Turnover of ribosomal proteins in regenerating rat liver after partial hepatectomy

Nikolov, Emil N.; Dineva, Bistra; Dabeva, Mariana D.; Николов, Тодор

doi:10.1016/0020-711x(87)90326-0

Cited by 14 publications

(14 citation statements)

References 16 publications

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“…The coordinated turnover of these complexes may represent an energy-conservation strategy by the cell to avoid the presence of orphan subunits. For example, the regulation of turnover among protein and RNA ribosomal subunits had been previously established (20). For a few complexes, such as the Cop9 signalosome complex (CSN), we observed a broad range of turnover rates.…”

Section: Discussionmentioning

confidence: 73%

“…Unlike differentiated cells, a dividing cell line is in continuous need of protein production to supply newly generated daughter cells. The regeneration of liver mass that occurs through the proliferation of hepatic cells was shown to reduce apparent protein half-life (20). Additionally, the range of half-lives in HeLa cells appears to be much broader than the corresponding measurements in vivo, perhaps because some of the mechanisms that regulate protein turnover in vivo (e.g., autophagy and tissue regeneration) are absent in culture.…”

Section: Discussionmentioning

confidence: 99%

“…Labeling experiments were performed on 9-wk-old male mice. At this age, mice are fully developed and tissue growth and differentiation are expected to play minimal roles in protein turnover (20). Following a 7-d habituation period using unlabeled Spirulina, 15 N-labeled Spirulina was introduced as the sole dietary protein source for 32 d (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Traditionally, protein turnover has been studied by measuring the incorporation of radioactive, tracer amino acids into proteins or bulk tissues (16)(17)(18)(19)(20). The advent of modern proteomics has enabled scientists to use mass spectrometry to detect the incorporation of stable isotopes into proteins (21,22).…”

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confidence: 99%

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Analysis of proteome dynamics in the mouse brain

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Guan²,

Burlingame³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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447

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Fig. 2. (A)Interaction web of top-down and bottom-up effects in the eelgrass study system. The top predator is the sea otter (E. lutris), the mesopredators are crabs (Cancer spp. and Pugettia producta), the epiphyte mesograzers are primarily an isopod (I. resecata) and a sea slug (P. taylori), and algal epiphyte competitors of eelgrass primarily consist of chain-forming diatoms, and the red alga Smithora naiadum. Solid arrows indicate direct effects, dashed arrows indicate indirect effects, and the plus and minus symbols indicate positive and/or negative effects on trophic guilds and eelgrass condition. C, competitive interaction; T, trophic interaction. (Original artwork by A. C. Hughes.) (B-E) Survey results testing for the effects of sea otter density on eelgrass bed community properties (Tables S2 and S3). Elkhorn Slough (sea otters present and high nutrients) eelgrass beds (n = 4) are coded in red, and the Tomales Bay reference site (no sea otters, low nutrients) beds (n = 4) are coded in blue. (B) Crab biomass and size structure of two species of Cancer crabs; (C) grazer biomass per shoot and large grazer density; (D) algal epiphyte loading; and (E) aboveground and belowground eelgrass biomass. DW, dry weight; FW, fresh weight.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Analysis of proteome dynamics in the mouse brain

Price

Guan²,

Burlingame³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

335

447

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“…It is likely that a similar situation holds for ribosomes. The turnover times of ribosomes in plants is not known, but it is on the order of 3 d in mammalian liver (Hirsch and Hiatt, 1966;Nikolov et al, 1987).…”

Section: Ribosome Abundance Does Not Change Substantially During Diurmentioning

confidence: 99%

Diurnal Changes of Polysome Loading Track Sucrose Content in the Rosette of Wild-Type Arabidopsis and the Starchless pgm Mutant

et al. 2013

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Growth is driven by newly fixed carbon in the light, but at night it depends on reserves, like starch, that are laid down in the light. Unless plants coordinate their growth with diurnal changes in the carbon supply, they will experience acute carbon starvation during the night. Protein synthesis represents a major component of cellular growth. Polysome loading was investigated during the diurnal cycle, an extended night, and low CO 2 in Arabidopsis (Arabidopsis thaliana) Columbia (Col-0) and in the starchless phosphoglucomutase (pgm) mutant. In Col-0, polysome loading was 60% to 70% in the light, 40% to 45% for much of the night, and less than 20% in an extended night, while in pgm, it fell to less than 25% early in the night. Quantification of ribosomal RNA species using quantitative reverse transcription-polymerase chain reaction revealed that polysome loading remained high for much of the night in the cytosol, was strongly light dependent in the plastid, and was always high in mitochondria. The rosette sucrose content correlated with overall and with cytosolic polysome loading. Ribosome abundance did not show significant diurnal changes. However, compared with Col-0, pgm had decreased and increased abundance of plastidic and mitochondrial ribosomes, respectively. Incorporation of label from 13 CO 2 into protein confirmed that protein synthesis continues at a diminished rate in the dark. Modeling revealed that a decrease in polysome loading at night is required to balance protein synthesis with the availability of carbon from starch breakdown. Costs are also reduced by using amino acids that accumulated in the previous light period. These results uncover a tight coordination of protein synthesis with the momentary supply of carbon.

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Dynamic protein composition of Arabidopsis thaliana cytosolic ribosomes in response to sucrose feeding as revealed by label free MS^E proteomics

et al. 2012

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Cytosolic ribosomes are among the largest multisubunit cellular complexes. Arabidopsis thaliana ribosomes consist of 79 different ribosomal proteins (r-proteins) that each are encoded by two to six (paralogous) genes. It is unknown whether the paralogs are incorporated into the ribosome and whether the relative incorporation of r-protein paralogs varies in response to environmental cues. Immunopurified ribosomes were isolated from A. thaliana rosette leaves fed with sucrose. Trypsin digested samples were analyzed by qTOF-LC-MS using both MS(E) and classical MS/MS. Peptide features obtained by using these two methods were identified using MASCOT and Proteinlynx Global Server searching the theoretical sequences of A. thaliana proteins. The A. thaliana genome encodes 237 r-proteins and 69% of these were identified with proteotypic peptides for most of the identified proteins. These r-proteins were identified with average protein sequence coverage of 32% observed by MS(E) . Interestingly, the analysis shows that the abundance of r-protein paralogs in the ribosome changes in response to sucrose feeding. This is particularly evident for paralogous RPS3aA, RPS5A, RPL8B, and RACK1 proteins. These results show that protein synthesis in the A. thaliana cytosol involves a heterogeneous ribosomal population. The implications of these findings in the regulation of translation are discussed.

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Turnover of ribosomal proteins in regenerating rat liver after partial hepatectomy

Cited by 14 publications

References 16 publications

Analysis of proteome dynamics in the mouse brain

Analysis of proteome dynamics in the mouse brain

Diurnal Changes of Polysome Loading Track Sucrose Content in the Rosette of Wild-Type Arabidopsis and the Starchless pgm Mutant

Dynamic protein composition of Arabidopsis thaliana cytosolic ribosomes in response to sucrose feeding as revealed by label free MS^E proteomics

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Turnover of ribosomal proteins in regenerating rat liver after partial hepatectomy

Cited by 14 publications

References 16 publications

Analysis of proteome dynamics in the mouse brain

Analysis of proteome dynamics in the mouse brain

Diurnal Changes of Polysome Loading Track Sucrose Content in the Rosette of Wild-Type Arabidopsis and the Starchless pgm Mutant

Dynamic protein composition of Arabidopsis thaliana cytosolic ribosomes in response to sucrose feeding as revealed by label free MSE proteomics

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Dynamic protein composition of Arabidopsis thaliana cytosolic ribosomes in response to sucrose feeding as revealed by label free MS^E proteomics