Tunable spatiotemporal resolution photoacoustic microscopy by combining quasi-periodic scanning and register-fusion algorithm

Abstract

Quasi-periodic scanning combined with a register-fusion algorithm is proposed to realize tunable spatiotemporal resolution photoacoustic microscopy. Quasi-periodic scanning involves an irrational number ratio for the periods of scanning signals in two directions. It can provide sub-pixel spatial sampling. The proposed method can adjust the temporal and spatial resolutions by changing the data length for image reconstruction. For moving targets, the method can obtain a series of low-resolution images with a high imaging frame rate. A high-spatial-resolution image can be fused from these images using the register-fusion algorithm. The proposed method can acquire both motion and structural details of moving target

Correction of high-rate motion for photoacoustic microscopy by orthogonal cross-correlation

“…Retrospective respiratory gating is used to sort and cluster photoacoustic tomography images by simultaneous capturing of the animal’s respiratory waveform during photoacoustic data acquisition 18 – 24 . Furthermore, quasi-periodic scanning combined with a register-fusion algorithm is introduced to imaging moving targets in PAM 25 . In a word, the efficient method of motion correction is significant to improve the performance of PAM.…”

Temperature difference effect of biological tissues induced by low-intensity unfocused ultrasound

“…ombining the advantages of acoustic and optical imaging, photoacoustic imaging achieves high-resolution imaging by using the contrast of the light absorption coefficient of biological tissue and has received extensive attention as a new medical diagnostic technology. [1][2][3] However, due to the presence of absorbers with extremely high light absorption coefficients like hemoglobin in blood, [4][5][6] the photoacoustic signals of low light absorption coefficient absorbers can be masked. As a result, conventional photoacoustic imaging mainly displays the distribution of components with high light absorption coefficients like blood vessels.…”

Enhancement of all-optical photoacoustic detection sensitivity via elliptical reflector

“…[2][3][4] Currently, most photoacoustic detections still use semi-noncontact or quasi-noncontact methods with ultrasonic transducers as receivers. [5][6][7][8][9] To achieve true contact-free detection, these transducers must be replaced by non-contact detection techniques.…”