Turning heads: Development of vertebrate branchiomotor neurons

Chandrasekhar, Anand

doi:10.1002/dvdy.10444

Cited by 164 publications

(209 citation statements)

References 202 publications

Supporting

Mentioning

204

Contrasting

Order By: Relevance

“…For example, motor neuron cell bodies of cranial nerve V (nV) arise in rhombomere (r)2 and r3. This well-defined patterning of nV motor neuron cell bodies is conserved among mouse, chick, and zebrafish (Chandrasekhar, 2004). Establishment and maintenance of specific rhombomere borders is partially determined by the Hox gene family (Lumsden and Krumlauf, 1996;Moens and Prince, 2002).…”

Section: Introductionmentioning

confidence: 98%

Evolutionarily conserved function of Gbx2 in anterior hindbrain development

et al. 2011

Self Cite

View full text Add to dashboard Cite

The amino acid sequence across the DNA-binding homeodomain of Gbx2 is highly conserved across multiple species. In mice, Gbx2 is essential for establishment of the midbrain-hindbrain boundary (MHB), and in development of anterior hindbrain structures, rhombomeres (r) 1-r3, and the r2/r3-derived cranial nerve V. In contrast, studies in zebrafish have implicated gbx1 in establishment of the MHB. Therefore, we tested potential roles for gbx2 in anterior hindbrain development in zebrafish. gbx2 knockdown with antisense morpholino results in increased cell death in r2, r3, and r5 and a truncation of the anterior hindbrain, similar to the defect in Gbx2 2/2 mice. Moreover, there is abnormal clustering of cranial nerve V cell bodies in r2 and r3 indicative of defects in aspects of anterior hindbrain patterning. These phenotypes can be rescued by expression of the mouse GBX2 protein. These results suggest that gbx2/Gbx2 has an evolutionarily conserved role in anterior hindbrain development. Developmental Dynamics 240:828-838,

show abstract

Section: Introductionmentioning

confidence: 98%

Evolutionarily conserved function of Gbx2 in anterior hindbrain development

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the zebrafish (Danio rerio), FBMNs are born beginning at 16 hours post-fertilization (hpf) in rhombomere (r) 4. Shortly after birth, these neurons undergo caudal migration to r6 and r7 (for reviews, see Chandrasekhar, 2004;Song, 2007). Among many proteins known to regulate this migration in zebrafish are several core planar cell polarity (PCP) components (reviewed by Wada and Okamoto, 2009a;Wada and Okamoto, 2009b).…”

Section: Introductionmentioning

confidence: 99%

Zebrafish Prickle1b mediates facial branchiomotor neuron migration via a farnesylation-dependent nuclear activity

et al. 2011

View full text Add to dashboard Cite

SUMMARYThe facial branchiomotor neurons (FBMNs) undergo a characteristic tangential migration in the vertebrate hindbrain. We previously used a morpholino knockdown approach to reveal that zebrafish prickle1b (pk1b) is required for this migration. Here we report that FBMN migration is also blocked in a pk1b mutant with a disruption in the consensus farnesylation motif. We confirmed that this lipid modification is required during FBMN migration by disrupting the function of farnesyl biosynthetic enzymes. Furthermore, farnesylation of a tagged Pk1b is required for its nuclear localization. Using a unique rescue approach, we have demonstrated that Pk1b nuclear localization and farnesylation are required during FBMN migration. Our data suggest that Pk1b acts at least partially independently of core planar cell polarity molecules at the plasma membrane, and might instead be acting at the nucleus. We also found that the neuronal transcriptional silencer REST is necessary for FBMN migration, and we provide evidence that interaction between Pk1b and REST is required during this process. Finally, we demonstrate that REST protein, which is normally localized in the nuclei of migrating FBMNs, is depleted from the nuclei of Pk1b-deficient neurons. We conclude that farnesylation-dependent nuclear localization of Pk1b is required to regulate REST localization and thus FBMN migration.

show abstract

“…A number of studies have addressed the migration of hindbrain neurons and several genes affecting their migration have been identified (reviewed by Hatten, 1999;Wingate 2001;Chandrasekhar, 2004;Sotelo, 2004). Yet, cellular basis for nucleogenesis is still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Direct visualization of nucleogenesis by precerebellar neurons:involvement of ventricle-directed, radial fibre-associated migration

et al. 2006

View full text Add to dashboard Cite

Nuclei are aggregates of neurons distributed in the central nervous system and are fundamental functional units that share anatomical and physiological features. Despite their importance, the cellular basis that leads to nucleogenesis is only poorly understood. Using exo utero electroporation with an enhanced yellow fluorescent protein (EYFP) gene, we show that the precerebellar neurons derived from the lower rhombic lip (lRL) undergo multiple migration steps to form nuclei. After the unilateral transfer of EYFP to the lRL of embryonic day 12.5 mice, EYFP-labelled neurons migrate tangentially from the lRL in two distinct streams, one towards the ventral metencephalon and the other towards the ventral myelencephalon. These neurons cross the ventral midline and then become radially directed. Labelled neurons in the tangential migratory streams form contralateral clusters in the external cuneate nucleus (ECN) and lateral reticular nucleus (LRN) in the myelencephalon, and bilateral clusters in the pontine grey nucleus (PGN) and reticulotegmental nucleus (RTN) in the metencephalon. Before forming the clusters, EYFP-labelled neurons begin to migrate radially towards the ventricle in close apposition to nestin-positive radial fibres, and then they aggregate as they detach from the fibres. Inhibition of cadherin function in ECN and LRN progenitors caused ipsilateral formation of the ECN and LRN,implying that the transition of their migration from tangential to radial involves a cell-intrinsic mechanism. These observations suggest that nucleogenesis of precerebellar neurons is a result of multi-phasic migration,and that ventricle-directed radial glia-guided migration is a key step for nucleogenesis.

show abstract

Turning heads: Development of vertebrate branchiomotor neurons

Cited by 164 publications

References 202 publications

Evolutionarily conserved function of Gbx2 in anterior hindbrain development

Evolutionarily conserved function of Gbx2 in anterior hindbrain development

Zebrafish Prickle1b mediates facial branchiomotor neuron migration via a farnesylation-dependent nuclear activity

Direct visualization of nucleogenesis by precerebellar neurons:involvement of ventricle-directed, radial fibre-associated migration

Contact Info

Product

Resources

About