Turning Coacervates into Biohybrid Glass: Core/Shell Capsules Formed by Silica Precipitation in Protein/Polysaccharide Scaffolds

Erni, Philipp; Dardelle, Gregory; Sillick, Matthew; Davidson, T.N.; Beaussoubre, Pascal; Fieber, Wolfgang

doi:10.1002/ange.201303489

Cited by 7 publications

(6 citation statements)

References 53 publications

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“…Coacervate micro-droplets are produced by liquid–liquid phase separation and exhibit a range of biomimetic properties such as selective molecular uptake, 7 , 8 micro-compartmentalized nanoparticle 9 or enzyme catalysis, 10 in vitro gene expression, 11 , 12 and templating of lipid membrane multilayer assembly. 13 Although recent studies have used auxiliary components such as inorganic nanoparticles, 14 inorganic polyanionic clusters, 15 covalent crosslinking, 16 , 17 and hydrogels 18 , 19 to produce higher-order coacervate-based micro-architectures, the use of coacervate micro-droplets as an intrinsic, structurally reconfigurable micro-compartmentalized phase has been rarely exploited. 20 Coacervates based on polymer/small molecule (monomer) complexation show considerable promise as reconfigurable protocells because the relative weakness of the electrostatic interactions increases the scope to structurally and compositionally manipulate the micro-droplets by environmental triggers such as changes in salt concentration, pH and temperature.…”

Section: Introductionmentioning

confidence: 99%

Self-transformation and structural reconfiguration in coacervate-based protocells

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Self-transformation and structural reconfiguration in coacervate-based protocells

et al. 2016

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“…Erni et al synthesized microcapsules with dense walls composed entirely of a biopolymer scaffold interpenetrated with amorphous silica. [ 37 ] A weakly acidic hydrogel shell was first formed around oil droplets, which then served as a scaffold to induce protein‐directed mineralization of silica. The precipitation process, occurring in the hydrogel scaffold, consumed water to form silica, yielding dense shells with a very low permeability for volatile organic compounds.…”

Section: Introductionmentioning

confidence: 99%

Versatile Preparation of Silica Nanocapsules for Biomedical Applications

Jiang

Mottola

Han

et al. 2020

Part & Part Syst Charact

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Core–shell nanocapsules are receiving increasing interest for drug delivery applications. Silica nanocapsules have been the focus of intensive studies due to their biocompatibility, versatile silica chemistry, and tunable porosity. However, a versatile one‐step preparation of silica nanocapsules with well‐defined core–shell structure, tunable size, flexible interior loading, and tailored shell composition, permeability, and surface functionalization for site‐specific drug release and therapeutic tracking remains a challenge. Herein, an interfacially confined sol–gel process in miniemulsion for the one‐step versatile preparation of functional silica nanocapsules is developed. Uniform nanocapsules with diameters from 60 to 400 nm are obtained and a large variety of hydrophobic liquids are encapsulated in the core. When solvents with low boiling point are loaded, subsequent solvent evaporation converts the initially hydrophobic cavity into an aqueous environment. Stimuli‐responsive permeability of nanocapsules is programmed by introducing disulfide or tetrasulfide bonds in the shell. Selective and sustained release of dexamethasone in response to glutathione tripeptide for over 10 d is achieved. Fluorescence labeling of the silica shell and magnetic loading in the internal cavity enable therapeutic tracking of nanocapsules by fluorescence and electron microscopies. Thus, silica nanocapsules represent a promising theranostic nanoplatform for targeted drug delivery applications.

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“…Inspired by nature, biomimetic templating methods have emerged as relatively new approaches to synthesize silica-based nanocarriers owing to the abilities of mineralizing biomolecules to recognize, interact with, and direct the nucleation and growth of silica under physiological conditions. − For example, Jakhmola et al synthesized oil-core silica-shell nanocapsules, thereby using a layer-by-layer assembly method in which oppositely charged biomolecules were alternately deposited on a nanoemulsion template followed by silica mineralization . Erni et al reported the synthesis of silica capsules having a diameter at a scale of hundreds of micrometers by silicification of cross-linked coacervate of gelatin and Acacia gum around oil droplets …”

Section: Introductionmentioning

confidence: 99%

Biomimetic Silica Nanocapsules for Tunable Sustained Release and Cargo Protection

et al. 2017

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Silica nanocapsules have attracted tremendous interest for encapsulation, protection, and controlled release of various cargoes due to their unique hierarchical core-shell structure. However, it remains challenging to synthesize silica nanocapsules having high cargo-loading capacity and cargo-protection capability without compromising process simplicity and biocompatibility properties. Here, we synthesized oil-core silica-shell nanocapsules under environmentally friendly conditions by a novel emulsion and biomimetic dual-templating approach using a dual-functional protein, in lieu of petrochemical surfactants, thus avoiding the necessities for the removal of toxic components. A light- and pH-sensitive compound can be facilely encapsulated in the silica nanocapsules with the encapsulation efficiency of nearly 100%. Release of the encapsulated active from the nanocapsules was not shown an indication of undesired burst release. Instead, the release can be tuned by controlling the silica-shell thicknesses (i.e., 40 and 77 nm from which the cargo released at 42.0 and 31.3% of the initial amount after 32 days, respectively). The release kinetics were fitted well to the Higuchi model, enabling the possibility of the prediction of release kinetics as a function of shell thickness, thus achieving design-for-purpose silica nanocapsules. Furthermore, the nanocapsules showed excellent alkaline- and sunlight-shielding protective efficacies, which resulted in significantly prolonged half-life of the sensitive cargo. Our biomimetic silica nanocapsules provide a nanocarrier platform for applications that demand process scalability, sustainability, and biocompatibility coupled with unique cargo-protection and controlled-release properties.

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Turning Coacervates into Biohybrid Glass: Core/Shell Capsules Formed by Silica Precipitation in Protein/Polysaccharide Scaffolds

Cited by 7 publications

References 53 publications

Self-transformation and structural reconfiguration in coacervate-based protocells

Self-transformation and structural reconfiguration in coacervate-based protocells

Versatile Preparation of Silica Nanocapsules for Biomedical Applications

Biomimetic Silica Nanocapsules for Tunable Sustained Release and Cargo Protection

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