Tune to immobilize lipases on polymer membranes: Techniques, factors and prospects

Gupta, Shweta; Bhattacharya, Amit; Murthy, C. N.

doi:10.1016/j.bcab.2013.04.006

Cited by 63 publications

(43 citation statements)

References 141 publications

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“…Figure 4(b) showed that white agglomerated granules of Cal-B were adsorbed on the membrane surface. The gravity force allowed the enzyme to fill the vacancies of membrane pores [27,28]. Chauhan et al, Gupta et al, and Wang et al have done lipase immobilized onto several kinds of membranes, and they found that the membrane surfaces had some beads in clusters [24][25][26].…”

Section: Immobilized Lipasementioning

confidence: 99%

The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B)

Widhyahrini

Handayani

Wahyuningrum

et al. 2017

Bull. Chem. React. Eng. Catal.

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Candida antarctica lipase B (Cal-B) has been widely used in the hydrolysis reaction. However, it has some weaknesses, such as: forming of the heavy emulsion during the process, which is difficult to resolve and has no reusability. Therefore, it needs to be immobilized into a suitable matrix. One of the suitable supporting materials is polyethersulfone (PES) and its synthesis becames the objective of this paper. The PES was synthesized via a polycondensation reaction between hydroquinone and 4,4'-dichlorodiphenylsulfonein N-methylpyrrolidone (NMP) as solvent using Microwave Assisted Organic Synthesis (MAOS) method at170 °C for 66 minutes using an irradiation power of 300 watt. The synthesized PES was characterized by FTIR and 1 H-NMR (500 MHz, DMSO-d6). Then the PES membrane was prepared from 20% of the optimized mixtures of PES, PSf (polysulfone), and PEG (polyethylene glycol) dissolved in 80% NMP. The Cal-B was immobilized on the PES membrane by mixing it in a shaker at 30 °C and 100 rpm for 24 h using phosphate buffered saline (PBS). The identification of the immobilized Cal-B was done by using FTIR-ATR spectroscopy and SEM micrographs. The results of Lowry assay showed that the 'Cal-B immobilized' blended-membrane has a loading capacity of 91 mg/cm 2 in a membrane surface area of 17.34 cm 2 . In this work, the activity of immobilized Cal-B was twice higher than the native enzyme in p-NP (p-Nitrophenolpalmitate) hydrolyzing. The results indicated that the synthesized PES showed a good performance when used as a matrix in the immobilization of Cal-B.

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Section: Immobilized Lipasementioning

confidence: 99%

The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B)

Widhyahrini

Handayani

Wahyuningrum

et al. 2017

Bull. Chem. React. Eng. Catal.

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“…Immobilisation of enzymes onto electrospun nanofibres has been examined in a good deal of studies in the last years [37]. The possibility to use a multitude of polymers, organic and inorganic, singly or in combinations (composites or blends) to fabricate electrospun mats and the wide number of procedures available for enzyme immobilisation permit a flexible tailoring of the surface chemistry of nanofibres to generate nanofibrous catalytic mats through several ways.…”

Section: Enzyme Immobilisationmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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“…The yeast Candida rugosa is a well‐established and thoroughly investigated industrial source of seven isoforms of extracellular lipase (CRL) differing in their substrate specificity . CRL is highly applicable in biotransformations of numerous important products such as nutraceuticals, pharmaceuticals, biosensors and aroma esters for the food and cosmetic industries . Substrate or product inhibition together with low operational stability is the main limiting factor for wide application of free lipases.…”

Section: Introductionmentioning

confidence: 99%

Design of biocompatible immobilized Candida rugosa lipase with potential application in food industry

Ivić

Veličković

Dimitrijević

et al. 2016

J. Sci. Food Agric.

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Tune to immobilize lipases on polymer membranes: Techniques, factors and prospects

Cited by 63 publications

References 141 publications

The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B)

The Microwave-assisted Synthesis of Polyethersulfone (PES) as A Matrix in Immobilization of Candida antarctica Lipase B (Cal-B)

Electrospinning-Based Nanobiosensors

Design of biocompatible immobilized Candida rugosa lipase with potential application in food industry

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