Turn-on fluorescent probe for sensing exogenous and endogenous hypochlorous acid in living cells, zebrafishes and mice

Real-time optical imaging of the hypoxic status in hemangioma endothelial cells during propranolol therapy

“…We tried to further identify the response strategy of HNT-NTR. Zebrafish was a prominent vertebrate model which was widely applied in the detection of drug delivery and fluorescent probe ( 30 , 31 ), due to its features such as body transparency, simple and rapid construction, and small size ( 31 ). We incubated HNT-NTR with zebrafish in 3 different groups (dicoumarin group, probe group and blank control group) to identify the visualization ability in vivo .…”

An ICT-FRET-based fluorescent probe for the ratiometric sensing of hypochlorous acid based on a coumarin–naphthalimide derivative

“…[20][21][22] Up to now, numerous fluorescent probes have been developed and applied to the detection of hypochlorous acid. [23][24][25][26][27][28][29][30] Most of these fluorescent probes respond only at a single wavelength, and the fluorescence intensity changes with the concentration of the target analyte, which is easily affected by many factors, such as instrument, environment, probe concentration, photobleaching and excitation intensity. [31][32][33] The ratiometric probes can overcome these shortcomings.…”

A benzocoumarin-based fluorescent probe for ultra-sensitive and fast detection of endogenous/exogenous hypochlorous acid and its applications

“…5,6 This innate immune weapon can resist the invasion of bacteria and pathogens, suppress inflammation, fight pathogens, and regulate cellular apoptosis in various physiological and pathological processes. [7][8][9][10][11][12] In addition, fluctuations of HOCl in organisms are closely related to inflammation and relevant diseases and can be used as critical diagnostic signaling molecules. [13][14][15][16] Natural HOCl is mainly generated from H 2 O 2 and Cl − catalyzed by myeloperoxidase (MPO, an enzyme in immune cells).…”