Tuning FRET efficiency as a novel approach for improved detection of naphthalene: application to environmental samples

Abstract

Naphthalene has emission in the ultraviolet (UV) region, limiting its trace level determination in biological and environmental samples due to detrimental effect of UV light on the living cell and interference from other substances having emission in the UV region. Fluorescence resonance energy transfer strategy is adopted for determination of traces naphthalene in the visible region. Significant improvement of lowest detection limit of naphthalene has been achieved through tuning of fluorescence resonance energy transfer efficiency. Anthranilic acid pyrene (ANP) conjugate provides lowest detection limit for naphthalene among three probes studied, viz. ANB, aniline- pyrene conjugate (APA) and ANP. ANP efficiently measures naphthalene content in river water. Copyright © 2016 John Wiley & Sons, Ltd.

Exploring aggregation‐induced emission through tuning of ligand structure for picomolar detection of pyrene

“…The LODs2g, h of L, L1, and L2 for pyrene are 2 × 10 −12 M, 2 × 10 −9 M, and 5 × 10 −9 M while respective association constants are 3.81 × 10 6 M −1 , 7.60 × 10 5 M −1 , and 2.09 × 10 5 M −1 , determined applying the Hill equation (Figure S36–38, ESI). Moreover, the LOD of L for pyrene is 7.8 × 10 −12 M following 3σ/K method2d–f where σ is the standard deviation of the blank, and K is the slope of the calibration curve (Figure S33, ESI, inset plot). Thus, L is effective for detection of pyrene in river and tap water samples .…”

“…Moreover, the LOD of L for pyrene is 7.8 × 10 −12 M following 3σ/K method2d–f where σ is the standard deviation of the blank, and K is the slope of the calibration curve (Figure S33, ESI, inset plot). Thus, L is effective for detection of pyrene in river and tap water samples . The Job's plot indicate 1:1 (mole ratio) interaction between probe and pyrene (Figure S39–41, ESI).…”

“…The developed method has been applied2a–c for pyrene determination in river and tap water samples following standard addition method (Table S6, ESI). To evaluate the accuracy of the method, recovery studies have been performed at different concentration levels.…”

“…Density functional theory (DFT) studies support the experimental facts. The developed method is applied for the determination pyrene in water from Gomti river (India). Solid phase extractive separation of pyrene has been achieved.…”

“…The design and development of appropriate fluorescence probe for selective detection and monitoring of toxic molecules have been a subject of interest in the modern research area . Polycyclic aromatic hydrocarbons (PAHs) are widely distributed and relocated in the environment as a result of incomplete combustion of organic matter . Reactive PAHs belong to 10 most toxic classes of organic compounds (by Centre for Disease Control in 2011) for cell damaging, cytotoxicity, mutagenicity, damage of nervous system, chemical modification of protein, and nucleic acid.…”