Türkiye Kıyılarında Dağılım Gösteren İstilacı Denizkestanesinden (Diadema setosum L., 1778) Kolajen Ekstraksiyonu ve Karakterizasyonu

Abstract

In the present study, collagen was obtained from the tissues of invasive sea urchin (Diadema setosum) rapidly spreading in the Mediterranean. As a result, the yield of collagen isolated from sea urchin was determined to be 23.78±1.33% (dry weight). According to the SDS-Page (Sodium Dodecyl Sulfate-Polyacrylamide gel electrophoresis) analysis, it contains (α1)2α2, (Molecular Weight (MW) 124, 114 kDa) resembling the calfskin collagen and ß chains (MW 245 kDa), similar to the of the sea urchin collagen. In the amino acid analysis of collagen, it was determined that the imino acid (proline+hydroxyproline) content was 196.1 residues/1000 residues. Amide A (3301 cm-1), B (2924 cm-1), I (1643 cm-1), II (1550 cm-1), and III (1242 cm-1) functional bands were visualized in the FTIR spectrum. These results were similar to other collagen sources. For the first was used as a source of collagen in the present study. An alternative collagen source to mammalian collagen, which is used commercially in many industries such as biomedicine, food, and cosmetics, was isolated for the first time from D. setosum, and it was proposed that marine collagen can be used as an alternative collagen source and a functional component in areas including food, cosmetics, and pharmaceutical industries.