Tuning B cell responses to antigens by cell polarity and membrane trafficking

Batalla, Felipe Del Valle; Lennon-Duménil, Ana-María; Yuseff, María-Isabel

doi:10.1016/j.molimm.2018.06.013

Cited by 9 publications

(6 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed that Vti1b was specifically polarised to the IS upon BCR activation ( Figure 3 ). It has been widely reported that lymphocytes polarise the endocytic/exocytic machinery, as well as other organelles, such as the Golgi apparatus, to the IS ( del Valle Batalla et al, 2018 ; Duchez et al, 2011 ; Ulloa et al, 2022 ; Yuseff et al, 2011 ). Thus, it was not unexpected that Vti1b, which mainly resides in the Golgi and lysosomes, would polarise to the IS.…”

Section: Discussionmentioning

confidence: 99%

The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation

Music

Tejeda-González

Cunha

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

In order to fulfil the special requirements of antigen-specific activation and communication with other immune cells, B lymphocytes require finely regulated endosomal vesicle trafficking. How the endosomal machinery is regulated in B cells remains largely unexplored. In our previous proximity proteomic screen, we identified the SNARE protein Vti1b as one of the strongest candidates getting accumulated to the sites of early BCR activation. In this report, we follow up on this finding and investigate the localisation and function of Vti1b in B cells. We found that GFP-fused Vti1b was concentrated at the Golgi complex, around the MTOC, as well as in the Rab7+ lysosomal vesicles in the cell periphery. Upon BCR activation with soluble antigen, Vti1b showed partial localization to the internalized antigen vesicles, especially in the periphery of the cell. Moreover, upon BCR activation using surface-bound antigen, Vti1b polarised to the immunological synapse, colocalising with the Golgi complex, and with lysosomes at actin foci. To test for a functional role of Vti1b in early B cell activation, we used primary B cells isolated from Vit1b-deficient mouse. However, we found no functional defects in BCR signalling, immunological synapse formation, or processing and presentation of the internalized antigen, suggesting that the loss of Vti1b in B cells could be compensated by its close homologue Vti1a or other SNAREs.

show abstract

Section: Discussionmentioning

confidence: 99%

The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation

Music

Tejeda-González

Cunha

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Efficient uptake and processing of foreign antigens by B cells is critical for their complete activation (1,2). In lymph nodes, B cell activation is initiated by the engagement of the B cell receptor (BCR) with antigens displayed at the surface of neighboring cells, which triggers tyrosine kinase-dependent signaling cascades (3)(4)(5)(6)(7). BCR signaling is coupled to a rapid actin-dependent membrane spreading response, followed by an acto-myosin-dependent contraction phase, enabling B cells to concentrate antigens at the center of the immune synapse (8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction

et al. 2022

Self Cite

View full text Add to dashboard Cite

Upon interaction with immobilized antigens, B cells form an immune synapse where actin remodeling and re-positioning of the microtubule-organizing center (MTOC) together with lysosomes can facilitate antigen extraction. B cells have restricted cytoplasmic space, mainly occupied by a large nucleus, yet the role of nuclear morphology in the formation of the immune synapse has not been addressed. Here we show that upon activation, B cells re-orientate and adapt the size of their nuclear groove facing the immune synapse, where the MTOC sits, and lysosomes accumulate. Silencing the nuclear envelope proteins Nesprin-1 and Sun-1 impairs nuclear reorientation towards the synapse and leads to defects in actin organization. Consequently, B cells are unable to internalize the BCR after antigen activation. Nesprin-1 and Sun-1-silenced B cells also fail to accumulate the tethering factor Exo70 at the center of the synaptic membrane and display defective lysosome positioning, impairing efficient antigen extraction at the immune synapse. Thus, changes in nuclear morphology and positioning emerge as critical regulatory steps to coordinate B cell activation.

show abstract

“…Changes and developments in polarity may be caused by many cellular processes linked to spatial arrangement and protein composition such as cell differentiation, activation of the immune response, and localized membrane growth [64][65][66]. Abnormal polarity behavior may result in impaired function of subcellular organelles as well as the onset and development of various physiological or pathological activities [67].…”

Section: Probes Targeting Polaritymentioning

confidence: 99%

Recent Progress in Fluorescent Probes for Diabetes Visualization and Drug Therapy

Jia

Niu

2022

Chemosensors

View full text Add to dashboard Cite

Diabetes has become one of the most prevalent endocrine and metabolic diseases that threaten human health, and it is accompanied by serious complications. Therefore, it is vital and pressing to develop novel strategies or tools for prewarning and therapy of diabetes and its complications. Fluorescent probes have been widely applied in the detection of diabetes due to the fact of their attractive advantages. In this report, we comprehensively summarize the recent progress and development of fluorescent probes in detecting the changes in the various biomolecules in diabetes and its complications. We also discuss the design of fluorescent probes for monitoring diabetes in detail. We expect this review will provide new ideas for the development of fluorescent probes suitable for the prewarning and therapy of diabetes in future clinical transformation and application.

show abstract

Tuning B cell responses to antigens by cell polarity and membrane trafficking

Cited by 9 publications

References 70 publications

The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation

The SNARE protein Vti1b is recruited to the sites of BCR activation but is redundant for antigen internalisation, processing and presentation

B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction

Recent Progress in Fluorescent Probes for Diabetes Visualization and Drug Therapy

Contact Info

Product

Resources

About