Turkevich Method for Gold Nanoparticle Synthesis Revisited

Kimling, Johannes; Maier, Monika; Okenve, B.; Kotaidis, Vassilios; Ballot, H.; Plech, Anton

doi:10.1021/jp061667w

Cited by 1,949 publications

(1,527 citation statements)

References 46 publications

(70 reference statements)

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“…Gold nanoparticles were synthesized following a modification of the Turkevich method. 23 Typically, 0.4 g of HAuCl4.3H2O was dissolved in 90 ml of water in a two-neck round flask (1.3 mM HAuCl4). The resulting solution was heated to boiling and refluxed.…”

Section: Gold Nanoparticle Synthesismentioning

confidence: 99%

DNA delivery to ‘ex vivo’ human liver segments

et al. 2011

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Hydrodynamic injection is an efficient procedure for liver gene therapy in rodents but with limited efficacy in large animals, using an 'in vivo' adapted regional hydrodynamic gene delivery system. We study the ability of this procedure to mediate gene delivery in human liver segments obtained by surgical resection. Watertight liver segments were retrogradely injected from hepatic vein with a saline solution containing a plasmid bearing the enhanced green fluorescent protein (eGFP) gene, under different conditions of flow rate (1, 10 and 20 ml s -1 ) and final perfused volume. Samples were cultured for 1 to 2 days and used for microscopy and molecular analysis of gene expression. The fluorescent and immunohistochemistry studies indicated that in segments injected at X10 ml s -1 , good and wide gene expression was present in the liver sections and the molecular analysis reinforced the histological observation in a quantitative manner (index of apparent gene delivery: 10 2 -10 4 eGFP DNA copy per 100 pg of total DNA; transcription index: 10 5 -2Â10 6 eGFP RNA copy per 100 ng of total RNA). In addition, injected gold nanoparticles (15 nm diameter) suggested that DNA delivery to hepatocytes must involve a facilitated permeation process without membrane disruption. In summary, we show that retrograde venous injection of watertight human liver segment is an anadromous procedure that results in wide liver gene delivery and good gene expression. However, additional studies will be necessary to clarify the influence of the prolonged ischemia injury to hepatocytes in our model.

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Section: Gold Nanoparticle Synthesismentioning

confidence: 99%

DNA delivery to ‘ex vivo’ human liver segments

et al. 2011

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“…Traditionally, the trisodium citrate-based thermal reduction has been broadly recognized as a universal approach for preparing spherical Au nanoparticles (AuNPs) of varied sizes ranging from 15 to 147 nm possessing strong SPR signals [22]. Although this preparation method simply involves controlling the ratios of citrate to Au ions [23], the reaction often needs to be carried out on a relatively large scale at the temperature of boiling water and lacks the reproducible preparation of uniform AuNPs with rather large sizes.…”

Section: Synthesis Of Aunmsmentioning

confidence: 99%

Advanced AuNMs as nanomedicine’s central goals capable of active targeting in both imaging and therapy in biomolecules

Lh¹

2017

Glob Drugs Therap

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“…MUA-capped AuNPs were prepared and concentrated up to 2.4 Â 10 14 NP/mL (0.39 µM) in order to assay their stability in physiologic conditions at significant doses. Note that an AuNP concentration in common synthesis is 0.5-10 nM [27,41]. However, higher concentration of AuNPs is often required for in vivo and in vitro applications.…”

Section: Stability In Physiologic Media Dependence On the Aunps Concmentioning

confidence: 99%

The role of PEG conformation in mixed layers: from protein corona substrate to steric stabilization avoiding protein adsorption

Comenge¹

2015

ScienceOpen Research

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Although nanoparticles (NPs) have been traditionally modified with a single ligand layer, mixture of ligands might help to combine different functionalities and to further engineer the NP surface. A detailed study of the competition between an alkanethiol (11-mercaptoundecanoic acid) and SH-PEG for the surface of AuNPs and the resultant behaviors of this model nanoconjugate is presented here. As a result, the physicochemical properties of these conjugates can be progressively tuned by controlling the composition and especially the conformation of the mixed monolayer. This has implications in the physiological stability. The controlled changes on the SH-PEG conformation rather than its concentration induce a change in the stabilization mechanism from electrostatic repulsion to steric hindrance, which changes the biological fate of NPs. Importantly, the adsorption of proteins on the conjugates can be tailored by tuning the composition and conformation of the mixed layer.

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Turkevich Method for Gold Nanoparticle Synthesis Revisited

Cited by 1,949 publications

References 46 publications

DNA delivery to ‘ex vivo’ human liver segments

DNA delivery to ‘ex vivo’ human liver segments

Advanced AuNMs as nanomedicine’s central goals capable of active targeting in both imaging and therapy in biomolecules

The role of PEG conformation in mixed layers: from protein corona substrate to steric stabilization avoiding protein adsorption

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