“Turn-on” fluorometric probe for α-glucosidase activity using red fluorescent carbon dots and 3,3′,5,5′-tetramethylbenzidine

Liu, Jinshui; Wu, Fangfei; Liu, Chenfu; Bao, Huijuan; Fu, Ting

doi:10.1007/s00604-020-04479-1

Cited by 22 publications

(10 citation statements)

References 35 publications

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“…α-Glu solutions (100 μL) with three different levels (0.5, 1, and 1.5 U/mL) were then added into 100 μL of diluted human serum sample solutions. Later, fluorometric and colorimetric assays of the prepared samples were performed following the steps described in the section on the α-Glu assay. , …”

Section: Methodsmentioning

confidence: 99%

“…Later, fluorometric and colorimetric assays of the prepared samples were performed following the steps described in the section on the α-Glu assay. 1,8 3. RESULTS AND DISCUSSION 3.1.…”

Section: Synthesis and Purification Of Si Cnpsmentioning

confidence: 99%

“…α-Glucosidase is a type of enzyme playing a crucial role in the digestion of carbohydrates. , It is a membrane-attached enzyme mainly found at the epithelium of the small intestine . Its main activity is to hydrolyze α-glucopyranoside bonds and hence discharge α-glucose from the non-reducing end of sugar .…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, designing straightforward multichannel optical probes for α-Glu detection with high sensitivity is still desirable. Moreover, to the best of our knowledge, most of the reports on the α-Glu activity assay use l -ascorbic acid-2-O-α- d -glucopyranosyl (AAG), , 4-nitrophenyl-α- d -glucopyranoside (NGP), and 4-aminophenyl-α- d -glucopyranoside (pAPG) , as α-Glu substrates. However, such available α-Glu substrates (AAG, NGP, and pAPG) have been extensively used; meanwhile, discovering novel and preferable α-Glu substrates for rapid and moderate optical signals is still desirable.…”

Section: Introductionmentioning

confidence: 99%

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α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

et al. 2021

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Designing analytical approaches for enzymatic activity monitoring with high sensitivity and selectivity is of critical value for the diagnosis of diseases and biomedical studies. In this study, we have created a facile one-step synthetic route to prepare orange-red color and yellow fluorescent silicon-containing nanoparticles (Si CNPs) by mixing 3(2-aminoethylamino) propyl (dimethoxymethylsilane) and hydroquinone (HQ) in an aqueous solution. Inspired by the HQ-regulated facile synthetic step and the generation of HQ from α-glucosidase (α-Glu)-catalyzed hydrolysis of 4hydroxyphenyl-α-D-glucopyranosyl (4-HPαDG), we have designed a straightforward colorimetric and fluorometric α-Glu activity assay using a commercially available 4-HPαDG as the α-Glu substrate. Fluorescent and colorimetric assays for α-Glu activity measurement have been thereby established and exhibited detection limits as low as 0.0032 and 0.0046 U/mL, respectively. Under single excitation at 370 nm, the prepared Si CNPs emitted yellow fluorescence at 520 nm and exhibited an absorbance peak at 390 nm. In addition, the proposed approach reveals various advantages including easy operation, time-saving, and good anti-interference ability. Hence, it could improve the progress of fluorometric and colorimetric enzymatic activity assays with high sensitivity and simplicity. Moreover, the proposed approach was applied for α-Glu inhibitor screening, and its feasibility in real samples was measured by detecting the α-Glu activity in human serum samples.

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Section: Methodsmentioning

confidence: 99%

“…Later, fluorometric and colorimetric assays of the prepared samples were performed following the steps described in the section on the α-Glu assay. 1,8 3. RESULTS AND DISCUSSION 3.1.…”

Section: Synthesis and Purification Of Si Cnpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

et al. 2021

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“…Carbon dots (CDs) exhibit outstanding photoluminescence (PL) performances, high photostability against photobleaching, excellent solubility, low toxicity and good biocompatibility, which make them appropriate for use in in vivo imaging, 1,2 optoelectronic devices, 3,4 and fluorescence sensing. 5–9 CDs have been considered as a substitute for traditional heavy metal-based semiconductor quantum dots because they are environmentally friendly. 10 For many practical applications, it is strongly required to prepare CDs with full-color-emission.…”

Section: Introductionmentioning

confidence: 99%

Preparation of multicolor-emissive carbon dots with high quantum yields and their epoxy composites for fluorescence anti-counterfeiting and light-emitting devices

et al. 2022

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Ratiometric fluorescence nanoprobe based on carbon dots and terephthalic acid for determining Fe2+ in environmental samples

Wang

et al. 2022

Anal Bioanal Chem

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“Turn-on” fluorometric probe for α-glucosidase activity using red fluorescent carbon dots and 3,3′,5,5′-tetramethylbenzidine

Cited by 22 publications

References 35 publications

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

Preparation of multicolor-emissive carbon dots with high quantum yields and their epoxy composites for fluorescence anti-counterfeiting and light-emitting devices

Ratiometric fluorescence nanoprobe based on carbon dots and terephthalic acid for determining Fe2+ in environmental samples

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