“Turn-on” FRET-based luminescent iridium(iii) probes for the detection of cysteine and homocysteine

Shiu, Hoi Yan; Wong, Man Leung; Ming, Chi

doi:10.1039/c0cc04288a

Cited by 85 publications

(34 citation statements)

References 33 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More recently, Ru complexes have played the role of both acceptors and donors in a three-color FRET relay for studying DNA and polypeptide dynamics and interactions [412,413]. Os(II) complexes have been coupled as acceptors in FRET studies to QD donors [414,415], and Ir(III) FRET probes have been developed for cysteine and homocysteine detection [416]. A Zn porphyrin cofactor in Zn(II)-substituted horse heart cytochrome c was shown to serve as a donor to a Alex660 acceptor in cytochrome c unfolding studies [417].…”

Section: Luminescent Transition Metal Complexesmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

View full text Add to dashboard Cite

The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

show abstract

Section: Luminescent Transition Metal Complexesmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

View full text Add to dashboard Cite

show abstract

“…Because of the important roles of thiols in biological systems, there is a rapidly growing interest in the determination of biothiols (Gong et al, 2005;Lee et al, 2008;Lu et al, 2006;Ros-Lis et al, 2004). Up to now, many methods have been established for the quantitative analysis of these biothiols including mass spectroscopy (Burford et al, 2003;Johnson et al, 2008), chromatography (Lu et al, 2007;Nolin et al, 2007;Yang et al, 2009), fluorescence methods (Choi et al, 2009;Ran et al, 2013;Shiu et al, 2011;Wu et al, 2014;Zhang et al, 2014), electrochemical assay etc (Agüí et al, 2007;Lawrence et al, 2004). Although these methods can be used for the detection of exceedingly low level of biothiols, some inherent issues still cannot be avoided, such as complex operations, costly polymerase, tedious labels, and dedicated instrumentation.…”

Section: Introductionmentioning

confidence: 98%

Label-free colorimetric detection of biothiols utilizing SAM and unmodified Au nanoparticles

Zheng

Zhang

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…As a well-known thiol-containing amino acid in this family, Cys serves as the building block in protein synthesis, detoxification, and metabolism in live organisms Chen et al, 2013;Chen et al 2013;Li et al, 2009;Das et al, 2012). Alterations in the level of cellular Cys has been implicated with neurotoxicity, and the deficiency of Cys may lead to many syndromes, such as a hematopoiesis decrease, slow growth, hair depigmentation, leucocyte loss, psoriasis and so on (Shiu et al, 2011;Yin et al, 2013;Shao et al, 2011;Zhou et al, 2013;Wang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%