Tuning cell cycle of insect cells for enhanced protein production

Wu, Hsuan‐Chen; Hebert, Carla; Hung, Cheng‐Chieh; Quan, David N.; Carter, Karen K.; Bentley, William E.

doi:10.1016/j.jbiotec.2013.08.017

Cited by 6 publications

(2 citation statements)

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“…Expression levels of GFP [44,45], SEAP [46], and the fusion protein Tim4-Fc [47] were 100%, 100%, and 400% higher than the parent cell lines following caspase-1 silencing. Wu et al [48] demonstrated an almost two-fold increase in recombinant GFP expression when silencing of Cyclin E was induced briefly before baculovirus infection.…”

Section: Cell Line Engineeringmentioning

confidence: 99%

Innovations in the Insect Cell Expression System for Industrial Recombinant Vaccine Antigen Production

Cox¹

2021

Vaccines

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The insect cell expression system has previously been proposed as the preferred biosecurity strategy for production of any vaccine, particularly for future influenza pandemic vaccines. The development and regulatory risk for new vaccine candidates is shortened as the platform is already in use for the manufacturing of the FDA-licensed seasonal recombinant influenza vaccine Flublok®. Large-scale production capacity is in place and could be used to produce other antigens as well. However, as demonstrated by the 2019 SARS-CoV-2 pandemic the insect cell expression system has limitations that need to be addressed to ensure that recombinant antigens will indeed play a role in combating future pandemics. The greatest challenge may be the ability to produce an adequate quantity of purified antigen in an accelerated manner. This review summarizes recent innovations in technology areas important for enhancing recombinant-protein production levels and shortening development timelines. Opportunities for increasing product concentrations through vector development, cell line engineering, or bioprocessing and for shortening timelines through standardization of manufacturing processes will be presented.

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Section: Cell Line Engineeringmentioning

confidence: 99%

Innovations in the Insect Cell Expression System for Industrial Recombinant Vaccine Antigen Production

Cox¹

2021

Vaccines

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“…When cyclin E was silenced in Hi5 insect cells, an increase in GFP expression was seen (Wu et al 2013).…”

Section: Improvement Of the Production And Stabilitymentioning

confidence: 99%

Engineering of the baculovirus expression system for optimized protein production

Martínez-Solís

Herrero

Targovnik

2018

Appl Microbiol Biotechnol

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Baculoviruses are arthropod-specific large circular double-stranded DNA viruses successfully used for the control of multiple insect pests. In addition to their application in pest control, baculoviruses have become a versatile and powerful eukaryotic vector for the production of large quantities of recombinant proteins for research and biomedical purposes. Since the first recombinant protein was expressed in 1983 using the baculovirus expression system (BEVS), different strategies have been developed for the generation of recombinant viruses and to increase the stability, yield, and posttranslational modifications of recombinant proteins. In this review, we summarize the main methods and elements playing a role in the BEVS emphasizing recent progresses and future developments with respect to the main aspects involved in protein production using the BEVS.

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Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants

Joon

Bentley

2016

Methods in Molecular Biology

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By use of a strategy incorporating the green fluorescent protein (GFP), facile and rapid monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two techniques for simple determination of virus titer in the baculovirus expression system using GFP co-expression and rapid monitoring of Sf-9 insect cell infection using a combination of GFP and the early-to-late (ETL) promoter of the virus vector. Because of its early appearance, GFP, when placed under the control of ETL promoter, will facilitate vector construction, virus isolation, and titer determination.

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Tuning cell cycle of insect cells for enhanced protein production

Cited by 6 publications

References 50 publications

Innovations in the Insect Cell Expression System for Industrial Recombinant Vaccine Antigen Production

Innovations in the Insect Cell Expression System for Industrial Recombinant Vaccine Antigen Production

Engineering of the baculovirus expression system for optimized protein production

Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants

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