Turkish isolates of alfalfa mosaic virus belong to a distinct lineage among global population

Morca, Ali Ferhan; Akbaş, Birol; Santosa, Adyatma Irawan; Topkaya, Şerife; Çelik, Ali

doi:10.1016/j.pmpp.2024.102263

Cited by 1 publication

(1 citation statement)

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“…Studies have shown that plant-based compounds have activity against phytoviruses (Emamzadeh-Yazdi et al, 2018;Song et al, 2021). In other regions of Türkiye, the presence of AMV on various plants has been documented (Morca et al, 2024); nevertheless, information regarding the molecular phylogeny of the pathogen that infects pepper plants in Denizli province, Türkiye, is limited. Therefore, this study is remarkable in terms of contributing to the literature on the existence and spread of AMV in Türkiye.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Coat protein of alfalfa mosaic alfamovirus (AMV) from Türkiye: genetic inference and in silico docking analysis for potential antiphytoviral purposes

DEMİREL,

GÜLLER,

USTA

et al. 2024

Not Bot Horti Agrobo

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In 2021, a study was conducted in the Denizli region of Türkiye to investigate the phylogenetic relationship and presence of alfalfa mosaic alfamovirus (AMV) infecting pepper plants exhibiting viral disease symptoms. A total of 57 samples were collected, of which twenty-four tested positive for AMV with reverse transcriptase polymerase chain reaction (RT-PCR) assays. Samples from pepper plants displaying virus symptoms gave positive bands on the agarose gel, while healthy plants yielded negative results. One of the positive samples was randomly selected, cloned and sequenced. This sequence of the Denizli AMV isolate (753 bp) was recorded in the GenBank database with accession number OQ845956. The nucleotide sequence showed a high nucleotide consensus of 97%-99% compared with the nucleotide sequences of the same variants from different origins in GenBank. According to the phylogenetic tree generated through the Neighbour Joining (NJ) method, this AMV isolate belongs to the same group as Iranian isolates from various of hosts. Furthermore, in silico docking analysis of the coat protein (CP) of the AMV isolate and promising 12 essential oil compounds was performed to enable potential antiviral drug development. Docking study showed that eucalyptol, eugenol and carvacrol can make important contributions to the advancement of drug-based strategies for the managing of plant viruses by interacting with the virus coat protein of high binding energies, -5.3, -5.2 and -5.0 kcal mol-1, respectively. Although the presence of AMV in Denizli province has been reported previously, this study reports the phylogenetic relationships and docking analysis of the new AMV isolate in pepper crops.

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Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%