat dari Rhizosfer Cabai Rawit

Maulida,

Lisdiana

2024

LenteraBio

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Exploring the additive effect of Ampelopsis grossedentata flavonoids and Tween 80 on feeding Nubian goats

Zhu,

Lu,

Gao

et al. 2024

Front. Vet. Sci.

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IntroductionThe ban on antibiotics in animal husbandry underscores the crucial need for safe, natural feed additives. This study investigates the effects of Ampelopsis grossedentata flavonoids (AGF) and Tween 80 on the growth performance, blood indexes, and rumen microbiota of Nubian goats, evaluating their potential as alternative feed additives in livestock management.MethodsThirty-two goats were randomly divided into four groups. The control group (CON group) was provided with a basal diet, while the experimental groups received diets supplemented with various dietary additives for a duration of 100 days: either a basal diet supplemented with 25 mg/kg of monensin (MN group), a basal diet containing 2.0 g/kg of Ampelopsis grossedentata flavonoids (AGF group), or a basal diet containing 7.5 mL/kg of Tween 80 (TW group). Blood and rumen fluid samples were collected for analysis at the end of the feeding period. Growth performance was monitored through regular weighing and feed intake measurements. Blood indexes were analyzed using standard biochemical techniques, while the microbial composition of the rumen fluid was determined through high throughput 16S rRNA gene sequencing to assess microbial diversity and function. The effects of the dietary treatments on growth performance, blood indexes, and rumen microbial composition were then evaluated.ResultsThe AGF group exhibited significantly increased average daily gain, and decreased feed-to-gain ratio (p < 0.05). Blood indexes analysis revealed no differences between the CON and AGF groups, with both showing higher concentrations of triglyceride, low-density lipoprotein cholesterol, glutamic-pyruvic transaminase, alkaline phosphatase, and lactate dehydrogenase compared to the monensin group (p < 0.05). The TW group had significantly higher glucose, glutamic-oxaloacetic transaminase, and glutamic-pyruvic transaminase levels than the MN group (p < 0.05). Microbial diversity analysis revealed that the TW group had significantly greater alpha-diversity than other groups, while beta-diversity analysis showed closer similarity between the rumen microbiota of the AGF and CON groups. LEfSe analysis identified Proteobacteria, Deferribacteres, Ehryarchaeoia, and Elusimicrobia as biomarkers distinguishing the rumen microbiota among the groups. In conclusion, AGF supplementation increased the relative abundance of beneficial bacteria in the rumen of Nubian goats, and thus enhanced the growth performance. TW supplementation significantly increased rumen microbial diversity and abundance, suggesting benefits for rumen health despite poor palatability. These findings highlight the potential of AGF as a new green additive with important implications for the efficiency and development of animal husbandry.

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Tween-80 enhanced biodegradation of naphthalene by Klebsiella quasipneumoniae

Cited by 2 publications

References 40 publications

Identifikasi Secara Fenotipik dan Genomik Isolat Bakteri Potensial Pendegradasi Herbisida Glifosat dari Rhizosfer Cabai Rawit

Identifikasi Secara Fenotipik dan Genomik Isolat Bakteri Potensial Pendegradasi Herbisida Glifosat dari Rhizosfer Cabai Rawit

Exploring the additive effect of Ampelopsis grossedentata flavonoids and Tween 80 on feeding Nubian goats

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