Turn-on silicon-based fluorescent probe for visualizing endogenous CO during hypoxia

Gai, Fengqing; Guo, Xuewen; Ding, Guowei; Zhang, Kun; Zhang, Yafang; Zuo, Yujing

doi:10.1039/d2nj01696f

Cited by 4 publications

(2 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescent biological imaging is considered one of the most commonly used detection methods due to its high sensitivity, selectivity, and versatility. − Encouraged by the previously mentioned promising results of the sensing platform for solution-based CO detection, we evaluated the ability of the TPANN-Pd system for the “turn on” sensing of endogenous CO by cell-imaging in live Hela (human cervical cancer cells) utilizing SP8 confocal laser scanning microscopy. For potential bioimaging application in living cells, the cytotoxicity of the sensing platform was first explored with standard MTT assay with various concentrations from 1 to 20 μM of TPANN-Pd for 24 h (Figure S20).…”

Section: Resultsmentioning

confidence: 99%

High-Performance Recognition, Cell-Imaging, and Efficient Removal of Carbon Monoxide toward a Palladium-Mediated Fluorescent Sensing Platform

Fang

Zhang

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

Novel high-performance fluorescent approaches have always significant demand for room-temperature detection of carbon monoxide (CO), which is highly toxic even at low concentration levels and is not easy to recognize due to its colorless and odorless nature. In this paper, we constructed a palladium-mediated fluorescence turn-on sensing platform (TPANN-Pd) for the recognition of CO at room temperature, revealing simultaneously quick response speed (<30 s), excellent selectivity, superior sensitivity, and low detection limit (∼160 nM for CORM-3, ∼1.7 ppb for CO vapor). Moreover, rapid detection and efficient removal (24%) from the air by naked-eye vision has been successfully realized based on TPANN-Pd supramolecular gels. Furthermore, the developed sensing platform was elucidated with low cytotoxicity and high cellular uptake, and it was successfully applied to CO imaging in living cells, providing real-time monitoring of potential CO-involved reactions in biological systems.

show abstract

Section: Resultsmentioning

confidence: 99%

High-Performance Recognition, Cell-Imaging, and Efficient Removal of Carbon Monoxide toward a Palladium-Mediated Fluorescent Sensing Platform

Fang

Zhang

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…[31][32][33][34][35][36][37][38][39][40] However, the existing CO probes still have some shortcomings, such as low sensitivity, poor water solubility, and complex structure, which limit their further use (Table S1, ESI †). [41][42][43] Undoubtedly, it is very significant to develop CO fluorescent probes with higher sensitivity and better selectivity for the detection of CO in environmental and biological samples.…”

Section: Introductionmentioning

confidence: 99%

A novel highly sensitive fluorescent probe for imaging endogenous CO

et al. 2022

View full text Add to dashboard Cite

show abstract

Recent Progress in CO Detection Probes Based on Different Fluorophores

Fang,

Jiang,

et al. 2024

Analysis & Sensing

View full text Add to dashboard Cite

Carbon monoxide (CO), a simple and well‐known toxic gas, is a naturally occurring gaseous transmitter that plays a crucial role in the regulation of physiological and pathological processes in living organisms. Usually, the development of various diseases can lead to the dysregulation of CO levels. Interestingly, CO has been shown to exert therapeutic effects in inflammation‐related disease models. Fluorescent probes for CO detection have become a vital research field in the past decades owing to their advantages of excellent selectivity, exceptional sensitivity, and real‐time in situ detection, which have been employed for the precise detection of CO in cells, tissues, and even living organisms. This paper reviews research advancements in CO fluorescent probes over the last decade, outlines the design concepts and detection mechanisms of relevant fluorescent probes, and provides design guidelines and future development prospects.

show abstract

Turn-on silicon-based fluorescent probe for visualizing endogenous CO during hypoxia

Abstract: Endogenous carbon monoxide (CO) is mainly derived from degraded heme owing to HO-1 expression, which acts as a pivotal part in vasodilation and neurotransmitter, anti-inflammatory and anti-apoptosis process. Due to...

Cited by 4 publications

References 50 publications

High-Performance Recognition, Cell-Imaging, and Efficient Removal of Carbon Monoxide toward a Palladium-Mediated Fluorescent Sensing Platform

High-Performance Recognition, Cell-Imaging, and Efficient Removal of Carbon Monoxide toward a Palladium-Mediated Fluorescent Sensing Platform

A novel highly sensitive fluorescent probe for imaging endogenous CO

Recent Progress in CO Detection Probes Based on Different Fluorophores

Contact Info

Product

Resources

About