Türkiye’nin Bingöl ilinde Virüs Belirtisi Gösteren Kavun Bitkilerinde Hıyar mozaik virüsü ve Karpuz mozaik virüsünün Varlığı ve Filogenetik Yakınlıkları

Abstract

In the surveys conducted in the province of Bingöl in 2019, melon plants exhibiting foliar deformations, mosaic pattern in different concentrations of green, and vein banding were noticed. Melon specimens were gathered and screened by RT-PCR using capsid protein (CP) gene-specific primer sets to characterize and to ascertain the possible viral agents related to infected plants. About 657 bp and 822 bp DNA fragments were observed in the agarose gel of infected plants, confirming the presence of Cucumber mosaic cucumovirus (CMV) and Watermelon mosaic potyvirus (WMV). Two related DNA fragments from each recovered from agarose gel randomly were cloned in the proper cloning vector and sequenced by nextgeneration sequencing (NGS). Viral CP sequences obtained were deposited in GenBank (NCBI) with accession number MT361015 and MT361016 for CMV and with accession number MT413451 and 437295 for WMV. Sequences analysis revealed that CMV and WMV isolates showed high sequence consensus with their same species, 99.84%, and 99.88%, respectively. Further analysis disclosed that both Bingöl isolates showed the highest sequence similarity with China isolate (DQ399708). The consensus tree created from various CP sequences in different geographies clearly revealed that the two CMV isolates detected in this study are Subgroup IB, in the very close phylogenetic relations with Turkey, Thailand, India, and China's Subgroup IB isolates from diverse plant origins. Moreover, Bingöl WMV isolates exhibited an evolutionary affinity with isolates from melon and zucchini in France and isolate from a watermelon in China. This work is the first scientific evidence showing infection of CMV and WMV of melon plants in Bingöl province of Turkey, supported by high sequence homology and consensus trees.

Genetic Diversity of Cucumber Mosaic Virus in Cucumber Plants Grown in Diyarbakır Province

“…As a result of RT-PCR performed using primer sets specific to the coat protein (CP) gene, it was determined that the partial coat protein gene length contained 657 bp [29]. Furthermore, two separate research have shown the existence of CMV isolate in the region of Diyarbakır.…”

“…Recently, the research in the world shown that CMV subgroup I divided into two subgroups (Ia and Ib) with the analysis of non-protein coding regions at the 5' end and the CP gene [6]. Previous studies have shown the presence of CMV infections in various hosts in Turkey, as described by several researchers [29,32,36,37,38]. A few studies have shown that subgroup IA isolates are frequently observed in molecular investigations carried with CMV [34,39,40].…”

Serologic and molecular determination and phylogenetic analysis of some viruses infecting pepper in Turkey

“…CMV were clustered three molecular group as IA, IB, and II based on the information about different regions of the genome (Eiras et al, 2004). In previous studies in Turkey, CMV isolates have been determined in different plant hosts (Ergun et al, 2013;Karanfil and Korkmaz 2017;Guneş and Gumuş 2019;Guller and Usta 2020;Yeşil 2020). Most of the Turkish CMV isolates fell into the subgroup IA as the Turkish CMV isolates.…”

“…Most of the Turkish CMV isolates fell into the subgroup IA as the Turkish CMV isolates. Two CMV isolates from cowpea and tobacco crops in Turkey were classified into the subgroup IB (Karanfil and Korkmaz 2017;Guller and Usta 2020). Further sequence data are needed to characterize the population structure of CMV from Turkey and other countries.…”

Güney Marmara Bölgesi Kabakgil Üretim Alanlarında Cucumis melo endornavirus Enfeksiyonunun Belirlenmesi ve İzolatların Moleküler Karakterizasyonu

“…Kabakgilleri enfekte eden virüslerden bazıları yaygın olarak çalışılırken, yeni bulunan bazı virüslerin ise enfeksiyonlarının ne derecede önemli ve yaygın olduğu ise bilinmemektedir. Ülkemizde gerçekleştirilen çalışmalar sonucunda kabakgillerde enfeksiyon meydana getiren çok sayıda virüs hastalığı tanımlanmıştır (Köklü ve Yilmaz, 2006;Kamberoglu ve ark., 2016;Güller ve Usta, 2020;Karanfil, 2022). Bunlar arasından en yaygın olarak tespit edilen virüslerden bazılarının zucchini yellow mosaic virus, watermelon mosaic virus ve cucumber mosaic virus (Topkaya ve ark., 2019;Yeşil, 2021;Karanfil ve Korkmaz, 2021) olduğu bildirilmiştir.…”