“…Details of alkanethiolate SAM/Au substrates preparations are provided in our previous work [12] where monolayers of C 4 -COOH, C 4 -OH, and C 6 -OH were formed on Au substrates by immersing the substrates in 5 mM ethanolic solution of 4-mercaptobutyric acid (HS-C 3 H 6 -COOH, Sigma-Aldrich), 4-mercapto-1-butanol (HS-C 4 H 8 -OH, Sigma-Aldrich), and 6-mercapto-1-hexanol (HS-C 6 H 12 -OH, Sigma-Aldrich), respectively. Thereafter, 150 µl of protein-lipid suspensions were drop casted on the alkanethiolate SAM/Au substrates and incubated for 1 h at 45°C.…”