Tuning surface properties of bone biomaterials to manipulate osteoblastic cell adhesion and the signaling pathways for the enhancement of early osseointegration

Chen, Shoucheng; Guo, Yuanlong; Liu, Runheng; Wu, Shiyu; Fang, Jinghan; Huang, Baoxin; Li, Zhipeng; Chen, Zhuofan; Chen, Zetao

doi:10.1016/j.colsurfb.2018.01.022

Cited by 169 publications

(119 citation statements)

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“…This effect has already been observed in polymers that better expose hydroxyl or carboxylic acid groups at the surface only when hydrated (Ratner, ). Most studies show that a hydrophilic surface is more conductive to the attachment of cells (Chen et al, ). In the present study, a very high statistically significant difference ( p < .001) between the WCA values was obtained when comparing the pure PLA scaffolds group with the composite groups of samples, with mean reduction of a 5.5% (Table ).…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, a very high statistically significant difference ( p < .001) between the WCA values was obtained when comparing the pure PLA scaffolds group with the composite groups of samples, with mean reduction of a 5.5% (Table ). The decrease of the hydrophobicity of the scaffolds surface, coupled with the increase of its roughness and microporosity, led to enhanced metabolic activity of cells adhered to the composite scaffolds (Chen et al, ; Perez & Mestres, ), as shown in Figure . However, the adhesive properties of cells after 7 days of culture appeared to be unaffected by the presence of the additives in the PLA‐based scaffolds (Figure ).…”

Section: Discussionmentioning

confidence: 99%

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Comparison between calcium carbonate and β‐tricalcium phosphate as additives of 3D printed scaffolds with polylactic acid matrix

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Monzón

Wang

et al. 2019

J Tissue Eng Regen Med

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In this study, polylactic acid (PLA)‐based composite scaffolds with calcium carbonate (CaCO3) and beta‐tricalcium phosphate (β‐TCP) were obtained by 3D printing. These structures were evaluated as potential 3D structures for bone tissue regeneration. Morphological, mechanical, and biological tests were carried out in order to compare the effect of each additive (added in a concentration of 5% w/w) and the combination of both (2.5% w/w of each one), on the PLA matrix. The scaffolds manufactured had a mean pore size between 400–425 μm and a porosity value in the range of 50–60%. According to the results, both additives promoted an increase of the porosity, hydrophilicity, and surface roughness of the scaffolds, leading to a significant improvement of the metabolic activity of human osteoblastic osteosarcoma cells. The best results in terms of cell attachment after 7 days were obtained for the samples containing CaCO3 and β‐TCP particles due to the synergistic effect of both additives, which results in an increase in osteoconductivity and in a microporosity that favours cell adhesion. These scaffolds (PLA:CaCO3:β‐TCP 95:2.5:2.5) have suitable properties to be further evaluated for bone tissue engineering applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparison between calcium carbonate and β‐tricalcium phosphate as additives of 3D printed scaffolds with polylactic acid matrix

Donate

Monzón

Wang

et al. 2019

J Tissue Eng Regen Med

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“…However, PLA is a hydrophobic material and, as a consequence, its interaction with the extracellular matrix (ECM) is low [7]. As the adsorption of adhesion-signaling proteins from the ECM is a key step to promote a good osseointegration of the scaffolds [8], the limited affinity of this biomaterial to these substances in the ECM is one of the main drawbacks of PLA-based structures.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

et al. 2020

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3D-printed polylactic acid (PLA) scaffolds have been demonstrated as being a promising tool for the development of tissue-engineered replacements of bone. However, this material lacks a suitable surface chemistry to efficiently interact with extracellular proteins and, consequently, to integrate into the surrounding tissue when implanted in vivo. In this study, aloe vera coatings have been proposed as a strategy to improve the bioaffinity of this type of structures. Aloe vera coatings were applied at three different values of pH (3, 4 and 5), after treating the surface of the PLA scaffolds with oxygen plasma. The surface modification of the material has been assessed through X-ray photoelectron spectroscopy (XPS) analysis and water contact angle measurements. In addition, the evaluation of the enzymatic degradation of the structures showed that the pH of the aloe vera extracts used as coating influences the degradation rate of the PLA-based scaffolds. Finally, the cell metabolic activity of an in vitro culture of human fetal osteoblastic cells on the samples revealed an improvement of this parameter on aloe vera coated samples, especially for those treated at pH 3. Hence, these structures showed potential for being applied for bone tissue regeneration.

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“…[1][2][3][4][5][6][7] Results from these studies have unequivocally established that physicochemical properties such as roughness, topography, surface chemistry, energy and stiffness, direct cell fate by affecting key phenomena including adhesion, proliferation, differentiation, gene and protein expression. [8][9][10][11] Among the factors known to control cellular events, nano-topographical features play a pivotal role by exerting direct cueing on adherent cells. 2,9,10,[12][13][14][15][16][17] Based on this evidence, much effort has been invested in the design of synthetic nanostructured substrates to support the investigation of the interplay between nanotopographical cues and cellular functions.…”

Section: Introductionmentioning

confidence: 99%

<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

Steeves¹,

Ho²,

Munisso³

et al. 2020

IJN

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Introduction: In recent years there has been ample interest in nanoscale modifications of synthetic biomaterials to understand fundamental aspects of cell-surface interactions towards improved biological outcomes. In this study, we aimed at closing in on the effects of nanotubular TiO 2 surfaces with variable nanotopography on the response on human mesenchymal stem cells (hMSCs). Although the influence of TiO 2 nanotubes on the cellular response, and in particular on hMSC activity, has already been addressed in the past, previous studies overlooked critical morphological, structural and physical aspects that go beyond the simple nanotube diameter, such as spatial statistics. Methods: To bridge this gap, we implemented an extensive characterization of nanotubular surfaces generated by anodization of titanium with a focus on spatial structural variables including eccentricity, nearest neighbour distance (NND) and Voronoi entropy, and associated them to the hMSC response. In addition, we assessed the biological potential of a twotiered honeycomb nanoarchitecture, which allowed the detection of combinatory effects that this hierarchical structure has on stem cells with respect to conventional nanotubular designs. We have combined experimental techniques, ranging from Scanning Electron (SEM) and Atomic Force (AFM) microscopy to Raman spectroscopy, with computational simulations to characterize and model nanotubular surfaces. We evaluated the cell response at 6 hrs, 1 and 2 days by fluorescence microscopy, as well as bone mineral deposition by Raman spectroscopy, demonstrating substrate-induced differential biological cueing at both the short-and long-term. Results: Our work demonstrates that the nanotube diameter is not sufficient to comprehensively characterize nanotubular surfaces and equally important parameters, such as eccentricity and wall thickness, ought to be included since they all contribute to the overall spatial disorder which, in turn, dictates the overall bioactive potential. We have also demonstrated that nanotubular surfaces affect the quality of bone mineral deposited by differentiated stem cells. Lastly, we closed in on the integrated effects exerted by the superimposition of two dissimilar nanotubular arrays in the honeycomb architecture. Discussion: This work delineates a novel approach for the characterization of TiO 2 nanotubes which supports the incorporation of critical spatial structural aspects that have been overlooked in previous research. This is a crucial aspect to interpret cellular behaviour on nanotubular substrates. Consequently, we anticipate that this strategy will contribute to the unification of studies focused on the use of such powerful nanostructured surfaces not only for biomedical applications but also in other technology fields, such as catalysis.

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Tuning surface properties of bone biomaterials to manipulate osteoblastic cell adhesion and the signaling pathways for the enhancement of early osseointegration

Cited by 169 publications

References 81 publications

Comparison between calcium carbonate and β‐tricalcium phosphate as additives of 3D printed scaffolds with polylactic acid matrix

Comparison between calcium carbonate and β‐tricalcium phosphate as additives of 3D printed scaffolds with polylactic acid matrix

Evaluation of Aloe Vera Coated Polylactic Acid Scaffolds for Bone Tissue Engineering

<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

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