Tungsten disulfide nanosheet and exonuclease III co-assisted amplification strategy for highly sensitive fluorescence polarization detection of DNA glycosylase activity

Zhao, Jingjin; Ma, Yefei; Kong, Rongmei; Zhang, Liangliang; Yang, Wen; Zhao, Shulin

doi:10.1016/j.aca.2015.07.006

Cited by 31 publications

(12 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most sensitive fluorescent assays rely on the formation of a nick after the cleavage by a DNA glycosylase (either bifunctional or coupled with an AP endonuclease), followed by signal amplification. The amplification may be exonuclease-assisted linear isothermal, in which a beacon is annealed and degraded by a double-stranded specific exonuclease in repeated cycles [248][249][250] (Figure 2C). Alternatively, the signal may be enhanced by rolling-circle amplification, using any suitable assay to detect the newly synthesized DNA [251][252][253][254].…”

Section: Assays For Dna Glycosylase Activitymentioning

confidence: 99%

Inhibitors of DNA Glycosylases as Prospective Drugs

Mechetin

Endutkin

Diatlova

et al. 2020

IJMS

View full text Add to dashboard Cite

DNA glycosylases are enzymes that initiate the base excision repair pathway, a major biochemical process that protects the genomes of all living organisms from intrinsically and environmentally inflicted damage. Recently, base excision repair inhibition proved to be a viable strategy for the therapy of tumors that have lost alternative repair pathways, such as BRCA-deficient cancers sensitive to poly(ADP-ribose)polymerase inhibition. However, drugs targeting DNA glycosylases are still in development and so far have not advanced to clinical trials. In this review, we cover the attempts to validate DNA glycosylases as suitable targets for inhibition in the pharmacological treatment of cancer, neurodegenerative diseases, chronic inflammation, bacterial and viral infections. We discuss the glycosylase inhibitors described so far and survey the advances in the assays for DNA glycosylase reactions that may be used to screen pharmacological libraries for new active compounds.

show abstract

Section: Assays For Dna Glycosylase Activitymentioning

confidence: 99%

Inhibitors of DNA Glycosylases as Prospective Drugs

Mechetin

Endutkin

Diatlova

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Besides MoS 2 nanosheets, other TMD nanosheets, such as WS 2 , TiS 2 and TaS 2 , could also be used as effective nanoquenchers in fluorescent sensing . For example, Yuan et al developed a one‐step sonication‐assisted exfoliation method to prepare water‐soluble WS 2 nanosheets as effective fluorescence quenchers for nucleic acid and protein detection (Figure b) .…”

Section: Tmd Nanosheets and Their Composite‐based Optical Sensorsmentioning

confidence: 99%

Recent Advances in Sensing Applications of Two‐Dimensional Transition Metal Dichalcogenide Nanosheets and Their Composites

Ping

Fan

Sindoro

et al. 2017

Adv Funct Materials

233

136

View full text Add to dashboard Cite

of 18) 1605817As a newly emerging class of nanomaterials, 2D TMD nanosheets possess a unique layered structure and large surface areas, as well as outstanding physical, chemical, optical, and electronic properties, which holds great potential for applications in catalysis, [37] sensing, [38] optics, [39] and energy. [40] In this review, we will focus on the introduction of state of the art sensing applications for 2D TMD nanosheets and their composites. In particular, different sensing strategies together with signal-transducing mechanisms in sensors based on 2D TMD nanosheets and their composites are thoroughly discussed. Our aim is to demonstrate a complete concept in this rapidly emerging research area and to address basic knowledge, opportunities, and challenges in this promising field. We expect that more 2D TMD nanosheets and their composites, as well as more sensing strategies, will be explored in this research field, enabling the fabrication of more accurate sensing devices for practical applications. TMD Nanosheets and Their Composite-Based Electrochemical SensorsThe electrochemical method has been extensively recognized as a powerful tool for fast access to biochemical information in complex samples due to its easy operation, fast response, high sensitivity, and low cost. Inspired by the use of graphene as transducers in electrochemical sensors, [41][42][43] researchers have attempted to use 2D TMD nanosheets as a transducer layer. Their good conductivity, large surface area, fast electron transfer kinetics, high signal/noise ratio, and, more importantly, their feasibility for forming composites, make 2D TMD nanosheets attractive as for electrochemical sensors. [44][45][46][47][48][49][50] Table 1 summarizes the recent progress of electrochemical sensors based on 2D TMD nanosheets and their composites. TMD Nanosheets as a Transducer Layer in Electrochemical SensorsIn 2012, our group developed a single-layer TMD-nanosheetbased electrochemical sensor, in which a single-layer MoS 2nanosheet-modified glassy carbon electrode was directly used for selective detection of dopamine in the presence of uric acid Two-dimensional (2D) transition metal dichalcogenide (TMD) nanosheets, such as MoS 2 , WS 2 , etc., are attracting increasing interest due to their intriguing physical, chemical, electronic, and optical properties. Success in development of methods for large-scale production of 2D TMD nanosheets and their composites has given great potential for various novel applications. In this review, recent progress in sensing applications of 2D TMD nanosheets and their composites is introduced. Moreover, different sensing strategies and signal-transducing mechanisms for sensing devices based on 2D TMD nanosheets and their composites are also summarized and discussed.

show abstract

“…The emergence and significant advance of nanotechnology and nanoscience provide new opportunities for the use of nanomaterials in analytical chemistry [32][33][34][35][36][37][38][39]. Recently, a number of nanomaterial, such as carbon nanoparticles (CNPs), gold nanoparticals (AuNPs), metal-organic frameworks (MOFs), SiO2 nanoparticals (SiO2 NPs), graphene oxide (GO), quantum dots (QDs), carbon nanotubes (CNTs), 2D transition metal dichalcogenide nanosheets (TMD NSs), MnO2 nanosheets were introduced into FP/FA sensor system, has brought revolutionary effect for greatly improving detection sensitivity and application range of FP/FA, due to its unique structure and extraordinary properties such as simple/controllable synthesis and modification, different sizes, large volumes and masses, chemical compositions and morphologies, excellent optical and chemical properties [26][27][28][29][30][31][40][41][42][43][44]. Table 1 lists various targets of interest, FP/FA enhancer and signal amplification methods, analytical performance, and so on [40][41][42][43][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60].…”

Section: Nanomaterial-based Fp/famentioning

confidence: 99%