Türkiye Yerli Sığır Irklarının Mikrosatellit Belirteçler ile Genetik Karakterizasyonu: Kimliklendirme Çalışmalarında Kullanılabilirliği

Abstract

Objective of this study was to evaluate microsatellite markers in paternity testing of native cattle breeds in Turkey. Blood samples were collected from Anatolian Black (n=51), Anatolian Grey (n=54), South Anatolian Red (n=51), Native Southern Anatolian Yellow (n=51), East Anatolian Red (n=45) and Zavot (n = 19) cattle. From the blood samples DNA was isolated by using a standard phenol/ chloroform method. A total of 20 microsatellite loci were selected from a FAO/ISAG-suggested list. Polymerase chain reaction products were separated by capillary electrophoresis and marker genotypes were determined by fragment analysis. In statistical analyses, allel numbers, observed (Ho) and expected (He) heterozygosities, deviation from Hardy-Weinberg Equilibrium and probability of exclusion (PE) at each microsatellite locus were calculated. A total of 269 different alleles were observed and the mean allele was identified as 13.45. Mean Ho and He values were observed as 0.619-0.852 and 0.669-0.877, respectively. The results indicated that the microsatellite test panel including the most informative 7 loci had total PE value of >0.9999 in each populations and can thereby be used for parentage testing studies of native cattle breeds in Turkey. Keywords

Analysing the genetic diversity and population structure of five native Turkish cattle breeds using SNP data

“…After domestication in Anatolia, cattle spread to South and East Asia and expanded into Europe via the Balkans. Due to its unique bridging position between two continents encompassing various biogeographic regions, Anatolia is a global hub for biological diversity (Arbuckle & Makarewicz, 2009; Çakırlar, 2012; Ozbeyaz et al., 1999; Özşensoy et al., 2014; Özșensoy et al., 2010; Perkins Jr, 1969). Domestic animal genetic resources play a crucial role in agriculture, the economy, science, and culture, both present and future.…”

“…By utilizing SNP data, researchers can effectively analyse the genetic diversity and structure of native Turkish cattle breeds, providing valuable insights for their conservation and breeding programs. In previous studies, Turkish cattle breeds were characterized using low‐resolution markers (Altınalan, 2005; Demir, 2018; Demir & Balcioğlu, 2019; Kuru, 2018; Öner et al., 2019; Özkan, 2005; Özşensoy, 2011; Özşensoy et al., 2014; Özşensoy & Kurar, 2014). Concurrently, population structure and genetic diversity were assessed within and among populations.…”