Tuning Zr(IV)-assisted peptide hydrolysis at near-neutral pH

Metal assisted peptide bond hydrolysis: Chemistry, biotechnology and toxicological implications

“…In parallel with the reduction of the precipitate amount, the hydrolysis rate for these ligands also decreased [26].…”

“…The most effective ones, presented in Fig. 4, were: 4,13-diaza-18-crown-6; 1,4,10-trioxa-7,13-diazacyclopentadecane; 2-(2-aminoethoxy)-ethanol and HEPES [26].…”

Not just a background: pH buffers do interact with lanthanide ions—a Europium(III) case study

“…Chemical reactions involving complex formation [1,2], oxidation-reduction [3,4], catalytic reactions [5,6] or acid-base reactions [7,8] significantly depend on the solution's hydrogen ion concentration. In case of, e.g., biochemical assays, a small alteration of pH can affect the reaction mechanism and consequently reaction rate, yield, or even the nature of the formed product can vary drastically [9][10][11][12][13]. Therefore, proper maintenance of pH is very important.…”

Hydrolysis of Methionine- and Histidine-Containing Peptides Promoted by Dinuclear Platinum(II) Complexes with Benzodiazines as Bridging Ligands: Influence of Ligand Structure on the Catalytic Ability of Platinum(II) Complexes

“…Typical proteolytic enzymes such as carboxypeptidase A contain a zinc(II) ion in their active sites, thus suggesting that small metal coordination complexes may play the role of a protease [ 2 ]. In recent years, a number of metal complexes including zinc(II) [ 3 , 4 ], cobalt(III) [ 5 ], iron(II/III) [ 6 – 9 ], copper(II) [ 10 – 12 ], nickel(II) [ 13 , 14 ], cerium(IV) [ 15 ], and zirconium(IV) [ 16 , 17 ] have been found to be effective at promoting the hydrolysis of unactivated amide bonds in peptides and proteins. Besides the basic requirement that the complex should be able to promote amide bond cleavage, the selectivity of the cleavage remains a big challenge.…”