Turning gray selenium and sublimed sulfur into a nanocomposite to accelerate tissue regeneration by isothermal recrystallization

Abstract

Background Globally, millions of patients suffer from regenerative deficiencies, such as refractory wound healing, which is characterized by excessive inflammation and abnormal angiogenesis. Growth factors and stem cells are currently employed to accelerate tissue repair and regeneration; however, they are complex and costly. Thus, the exploration of new regeneration accelerators is of considerable medical interest. This study developed a plain nanoparticle that accelerates tissue regeneration with the involvement of angiogenesis and inflammatory regulation. Methods Grey selenium and sublimed sulphur were thermalized in PEG-200 and isothermally recrystallised to composite nanoparticles (Nano-Se@S). The tissue regeneration accelerating activities of Nano-Se@S were evaluated in mice, zebrafish, chick embryos, and human cells. Transcriptomic analysis was performed to investigate the potential mechanisms involved during tissue regeneration. Results Through the cooperation of sulphur, which is inert to tissue regeneration, Nano-Se@S demonstrated improved tissue regeneration acceleration activity compared to Nano-Se. Transcriptome analysis revealed that Nano-Se@S improved biosynthesis and ROS scavenging but suppressed inflammation. The ROS scavenging and angiogenesis-promoting activities of Nano-Se@S were further confirmed in transgenic zebrafish and chick embryos. Interestingly, we found that Nano-Se@S recruits leukocytes to the wound surface at the early stage of regeneration, which contributes to sterilization during regeneration. Conclusion Our study highlights Nano-Se@S as a tissue regeneration accelerator, and Nano-Se@S may provide new inspiration for therapeutics for regenerative-deficient diseases. Graphical Abstract

SAIF plays anti-angiogenesis via blocking VEGF-VEGFR2-ERK signal in tumor treatment

“…Wound healing experiment was performed as previously described [ 26 ]. HUVEC cells (1 × 10 5 cells per well) were inoculated in 6-well plates and incubated at 37 °C, 5% CO 2 for 24 h. After forming a monolayer of cells, they were treated with DMEM (0.5% FBS) for 24 h to starve them.…”

“…The detailed protocol was described in previous study [ 26 ]. HUVEC cells were lysed on ice by TRIZOL.…”

GSH-responsive degradable nanodrug for glucose metabolism intervention and induction of ferroptosis to enhance magnetothermal anti-tumor therapy

“…Encouraged by the magnetic properties of LFMP in vitro, the synergistic therapeutic performance of LFMP was systematically evaluated. It is widely recognized that low toxicity is essential for future applications as a biological nanomaterial and medication carrier [38,39]. Therefore, the toxicity of LFMP was measured and analyzed through the CCK-8 experiment.…”

The Complementary Role of Selenium and Its Botanical Combinations in Regenerative Therapy

“…The regeneration of tissues has complex physiological procedures such as cell growth, angiogenesis, inflammatory responses, remodeling of tissues, homeostasis, vascularization, and redox reactions. (Cao et al, 2023). Mesenchymal stem cells (MSCs) could be obtained from the placenta, adipose tissue, blood, and bone marrow.…”