Turnover de carbono nos tecidos de frangos de corte em diferentes fases de crescimento

Abstract

RESUMO Este trabalho objetivou determinar o turnover do carbono no 2,5; 2,8; 2,9; 4,9; 5,1; 5,5 e 6,0 dias para músculo peitoral; 2,7; 2,9; 3,1; 4,6; 5,3; 4,1 e 8,0 dias para quilha; 4,4; 3,6; 5,4; 5,8; 4,3; 5,2 e 6,3 dias para tíbia e 1,3; 1,9; 1,4; 1,5; 1,9; 1,7 (0, 7, 14, 21, 28, 35, 42 days old). Either Boltzmann sigmoid regression or the exponential time function were used in order to measure the carbon isotopic turnover in a specifi c period of time. 2.8; 2.9; 4.9; 5.1; 5.5 e 6.0 day on muscle tissue; 2.7; 2.9; 3.1; 4.6; 5.3; 4.1 e 8.0 day on keel; 4.4; 3.6; 5.4; 5.8; 4.3; 5.2 e 6.3 day on tibia and 1.3; 1.9; 1.4; 1.5; 1.9; 1.7

Estimation of the metabolic rate by assessing carbon-13 turnover in broiler tissues using the stable isotope technique

“…It is worth noting that half-life values in birds increase with age, as the growth rate (prevalence of synthesis rate over degradation rate) is higher in young animals (Gonzales and Sartori, 2002). A similar age-related increase in half-life values was observed by Ducatti et al (2014) assessing the turnover of 13 C in pectoral muscle, keel, tibia, and liver.…”

“…However, to develop these lines of research, further studies would be required to improve the knowledge of isotopic assimilation of organic matter in different bird' tissues and growth phases. Ducatti et al (2014) assessed 13 C turnover in pectoral muscle, keel, tibia, and liver to obtain physiological information that would be essential for traceability studies. However, the time of assessment and tissue harvest was not sufficient for these tissues to achieve a balanced level of carbon isotope exchange.…”

Dietary supplementation with glutamine or glutamic acid for weanling piglets

“…DFI, daily feed intake; DWG, daily weight gain; and F:G, feed:gain ratio. results average 13 C half-life value of 3.82 days were found by Ducatti et al (2014) for the Pectoralis major muscle of broilers at different periods of growth. Bahar et al (2009), studying two bovine muscles (Longissimus dorsi and Psoas major) reported 13 C half-life values of 133 and 157 days, respectively.…”

“…The data expressed the δ 13 C notation in relation to Pee Dee Belemnite (PDB), an international standard, with analysis deviation at the order of 0.2‰; δ 13 C were calculated by the equation: δ 13 C (sample, standard) = [(R sample / R standard ) -1] × 10 3 , in which δ 13 C is the enrichment of the isotopic ratio 13 C/ 12 C of the sample to the standard; and R represents the ratio of the heavier ( 13 C) to the lighter ( 12 C) stable isotopes (dimensionless). In order to evaluate the 13 C-substitution speed of the samples, the following exponential function over time was employed (Ducatti et al, 2014): δ 13 C (t) = δ 13 C (f) + [δ 13 C (i) -δ 13 C (f) ] e -kt , in which δ 13 C (t) is the isotopic enrichment of the tissue at any time (t); δ 13 C (f) is the isotopic enrichment of the tissue at the equilibrium or final condition; δ 13 C (i) is the isotopic enrichment of the tissue at the beginning; k is the turnover constant, in units of time -1 ; and t is the time (days -1 ) since diet substitution.…”

“…The half-life of carbon (T 50% ) in muscles at t = T necessary for initial atom substitution by the final atoms was determined by the equation: t = (-1 / k) ln (1 -F), in which t is the time of the initial atom substitution until the final substitution (days); ln is the Naperian logarithm (natural); F is the value of atom substitution, which can vary among 0.0 and 0.99; and k is the turnover constant (day -1 ) (Ducatti et al, 2014).…”