Turn-On, Fluorescent Nuclear Stains with Live Cell Compatibility

Pitter, Demar R. G.; Wigenius, Jens; Brown, Adrienne S.; Baker, James D.; Westerlund, Fredrik; Wilson, James N.

doi:10.1021/ol400268t

Cited by 26 publications

(27 citation statements)

References 17 publications

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“…Nucleic acid-staining uorophores have been extensively used in biological research and medical diagnosis, including cellular imaging and DNA quantication. [1][2][3][4][5][6][7][8][9][10][11] Although many such probes are available, only a few of them, such as Hoechst and DRAQ5, are suitable for live-cell imaging. Hoechst is the most commonly used probe, but it must be excited by UV illumination, which is known to damage cellular DNA.…”

Section: Introductionmentioning

confidence: 99%

A bright red fluorescent cyanine dye for live-cell nucleic acid imaging, with high photostability and a large Stokes shift

Zhang

Fan

et al. 2014

J. Mater. Chem. B

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Many probes for nucleic acids are available, but few of them satisfy multiple criteria, particularly high photostability to endure laser scanning. We report a cyanine dye TO3-CN for the first time, synthesised by introducing a CN group to the trimethine chain of the classical red emitting TO-3 dye to improve its photostability, as well as their spectral properties and interaction with nucleic acids. TO3-CN shows excellent light fastness and a large fluorescence Stokes shift (more than 40 nm). Because of its sensitive fluorescence response to nucleic acids with a large fluorescence quantum yield (more than 0.7) and low cytotoxicity, this dye may be a potential candidate for nucleic acid detection in vitro and intracellular fluorescence imaging.

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Section: Introductionmentioning

confidence: 99%

A bright red fluorescent cyanine dye for live-cell nucleic acid imaging, with high photostability and a large Stokes shift

Zhang

Fan

et al. 2014

J. Mater. Chem. B

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“…FIGURE 1(a) depicts a molecular complex consisting a double stranded DNA (dsDNA), a turn-on fluorescent probe, and a drug to be delivered. The probe, which operates based on the turn-on effect, show significant fluorescence as it is bound to DNA initially [6]. Due to enzymatic digestion in target organelles, as shown in FIGURE 1 (b), the dsDNA unwinds and splits into pieces, which results in the detachment of the probe and drug [7].…”

Section: Introductionmentioning

confidence: 99%

Fully Integrated Liquid-Core Waveguide Fluorescence Lifetime Detection Microsystem for DNA Biosensing

et al. 2019

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Time-resolved fluorescence is a widely adopted technique for DNA detection due to its high sensitivity and selectivity. However, due to stringent requirements on optics and electronics, instrumentation with time-resolved capability is bulky and expensive, prohibiting their use in portable and point-of-care applications. In this work, a fully-integrated DNA biosensor based on liquid-core waveguide (LCW) optics for fluorescence lifetime analysis is presented. The DNA biosensor encompasses all-custom bioassay, optics and electronics into a microsystem, delivering a near sample-to-answer level of integration. Lifetime, rather than intensity, is exploited as the analytical signal from the V-carbazole probe for the first time. Excitation propagation within the LCW is investigated both analytically and in simulations to achieve high excitation rejection and low temporal dispersion, enabling the proposed LCW-based system with much smaller instrumentation size to deliver comparable lifetime measurement accuracy to traditional systems. Detection of DNA down to 15 base pairs at a low detection limit of 1.38 nM demonstrates the high applicability of the proposed biosensor for compact, application-specific, and low-cost diagnostics devices.INDEX TERMS DNA biosensing, fluorescence lifetime detection, liquid-core waveguide, TCSPC, turn-on fluorescent probe.

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“…20 So far, a number of two-photon probes have been studied for cellular DNA or other organelles targeting and imaging. [21][22][23][24][25] However, studies involving organic small-molecule probes targeting cellular nucleus for the long-term monitoring and tracking are rarely reported. 26,27 Thus, extreme attention should be paid to nucleus monitoring for the enhancement of photostability in biomedical imaging.…”

Section: Introductionmentioning

confidence: 99%

Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging

Chi

2016

J. Phys. Chem. C

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A series of fluorenone-based two-photon fluorescent probes with high photostability for nucleus imaging are prepared and developed. The one-and two-photon photophysical properties exhibit these new probes possess 0.448−0.634 of fluorescence quantum yields and 469−495 GM of two-photon absorption cross sections at 800 nm femtosecond laser excitation. The luminescence "turn-on" experiment in buffer solutions indicates that 35-fold of fluorescence intensity and 68-fold fluorescence quantum yield enhancement appear between new probes and calf thymus DNA. In the nuclear doublestaining experiment, the high mean colocalization coefficients of 0.92−0.96 between new probes and nuclear labeling dye Hoechst 33342 are acquired, demonstrating excellent nuclear localization in 3T3 cells. The counterstain studies by introducing commercial mitochondrial staining reagent MTR and nuclear staining dye DAPI further show good membrane permeability and counterstain compatibility in multicolor cell labeling application. The photostability studies show that 3000 s of observation time and 0.028%/ s−0.03%/s of mean fluorescence attenuation rates under persistent laser irradiation in two-photon nuclear imaging are achieved. Meanwhile, the cause of fluorescence attenuation in the photostability test for cellular nuclei monitoring are discussed as well.

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Turn-On, Fluorescent Nuclear Stains with Live Cell Compatibility

Cited by 26 publications

References 17 publications

A bright red fluorescent cyanine dye for live-cell nucleic acid imaging, with high photostability and a large Stokes shift

A bright red fluorescent cyanine dye for live-cell nucleic acid imaging, with high photostability and a large Stokes shift

Fully Integrated Liquid-Core Waveguide Fluorescence Lifetime Detection Microsystem for DNA Biosensing

Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging

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