Turbiscan Lab® Expert analysis of the stability of ethosomes® and ultradeformable liposomes containing a bilayer fluidizing agent

129

a b s t r a c tIn the present study we have developed lipid nanoparticle (LN) dispersions based on a multiple emulsion technique for encapsulation of hydrophilic drugs or/and proteins by a full factorial design. In order to increase ocular retention time and mucoadhesion by electrostatic attraction, a cationic lipid, namely cetyltrimethylammonium bromide (CTAB), was added in the lipid matrix of the optimal LN dispersion obtained from the factorial design. There are a limited number of studies reporting the ideal concentration of cationic agents in LN for drug delivery. This paper suggests that the choice of the concentration of a cationic agent is critical when formulating a safe and stable LN. CTAB was included in the lipid matrix of LN, testing four different concentrations (0.25%, 0.5%, 0.75%, or 1.0%wt) and how composition affects LN behavior regarding physical and chemical parameters, lipid crystallization and polymorphism, and stability of dispersion during storage. In order to develop a safe and compatible system for ocular delivery, CTAB-LN dispersions were exposed to Human retinoblastoma cell line Y-79. The toxicity testing of the CTAB-LN dispersions was a fundamental tool to find the best CTAB concentration for development of these cationic LN, which was found to be 0.5 wt% of CTAB.

Section: Resultsmentioning

confidence: 93%

“…TurbiscanLab ® is useful to detect destabilization phenomena much earlier and also in a simpler way than other methods, since it is based on the measurement of backscattering (BS) and transmission (T) signals (Araújo et al, 2009;Celia et al, 2009;Marianecci et al, 2010;Liu et al, 2011).…”

Section: Stability Analysis Of Ln By Turbiscanlab ®mentioning

confidence: 99%

Design of cationic lipid nanoparticles for ocular delivery: Development, characterization and cytotoxicity

Fangueiro

Andreani

Egea

et al. 2014

129

“…which assessed the degree of reversible colloidal phenomena such as creaming and sedimentation as well as irreversible phenomena such as coalescence and flocculation (Celia et al, 2009 Measurements were performed at 25ºC and the Turbiscan ® Lab was configured to perform continuous scans, performing a scan every 6 minutes, over a 55mm cell length from bottom to top for an hour. Variation in particle volume fraction (Ф) on particle migration (sedimentation of creaming) and the mean particle diameter (d) due to coalescence resulted in variation in the quantity of transmitted and backscattered light.…”

Section: Determination Of the Stability Of The Optimized Smm Emulsionmentioning

confidence: 99%

“…Variation in particle volume fraction (Ф) on particle migration (sedimentation of creaming) and the mean particle diameter (d) due to coalescence resulted in variation in the quantity of transmitted and backscattered light. The measured amount of transmitted and backscattered light was then interpreted and used to describe the dispersion state (stability) of the emulsion (Mengual et al, 1999;Lemarchand et al, 2003;Celia et al, 2009). …”

Section: Determination Of the Stability Of The Optimized Smm Emulsionmentioning

confidence: 99%

Poly(ethylene glycol) enclatherated pectin-mucin submicron matrices for intravaginal anti-HIV-1 drug delivery

Mashingaidze

Choonara

Kumar

et al. 2016

“…When the sample suffers a creaming process, an increase of backscattering versus time on the top of the vial is observed. If the destabilization phenomenon occurs due to particle aggregation, a backscattering increase versus time can be observed over the whole height of the sample (Celia et al, 2009). If backscattering profiles have a deviation of ≤±2% it can be considered that there are no significant variations on particle size.…”

Section: Optical Stability Assaymentioning

confidence: 99%

Design and physicochemical stability studies of paediatric oral formulations of sildenafil

Provenza¹,

Calpena²,

Mallandrich³

et al. 2014

Personalized medicine is a challenging research area in paediatric treatments. Elaborating new paediatric formulations when no commercial forms are available is a common practice in pharmacy laboratories; among these, oral liquid formulations are the most common. But due to the lack of specialized equipment, frequently studies to assure the efficiency and safety of the final medicine cannot be carried out. Thus the purpose of this work was the development, characterization and stability evaluation of two oral formulations of sildenafil for the treatment of neonatal persistent pulmonary hypertension. After the establishment of a standard operating procedure (SOP) and elaboration, the physicochemical stability parameters appearance, pH, particle size, rheological behaviour and drug content of formulations were evaluated at three different temperatures for 90 days. Equally, prediction of long term stability, as well as, microbiological stability was performed. Formulations resulted in a suspension and a solution slightly coloured exhibiting fruity odour. Formulation I (suspension) exhibited the best physicochemical properties including Newtonian behaviour and uniformity of API content above 90% to assure an exact dosification process.