Tuning innate immune function using microneedles containing multiple classes of toll-like receptor agonists

Abstract

Microneedle arrays (MNAs) are patches displaying hundreds of micron-scale needles that can penetrate skin. As a result, these arrays efficiently and painlessly access this immune cell-rich niche, motivating significant clinical...

Exploiting Unique Features of Microneedles to Modulate Immunity

“…[ 153 ] Excitingly, differences in APC phenotype were observed by changing the relative ratio of multiple adjuvants. [ 154 ] This highlights another advantage of model antigen delivery with MNAs: the in vivo effect of adjuvant delivery to APCs can be isolated, contributing to rational design approaches for vaccine adjuvants. [ 155,156 ] Knowledge gained from these studies can be applied to other types of disease models.…”

Microneedle array delivery of Yersinia pestis recapitulates bubonic plague

“…MNAs were then washed in distilled water, followed by 70% ethanol. 56 MNAs were imaged on a using a JEOL 6700F FEG-SEM scanning electron microscope to confirm their morphology ( Figure S1 A). 53 To characterize the mechanical properties (stiffness and fracture force) of the MNAs, a Dynamic Mechanical Analyzer (DMA) TA Q800 was used in the static force mode using a compression clamp.…”

Exploiting Unique Features of Microneedles to Modulate Immunity

“…Both antigen and TLRa were effectively delivered to the dermal layers after MNA puncture, leading to accumulation in draining LNs in part due to uptake by targeting the skin-resident APCs 154 . Excitingly, differences in APC phenotype were observed by changing the relative ratio of multiple adjuvants 155 . This highlights another advantage of model antigen delivery with MNAs: the in vivo effect of adjuvant delivery to APCs can be isolated, contributing to rational design approaches for vaccine adjuvants 156,157 .…”