Tuning liver stiffness against tumours: An in vitro study using entrapped cells in tumour-like microcapsules

The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics

“…Contrarily, the technological group was able to duplicate the genetic material and complete the cycle, returning to G0/1 phase with the BrdU label incorporated in the DNA. This indicates that softer matrices present lower resistance to deformation, allowing a significantly faster cell division (Figure 3(C)) (Leal-Egana et al, 2012).…”

“…Moreover, regarding the technological group, the dramatic decrease of proliferation and the failure in the exponential trend by day 45 were probably caused by a decrease in viability ( Figure 1(A,D)). We point out to the enormous aggregates as the most probable factor that originated cell death, possibly due to pore collapse (Leal-Egana et al, 2012), and subsequent limited diffusion of oxygen and nutrients to the inner core.…”

Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model

“…Previous studies show that encapsulation of liver cancer cells into ALG scaffolds may actually enhance cell survival, proliferation [19,20], secretion of ECM proteins [21] and tumor malignancies [22]. Recently, Leal-Egaña et al showed mimic liver fibrosis by using alginate scaffolds could control the proliferation, migration of metastatic HCC cells [23]. Therefore, ALG scaffolds might be more suitable to investigate the pathobiology of HCC cell metastasis.…”

Horizontal Alignment of 5′ -> 3′ Intergene Distance Segment Tropy with Respect to the Gene as the Conserved Basis for DNA Transcription

“…120/80 mmHg) and greater than atmospheric pressure, as true biological system pressure is the force per unit area (kPa) that cells are actually subject to in vivo , as there is pulsatile pressure through inter-endothelial or inter-epithelial junction open cross-sectional surface area in the pressurized biological system in vivo . Support of this supposition comes from two observations of cell macropressurization at extreme ends of the cell macropressurization spectrum:The least, when under atmospheric pressure decreasing underlying substrate stiffness (decreasing stiffness of gel substrate by decreasing cross-linking) [3–5] results in decreased cell proliferation [6] as overall extracellular pressure (atmospheric pressure and substrate pressure) decreases below that of the lowest level of biologically possible macropressurization, which can only be rescued by growth factors [7]; as opposed toThe greatest, when stiff microcapsule-encapsulation [8] or in situ application of neoplastic-level stiff intra-ductal pressure to isolated acini [9] results in intimately apposed-and-juxtaposed cell membrane stiffness, actually increases intracellular pressure [10] and results in cell proliferation [8]. …”

“…The greatest, when stiff microcapsule-encapsulation [8] or in situ application of neoplastic-level stiff intra-ductal pressure to isolated acini [9] results in intimately apposed-and-juxtaposed cell membrane stiffness, actually increases intracellular pressure [10] and results in cell proliferation [8]. …”