Turning waste to wealth-biodegradable plastics polyhydroxyalkanoates from palm oil mill effluent – a Malaysian perspective

Mumtaz, Tabassum; Yahaya, Noor Amalina; Abd‐Aziz, Suraini; Rahman, Nor'aini Abdul; Yee, Phang Lai; Shirai, Yoshihito; Hassan, Mohd Ali

doi:10.1016/j.jclepro.2010.05.016

Cited by 113 publications

(37 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pseudomonas can produce comparatively wider range of PHA on different wastes such as P(3HB:3HV) on whey [53], P(3HO:3HD:3HDD) on coprah oil [54], P(3HHx:3HO:3HD:3HDD:3HTD) on delignified rye grass hydrolysate [55] and P(3HHx:3HO:3HD:3HDD: 3HDDE:3HTD:3HTDE) on soy molasses [26]. As also observed with FAs, oily waste can provide a wider range of monomers in PHA [56,57]. By co-feeding soybean oil and c-butyrolactone as C sources, P(3HB:4HB) can be synthesized by R. eutropha KCTC2662 having 4HB fractions of 6-10 mol% [22].…”

Section: Substrate and Feeding Regimesupporting

confidence: 58%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

show abstract

Section: Substrate and Feeding Regimesupporting

confidence: 58%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The substrates mostly comprise organic matter, fat, sugar, carbohydrate and protein. An exception is glycerol, a by-product of the biodiesel process, which contains 80 85% glycerol with a trace amount of methanol (Mu et al, 2006;Mumtaz et al, 2010).…”

Section: Methodsmentioning

confidence: 99%

Screening and identification of polyhydroxyalkanoates producing bacteria and biochemical characterization of their possible application

Sangkharak

Prasertsan

2012

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs) accumulating bacteria were isolated under various selective conditions such as pH, salt concentrations and types of heavy metal. Fifty strains of bacterial isolates were found to belong to Bacillus, Proteus, Pseudomonas, Aeromonas, Alcaligenes and Chromobacterium, based on phenotypical features and genotypic investigation. Only twenty fi ve bacterial isolates were selected and observed for the production of PHAs. Interestingly, bacteria belonging to Firmucutes Bacillus sp. produced a high amount of PHAs. The maximum PHAs were accumulated by B. licheniformis PHA 007 at 68.80% of dry cell weight (DCW). Pseudomonas sp., Aeromonas sp., Alcaligenes sp. and Chromobacterium sp. were recorded to produce a moderate amount of PHAs, varying from 10.00 44.32% of DCW. The enzymatic activity was preliminarily analyzed by the ratio of the clear zone diameter to colony diameter. Bacillus gave the highest ratio of hydrolysis zone which corresponds to the highest hydrolytic enzyme activities. Bacillus licheniformis PHA 007 had the highest lipase and protease activity at 2.1 and 5.1, respectively. However, the highest amylase activity was observed in Bacillus sp. PHA 023 at 1.4. Determination of metabolic characteristics was also investigated to check for their ability to consume a wide range of substrates. Bacillus, Aeromonas sp. and Alcaligenes sp. had great ability to utilize a variety of substrates. To decrease high PHA cost, different sources of cheap substrates were tested for the production of PHAs. Bacillus cereus PHA 008 gave the maximal yield of PHA production (64.09% of DCW) when cultivated in anaerobically treated POME. In addition, the accumulation of PHA copolymers such as 3-hydroxyvalerate and 3-hydroxyhexanoate was also observed in Bacillus and Pseudomomas sp. strain 012 and 045, respectively. Eight of the nine isolates accumulated a signifi cant amount of PHAs when inexpensive carbon sources were used as substrates. Here it varied from 1.69% of DCW by B. licheniformis PHA 007 to 64.09% of DCW by B. cereus PHA 008.

show abstract

“…The positive progress of palm oil industry however comes with serious impact to the environment. The process of extracting crude palm oil from the fresh fruit bunch (FFB) requires an estimate of 1.5 m3 of water to process one tonne of FFB where half of the amount will end up as palm oil mill effluent (POME) [8]. POME is a colloidal suspension which consists of 95-96% water, 0.6-0.7% oil and 4-5% total solids.…”

Section: Introductionmentioning

confidence: 99%

Performance ofKlebsiella oxytocato generate electricity from POME in microbial fuel cell

Islam

Rahman

Yousuf

et al. 2016

MATEC Web of Conferences

View full text Add to dashboard Cite

Abstract. This study is aimed to evaluate the electricity generation from microbial fuel cell (MFC) and to analyze the microbial community structure of city wastewater and anaerobic sludge to enhance the MFC performance. MFCs, enriched with palm oil mill effluent (POME) were employed to harvest electricity by innoculating of Klebsiella oxytoca, collected from city wastewater and other microbes from anaerobic sludge (AS). The MFC showed maximum power density of 207.28 mW/m 3 with continuous feeding of POME using microbes from AS. Subsequent replacement with Klebsiella oxytoca resulted maximum power density of 1236 mW/m 3 by utilizing complex substrate POME which was six times higher as compared to MFC operated with AS. Based on Biolog gene III analysis, relatively higher abundance of Klebsiella oxytoca was detected in the city wastewater. Predominant microorganisms such as Gammaproteobacteria, Azospiraoryzae, Acetobacterperoxydans and Solimonasvariicoloris were isolated from palm oil anaerobic sludge as well as from biofilm of MFC. Enriched electrochemically active bacteria Klebsiella oxytoca showed better performance to generate electricity from complex POME substrates compare to AS. These results demonstrate that the power output of MFCs can be increased significantly using Klebsiella oxytoca.

show abstract

Turning waste to wealth-biodegradable plastics polyhydroxyalkanoates from palm oil mill effluent – a Malaysian perspective

Cited by 113 publications

References 74 publications

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Screening and identification of polyhydroxyalkanoates producing bacteria and biochemical characterization of their possible application

Performance ofKlebsiella oxytocato generate electricity from POME in microbial fuel cell

Contact Info

Product

Resources

About