“Turn-on” Fluorescent Aptasensor Based on AIEgen Labeling for the Localization of IFN-γ in Live Cells

Ma, Ke; Zhang, Fengli; Sayyadi, Nima; Chen, Wenjie; Anwer, Ayad G.; Care, Andrew; Xu, Bin; Tian, Wenjing; Goldys, Ewa M.; Liu, Guozhen

doi:10.1021/acssensors.7b00720

Cited by 52 publications

(43 citation statements)

References 39 publications

(69 reference statements)

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“…Imaging was achieved through specific aptamer‐mediated binding with the overexpressed immunoglobulin mu heavy chain on the cell membrane of cancer cells . Another AIEgen–aptamer conjugate has been successfully used in the localization imaging of IFN‐γ (Figure and Table , entry 18) . It gave a LOD of 2 pg mL −1 in living cells, which is among the best intracellular IFN‐γ sensor reported to date.…”

Section: Detection Localization and Quantification Of Proteinsmentioning

confidence: 99%

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Xie

Wong

Chen

et al. 2019

Chemistry A European J

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Protein is one of the four most important biomacromolecules in living systems. The detection, quantification, localization, and characterization of proteins is essential for an understanding of biological fundamentals, as well as for the diagnostics and treatment of protein‐related diseases. By using intrinsic and extrinsic fluorescence, different techniques have been established to study proteins, many of which are now being routinely used in research laboratories and clinics. This review summarizes the applications of aggregation‐induced emission (AIE) fluorescence in protein science. In contrast to traditional fluorescent dyes, the activation of AIE dyes is mainly attributed to the restriction of intramolecular motions. This unique turn‐on mechanism of AIE dyes allows researchers to develop novel fluorogenic strategies for sensitive, selective, and reliable analysis of proteins. This review focuses on introducing AIE strategies for 1) detection, localization, and quantification of proteins; 2) probing polymer conformational transitions of proteins; 3) characterization of protein–ligand interactions; and 4) evaluation of enzyme activities. Perspectives and challenges with respect to this emerging field of protein characterization are offered.

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Section: Detection Localization and Quantification Of Proteinsmentioning

confidence: 99%

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Xie

Wong

Chen

et al. 2019

Chemistry A European J

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“…The other nucleolin is overexpressed on the surface of several cancer cell lines. To improve the analysis technology for detecting IFN‐γ, Tian and co‐workers reported an aptamer‐functionalized AIEgen TPE‐aptamer for detecting intracellular IFN‐γ. The TPE‐aptamer can detect the intracellular secretion IFN‐γ in vitro and its detection limit is 2 pg mL −1 .…”

Section: Key Application In Biomedicinementioning

confidence: 99%

“…A) The chemical conjugate structure of aptamer‐functionalized TPE TPE‐aptamer and its strategy for IFN‐γ in cells (top); the fluorescence imaging of intracellular IFN‐γ in PBMCs and treated with different concentration of IFN‐γ in the BV2 cells (bottom). Adapted with permission . Copyright 2018, American Chemical Society.…”

Section: Key Application In Biomedicinementioning

confidence: 99%

Biomacromolecule‐Functionalized AIEgens for Advanced Biomedical Studies

Duan

et al. 2019

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The advances in bioinformatics and biomedicine have promoted the development of biomedical imaging and theranostic systems to respectively extend the endogenous biomarker imaging with high contrast and enhance the therapeutic effect with high efficiency. The emergence of biomacromolecule‐functionalized aggregation‐induced emitters (AIEgens), utilizing AIEgens, and biomacromolecules (nucleic acids, peptides, glycans, and lipids), displays specific targeting ability to cancer cell, improved biocompatibility, reduced toxicity, enhanced therapeutic effect, and so forth. This review summarizes the rational design of biomacromolecule‐functionalized AIEgens and their biomedical applications in recent ten years, including high‐resolution optical imaging of cell, tissue, and small animal model with low background; the biomarker detection for early diagnosis and prognosis; the delivery and monitoring of prodrugs; image‐guide photodynamic therapy and its combination with chemotherapy. Through illustrating their functional mechanisms and application, it is hoped that this review would open up a completely new train of research thought for attracted researchers in various fields.

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“…According to the RIM mechanism, any molecules, which can restrict the intramolecular motions of AIEgens by being attached to them, are able to turn on AIE fluorescence . This means that when some AIE probes come into contact with analytes, they may form a confined state to trigger emission.…”

Section: Strategies To Realize Accurate Detection Using Aie Probesmentioning

confidence: 99%

“…As one of the cytokines, interferon‐γ produced mainly by T‐cells and natural killer cells is important for the diagnosis of tuberculosis and other diseases . Ma et al developed 5 (Figure ) consisting of an oligonucleotide that had high specificity toward interferon‐γ to detect trace levels of interferon‐γ secreted by living cells. Target recognition‐dependent RIR drives fluorescence turning‐on.…”

Section: Applications Of Aie Probes In the Field Of Biological Sciencementioning

confidence: 99%

A new approach to developing diagnostics and therapeutics: Aggregation‐induced emission‐based fluorescence turn‐on

Guo

Song

et al. 2019

Medicinal Research Reviews

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Fluorescence imaging is a promising visualization tool and possesses the advantages of in situ response and facile operation; thus, it is widely exploited for bioassays. However, traditional fluorophores suffer from concentration limits because they are always quenched when they aggregate, which impedes applications, especially for trace analysis and real‐time monitoring. Recently, novel molecules with aggregation‐induced emission (AIE) characteristics were developed to solve the problems encountered when using traditional organic dyes, because these new molecules exhibit weak or even no fluorescence when they are in free movement states but emit intensely upon the restriction of intramolecular motions. Inspired by the excellent performances of AIE molecules, a substantial number of AIE‐based probes have been designed, synthesized, and applied to various fields to fulfill diverse detection tasks. According to numerous experiments, AIE probes are more practical than traditional fluorescent probes, especially when used in bioassays. To bridge bioimaging and materials engineering, this review provides a comprehensive understanding of the development of AIE bioprobes. It begins with a summary of mechanisms of the AIE phenomenon. Then, the strategies to realize accurate detection using AIE probes are discussed. In addition, typical examples of AIE‐active materials applied in diagnosis, treatment, and nanocarrier tracking are presented. In addition, some challenges are put forward to inspire more ideas in the promising field of AIE‐active materials.

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“Turn-on” Fluorescent Aptasensor Based on AIEgen Labeling for the Localization of IFN-γ in Live Cells

Cited by 52 publications

References 39 publications

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Fluorogenic Detection and Characterization of Proteins by Aggregation‐Induced Emission Methods

Biomacromolecule‐Functionalized AIEgens for Advanced Biomedical Studies

A new approach to developing diagnostics and therapeutics: Aggregation‐induced emission‐based fluorescence turn‐on

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