Turbidimetric measurement of cell biomass of plant cell suspensions

Tanaka, Hideo; Aoyagi, Haruhiko; Jitsufuchi, Tetsuya

doi:10.1016/0922-338x(92)90527-2

Cited by 23 publications

(7 citation statements)

References 13 publications

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“…2). This observation is in agreement with that of Tanaka et al (1992), who reported that there was only a slight change in the size distribution of Catharanthus roseus cell aggregates during the batch culture in a liquid medium. The cell aggregates of Oryza sativa were, however, smaller in the stationary phase than in the logarithmic growth phase.…”

Section: Resultssupporting

confidence: 82%

Gradient of anthocyanin in cell aggregates of Daucus carota in suspension cultures

Madhusudhan

Ravishankar

1996

Biotechnol Lett

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The size distribution of cell aggregates, and the effect of celt aggregate size on anthocyanin content of Daucus carota cells in suspension cultures, was studied. The profile of biomass distribution in various size groups of cell aggregates indicated that over 92% of biomass was present in the aggregates of 500-1500 pm in diameter. The anthocyanin content increased initially with the increase in cell aggregate diameter up to 500-850 pm, and decreased rapidly with the increase in the cell aggregate size above this critical diameter. On the other hand, the surface colour intensity showed a steady increase with the increase in cell aggregate size, indicating a steep radial gradient of anthocyanin content along the radius of the larger cell aggregates.

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Section: Resultssupporting

confidence: 82%

Gradient of anthocyanin in cell aggregates of Daucus carota in suspension cultures

Madhusudhan

Ravishankar

1996

Biotechnol Lett

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“…Optical density measurement has been mainly used for measuring cell concentrations in suspensions of relatively small and unicellular microorganisms [3]. However, it could be also applied to larger and multicellular systems: cyanobacteria [4,5], plant cells [6], and insect cells [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of light intensity on the correlation between cell mass concentration and optical density in high density culture of a filamentous microorganism

Yoon

Shin

Park

et al. 2015

Korean J. Chem. Eng.

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We investigated the effect of light intensity on the correlation between optical density (OD) and cell mass concentration in high cell density culture of Anabaena variabilis. When the light intensity was gradually increased to maintain specific irradiation rate above 10 mmol/s/g dry cell, the dry cell mass concentration to OD ratio changed when the cell density became higher than OD 10, while the correlation was linear when light intensity remained constant after 2.5 days. When the OD was below 10, the dry cell concentration to OD ratio in unicells was the same as that of filaments, indicating that filament length, unicell size, and unicell dry cell concentration did not affect the dry cell concentration to OD ratio. Scanning electron microscope pictures revealed the differences in morphological structures between filaments below and above OD 10. Therefore, we propose that the morphological structures of filaments affected the dry cell concentration to OD ratio.

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“…L. acidophilus NCFM was inoculated into the two simulated systems at cell concentrations of 1 × 10 8 cfu/mL, 1 × 10 9 cfu/mL, and 1 × 10 10 cfu/mL, respectively. Estimation of cell concentrations during cultures was done by turbidimetry [ 20 ]. After incubated at 37°C for 8 hr, the ability of this strain to bind B(a)P was evaluated in terms of its cell concentrations.…”

Section: Methodsmentioning

confidence: 99%

The In Vitro Adsorption Ability of Lactobacillus acidophilus NCFM to Benzo(a)pyrene in PM2.5

Ning

Zhao

et al. 2021

Journal of Toxicology

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The objective of this work was to explore the ability of lactic acid bacteria strains to bind benzo(a)pyrene (B(a)P) existing in PM2.5. In this study, we examined the ability of Lactobacillus acidophilus NCFM to bind B(a)P in the simulated PM2.5 environment. Among the tested 5 strains, Lactobacillus acidophilus NCFM exhibited the best capacity to bind B(a)P, and its B(a)P binding percentage was 60.00%. Simulations of organic and inorganic systems which represent PM2.5 indicated that B(a)P could be absorbed by strain L. acidophilus NCFM. For the inorganic system of pH 5, L. acidophilus NCFM bound 92.74% B(a)P with a cell concentration of 1 × 1010 cfu/mL at 37°C for 8 hr. Regarding the organic system with pH 6, 73.00% B(a)P was bound by strain L. acidophilus NCFM after this bacterium was incubated at 37°C for 10 min. A quick B(a)P binding by this probiotic bacterium took place in the organic system. The removal of B(a)P from PM2.5 was significantly related to incubation time, cultivation temperature, pH, and cell concentration. Thus, our finding shows that long-term consumption of L. acidophilus NCFM is beneficial for the reduction of B(a)P towards the population who are exposed to PM2.5, although the ability of this bacterium to adsorb B(a)P is partly affected by the differences in the origin of PM2.5.

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Turbidimetric measurement of cell biomass of plant cell suspensions

Cited by 23 publications

References 13 publications

Gradient of anthocyanin in cell aggregates of Daucus carota in suspension cultures

Gradient of anthocyanin in cell aggregates of Daucus carota in suspension cultures

Effect of light intensity on the correlation between cell mass concentration and optical density in high density culture of a filamentous microorganism

The In Vitro Adsorption Ability of Lactobacillus acidophilus NCFM to Benzo(a)pyrene in PM2.5

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