“…5F,G; two-way ANOVA, Genotype: F(1, 32) = 9.120, p = 0.0049, Time: F(1, 32) = 39.59, p < 0.0001, Interaction: F(1, 32) = 6.651, p = 0.0147; Sidak’s post-hoc test, WT vs KR at End p = 0.0008), PD (Figures 1H,I; two-way ANOVA, Genotype: F(1, 32) = 7.966, p = 0.0081, Time: F(1, 32) = 24.04, p < 0.0001, Interaction: F(1, 32) = 7.966, p = 0.0063; Sidak’s post-hoc test, WT vs KR at End p = 0.0006), and SD (Figures 5L,M; two-way ANOVA, Genotype: F(1, 32) = 32.51, p < 0.0001, Time: F(1, 32) = 36.77, p < 0.0001, Interaction: F(1, 32) = 48.36, p < 0.0001; Sidak’s post-hoc test, WT vs KR at End p < 0.0001) of KR neurons. However, KR SB showed no difference in release compared to WT neurons (Figures 1J,K; two-way ANOVA, Genotype: F(1, 32) = 0.3066, p = 0.5836, Time: F(1, 32) = 8.865, p = 0.0055, Interaction: F(1, 32) = 0.6407, p = 0.4294), which related to our previous findings that Ca 2+ release at secondary branchpoints is reduced (Deng et al, 2021; Reddish et al, 2021).…”