Turkey rhinotracheitis virus and Escherichia coli experimental infection in chickens: histopathological, immunocytochemical and microbiological study

Majó, Natàlia; Gibert, X.; Vilafranca, M.; O'Loan, C.J.; Allan, Gordon; Costa, Ll.; Pages, Albert; Ramis, Antonio

doi:10.1016/s0378-1135(97)00101-6

Cited by 31 publications

(18 citation statements)

References 19 publications

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“…In both latently or acutely C. psittaci infected turkeys, it was clear that a predisposing C. psittaci infection enhances colonisation and invasion of E. coli in the respiratory tract as well as pharyngeal E. coli excretion. The results of this study are consistent with observations in Bordetella avium-, avian pneumovirus-, Ornithobacterium rhinotracheale-or Mycoplasma meleagridis-infected turkeys receiving an E. coli superinfection [9,14,17,18,20]. However, E. coli excretion titres were not as high as those observed by Van de Zande et al [20] in an APV-E. coli dual infection.…”

Section: Discussionsupporting

confidence: 92%

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Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

et al. 2006

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-Both Chlamydophila psittaci and Escherichia coli infections are highly prevalent in Belgian turkeys and therefore they both might contribute to the respiratory disease complex observed in turkeys. C. psittaci can infect turkeys within the first week of age, even in the presence of maternal antibodies. However, the first C. psittaci outbreaks occur mostly at the age of 3 to 6 weeks, the period when also E. coli infections appear on the farms. Therefore, we examined in this study the pathogenicity of an E. coli superinfection on C. psittaci predisposed turkeys. Turkeys were infected with C. psittaci, E. coli or with C. psittaci followed by E. coli. Simulating the impact of an E. coli infection during the acute phase or the latent phase of a C. psittaci infection, turkeys received E. coli at 1 or 5 weeks post C. psittaci infection, respectively. E. coli superinfection during the acute phase of C. psittaci infection increased C. psittaci excretion and stimulated chlamydial replication in the respiratory tract resulting in exacerbated clinical disease. Interestingly, E. coli superinfection during the latent phase of C. psittaci infection induced chlamydial replication, leading to increased C. psittaci-specific antibody titres. In addition, chlamydial predisposition gave higher E. coli excretion compared with turkeys that had only been infected with E. coli. Overall, the present study clearly demonstrates the pathogenic interplay between C. psittaci and E. coli resulting in more severe respiratory disease. Chlamydophila psittaci / Escherichia coli / turkeys

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Section: Discussionsupporting

confidence: 92%

“…Nevertheless, APEC infections play only a complicating role, requiring predisposing environmental or host factors. Several researchers have demonstrated the exacerbation of respiratory disease in avian pneumovirus-, Bordetella avium-and Mycoplasma meleagridis-infected turkeys by an E. coli superinfection 2 [9,14,17,18,20].…”

Section: Introductionmentioning

confidence: 99%

Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

et al. 2006

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“…The interaction between E. coli and avian metapneumovirus (aMPV) has been studied in more detail. Results obtained are, however, ambiguous (Majo et al, 1997;Parreira & Yano, 1998;Al-Ankari et al, 2001;Vandekerchove et al, 2004).…”

mentioning

confidence: 93%

Multi-locus sequence typing and plasmid profile characterization of avian pathogenicEscherichia coliassociated with increased mortality in free-range layer flocks

et al. 2011

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Avian pathogenic Escherichia coli strains originating from 10 free-range layer flocks were characterized by multi-locus sequence typing and plasmid profile analysis to investigate their phylogenetic relationship and diversity, respectively. In addition to colibacillosis, all flocks tested positive for antibodies against avian metapneumovirus (aMPV) during production, and six of the flocks were concurrently affected by histomonosis. Accumulated average mortality for flocks concurrently affected by colibacillosis and histomonosis made up 17.4%, while the average mortality for E. coli-infected flocks was 16.5%. A total of eight different sequence types (STs) and 47 different plasmid profiles were demonstrated among the E. coli isolates. Within each flock between one and four different STs and between three and 13 different plasmid profiles were demonstrated. A statistical significant difference in STs and plasmid profile diversity of the population of E. coli was not demonstrated between flocks affected by histomonosis compared with histomonosis-free flocks. Only minor clonal diversity was demonstrated for each flock, and in all but one flock colibacillosis started before antibodies against aMPV were detected. All isolates, except two, carried plasmids greater than 100 kb, but only a single plasmid replicon type, IncFIB, was demonstrated, suggesting plasmids representing this type might represent a common pathogenicity factor for the different STs of E. coli. Within each flock a clonal tendency was observed, indicating that only certain clones of E. coli possess a significant pathogenic potential. These clones act as primary rather than secondary pathogens, resulting in colibacillosis without predisposing factors, including histomonosis and aMPV.

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“…Virus genital sistemde özellikle uterus ve ovidukt epitel hücrelerine de yerleşir (15,17). Histopatolojik değişikliklerin görüldüğü 1-14 günlük periyotta, infeksiyon bölgesine Escherichia coli, Bordetella avium, Ornithobacterium rhinotracheale ve Mycoplasma gallisepticum gibi sekonder bakteriyel etkenlerin kolonizasyonu kolaylaşmakta ve sekonder hastalıklara yakalanma olasılığı artmaktadır (2,22,23,26,35).…”

Section: Introductionunclassified

Hindi ve tavuklarda avian pneumovirus infeksiyonlarının indirek floresan antikor testi ve reverse transkriptaz-polimeraz zincir reaksiyonu ile teşhisi

Ica¹

2005

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmada Türkiye'de solunum problemi bulunan tavuk ve hindi sürülerinde Avian pneumovirus (APV) infeksiyonun varlığının belirlenmesi ve sonuçların karşılaştırmalı olarak değerlendirilmesi amaçlandı. Çalışmada 50 tavuk ve 20 hindi sürüsünden toplanan nazal turbinat, nazal svab, akciğer ve trakea örnekleri indirek floresan antikor testi (IFAT) ve reverse transkriptaz-polimeraz zincir reaksiyonu (RT-PCR) teknikleri ile incelendi. RT-PCR ile yapılan çalışmalarda, incelenen tavuk sürüle-rinin %46'sında ve hindi kümeslerinin %50'sinde APV genomu belirlendi. IFAT ile tavuk kümeslerinin %38'inde ve hindi kümesle-rinin %35'inde APV antijenlerinin varlığı saptandı. RT-PCR ile incelenen teşhis materyalleri içinde tavuklarda en yüksek pozitiflik %50 ile nazal turbinatta ve %44.1 ile nazal svabta, hindilerde ise %57.1 ile nazal svabta ve %33.3 ile nazal turbinatta belirlendi. IFAT ile en yüksek pozitiflik aynı materyallerde fakat daha düşük oranlarda tespit edildi. Her iki teknikte de, nazal svab ve nazal turbinatlardan alınan materyallerin akciğer ve trakea örneklerine göre daha uygun olduğu saptandı. APV tanısında RT-PCR'ın IFAT'a göre daha duyarlı olduğu, kullanılan organın ve infeksiyon döneminin sonuçlara etki edebileceği kanısına varıldı.Anahtar sözcükler: Avian pneumovirus, hindi, IFAT, RT-PCR, tavuk. Detection of avian pneumovirus infection in chickens and turkeys by indirect fluorescent antibody test and reverse transcriptase-polymerase chain reactionSummary: The aim of this study was to detect the avian pneumovirus (APV) infection in chicken and turkey flocks with respiratory problems in Turkey. Nasal turbinate, nasal swab, lung and trachea samples collected from 50 chicken and 20 turkey flocks were examined by indirect fluorescent antibody test (IFAT) and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques in the study. APV genome was detected in 46% of chicken flocks and and 50% of turkey flocks by RT-PCR. In IFAT, APV antigens was determined in 38% of chicken flocks and 35% of turkey flocks. On the basis of diagnostic materials used in RT-PCR were considered, the highest positivities were detected in nasal turbinates (50%) and nasal swabs (44.1%) of chickens; and in nazal swabs (57.1%) and nasal turbinates (33.3%) of turkeys. IFAT detected the highest positivity for APV infection in same materials with relatively lower percentages. In both techniques, nasal turbinates and nasal swabs were considered to be superior to the lung and tracheal samples for diagnosis of APV. In conclusion, it was suggested that RT-PCR was more sensitive than IFAT for the detection of APV, but test results might be affected by the test material and the time of infection.

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Turkey rhinotracheitis virus and Escherichia coli experimental infection in chickens: histopathological, immunocytochemical and microbiological study

Cited by 31 publications

References 19 publications

Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

Exacerbation ofChlamydophila psittacipathogenicity in turkeys superinfected byEscherichia coli

Multi-locus sequence typing and plasmid profile characterization of avian pathogenicEscherichia coliassociated with increased mortality in free-range layer flocks

Hindi ve tavuklarda avian pneumovirus infeksiyonlarının indirek floresan antikor testi ve reverse transkriptaz-polimeraz zincir reaksiyonu ile teşhisi

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