TUNEL assay and SCSA determine different aspects of sperm DNA damage

Henkel, Ralf; Hoogendijk, Christiaan Frederik; Bouic, Patrick; Kruger, T. F.

doi:10.1111/j.1439-0272.2009.01002.x

Cited by 94 publications

(72 citation statements)

References 52 publications

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“…A high correlation between SCSA and TUNEL results ( r = 0.859; p < 0.01) has also been reported by Gorczyca et al [153]. In contrast, Henkel et al could not corroborate the aforesaid findings and concluded that the assays were not comparable [154]. Our own data on TUNEL and SCD to measure sperm DNA fragmentation in a population of men with UI showed that the methods were poorly correlated ( r = 0.28) [127].…”

Section: Clinical Utility Of Sperm Functional Tests In Unexplained Masupporting

confidence: 59%

Role and Significance of Sperm Function in Men with Unexplained Infertility

Esteves¹,

Verza²,

Sharma

et al. 2015

Unexplained Infertility

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Section: Clinical Utility Of Sperm Functional Tests In Unexplained Masupporting

confidence: 59%

Role and Significance of Sperm Function in Men with Unexplained Infertility

Esteves¹,

Verza²,

Sharma

et al. 2015

Unexplained Infertility

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“…A negative correlation between sperm DNA damage and quality of embryo, development of blastocyst, implantation rate has been found in men opting for ICSI when the DNA damage was assessed by ISNT and TUNEL [91][92][93][94].…”

Section: Clinical Aspects and Relevance Of Dna Integrity Testsmentioning

confidence: 99%

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

Shamsi

Imam

Dada

2011

J Assist Reprod Genet

135

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Sperm is not a simple carrier of paternal genetic information but its role extends clearly beyond fertilization. Integrity of sperm genome is an essential pre-requisite for birth of healthy offspring and evaluation of sperm should entail DNA integrity analysis. DNA integrity analysis is a better diagnostic and prognostic marker of sperm reproductive potential. Conventional semen analysis emphasizes on sperm concentration, viability, motility and morphology and has been proven to be a poor indicator of reproductive potential and pregnancy outcome. To overcome the drawbacks associated with conventional semen analysis more useful fertility tests and molecular biomarkers have been explored. Among the different tests which have evolved for assessing the sperm reproductive potential, tests for sperm DNA quality are most promising. Sperm DNA damage has been closely associated with numerous indicators of reproductive health including fertilization, embryo quality, implantation, spontaneous abortion, congenital malformations and childhood diseases. It therefore has great potential as a prognostic test for both in vitro and in vivo conception. This review presents an updated account of tests that have better diagnostic and prognostic implications in the evaluation of sperm DNA damage. The basic principles, outline of methodology, advantage, disadvantage, clinical significance of each technique and implications of these tests have been discussed. The logistics of each test with respect to available resources and equipment in an andrology laboratory, the feasibility of performing these tests in routine diagnostic workup of infertile men and the opportunities and challenges provided by DNA testing in male fertility determination are also presented.

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“…(Alvarez, 2005;Henkel, 2007). The TUNEL and SCSA assays correlate well (Evenson et al, 2007), although they determine different aspects of sperm function (Henkel et al, 2010;Mitchell et al, 2011).…”

Section: The Tunel Assaymentioning

confidence: 99%

The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

Lewis

Aitken

Conner

et al. 2013

Reproductive BioMedicine Online

245

182

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Lewis, S.E.M. et al. (2013). The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment. Astract:Sperm DNA damage is a useful biomarker for male infertility diagnosis and prediction of assisted reproduction outcomes. It is associated with reduced fertilization rates, embryo quality and pregnancy rates, and higher rates of spontaneous miscarriage and childhood diseases. This review provides a synopsis of the most recent studies from each of the authors, all of whom have major track records in the field of sperm DNA damage in the clinical setting. It explores current laboratory tests and the accumulating body of knowledge concerning the relationship between sperm DNA damage and clinical outcomes. The paper proceeds to discuss the strengths, weaknesses and clinical applicability of current sperm DNA tests. Next, the biological significance of DNA damage in the male germ line is considered. Finally, as sperm DNA damage is often the result of oxidative stress in the male reproductive tract, the potential contribution of antioxidant therapy in the clinical management of this condition is discussed. DNA damage in human spermatozoa is an important attribute of semen quality. It should be part of the clinical work up and properly controlled trials addressing the effectiveness of antioxidant therapy should be undertaken as a matter of urgency. IntroductionMale factor infertility is implicated in more than 40% of couples presenting for assisted reproduction treatment. Conventional semen analysis continues to be the only routine test to diagnose this condition even though it is known that such descriptive assessments cannot discriminate between the spermatozoa of fertile and infertile men (Guzick et al., 2001). The shifting values for normality (all 'normal' values now lower) in the fifth edition of the WHO manual (World Health Organization, 2010) compared with the previous WHO editions may result in even less men being classified as infertile (Murray et al., 2012).

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TUNEL assay and SCSA determine different aspects of sperm DNA damage

Cited by 94 publications

References 52 publications

Role and Significance of Sperm Function in Men with Unexplained Infertility

Role and Significance of Sperm Function in Men with Unexplained Infertility

Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility

The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment

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