Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions

Roberts, G. Seth; Yu, Sam; Zeng, Qinglu; Chan, Leslie C. L.; Anderson, Will; Colby, Aaron H.; Grinstaff, Mark W.; Reid, Steven; Vogel, Robert

doi:10.1016/j.bios.2011.09.040

Cited by 125 publications

(113 citation statements)

References 43 publications

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“…A relatively recent technology to be developed for the characterisation of nanoparticles is based upon tunable resistive pulse sensing (TRPS) [44][45][46][47][48][49][50][51] . TRPS is based on polyurethane elastomeric membranes in which the pore geometry can be altered in real time.…”

Section: Introductionmentioning

confidence: 99%

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

2016

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Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterised as they traverse pores. Previous work using RPS has shown that the size, concentration and zeta potential of the analyte can be measured. Here we use tunable resistive pulse sensing (TRPS) which utilises a tunable pore to monitor the translocation times of nanoparticles with DNA modified surfaces. We start by demonstrating that the translocation times of particles can be used to infer the zeta potential of known standards and then apply the method to measure the change in zeta potential of DNA modified particles. By measuring the translocation times of DNA modified nanoparticles as a function of packing density, length, structure, and hybridisation time, we observe a clear difference in zeta potential using both mean values, and population distributions as a function of the DNA structure. We demonstrate the ability to resolve the signals for ssDNA, dsDNA, small changes in base length for nucleotides between 15-40 bases long and even the discrimination between partial and fully complementary target sequences. Such a method has potential and applications in sensors for the monitoring of nanoparticles in both medical and environmental samples.

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Section: Introductionmentioning

confidence: 99%

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

2016

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“…Technology platforms have also become more robust and reproducible to the extent that assays based upon nanomaterials offer significant advantages over conventional diagnostic systems with regard to assay sensitivity, selectivity, and practicality [6][7][8][9][10][11][12][13] . Following the advancement of material synthesis, a range of new characterisation technologies capable of monitoring and measuring the size, surface chemistry, shape, optical, magnetic and even electrochemical properties have emerged [14][15][16][17][18][19][20] .…”

Section: Introduction -mentioning

confidence: 99%

“…In recent years, nanopore systems utilising Coulter technology have seen resurgence, allowing the characterisation of colloid and nanoparticle based systems 26 . Known collectively as resistive pulse sensing (RPS), they offer an attractive technology format because the measurements provide information on individual particles within their natural environment 14,[27][28][29][30] . RPS has been used to study numerous types of particles in different contexts with impressive resolution, including single molecule biophysics, protein sensing, biological detection 27,28,30 and synthetic nanoparticle characterisation 31,32 .…”

Section: Introduction -mentioning

confidence: 99%

Monitoring Aptamer–Protein Interactions Using Tunable Resistive Pulse Sensing

Billinge

Broom²,

Platt

2013

Anal. Chem.

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-Aptamers are short single-stranded pieces of DNA or RNA capable of binding to analytes with specificity and high affinity. Due to their comparable selectivity, stability and cost, over the last two decades aptamers have started to challenge antibodies in their use on many technology platforms. The binding event often leads to changes in the aptamer's secondary and tertiary structure; monitoring such changes has led to the creation of many new analytical sensors. Here we demonstrate the use of a tunable resistive pulse sensing (TRPS) technology to monitor the interaction between several DNA aptamers and their target -thrombin. We immobilised the aptamers onto the surface of superparamagnetic beads, prior to their incubation with the thrombin protein. The protein binding to the aptamer caused a conformational change resulting in the shielding of the polyanion backbone; this was monitored by a change in the translocation time and pulse frequency of the particles traversing the pore. This signal was sensitive enough to allow the tagless detection of thrombin down to nanomolar levels. We further demonstrate the power of TRPS by performing real time detection and characterisation of the aptamer-target interaction and measuring the association rates of the thrombin protein to the aptamer sequences.

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“…TRPS is an adaptation of RPS in which utilises a conical tunable elastomeric pore that can be stretched or relaxed to change the pore size to suit the sample [11,13,[15][16][17][18][19]. Tunable pore membranes are made of thermoplastic polyurethane and the pores are introduced by mechanical puncture.…”

Section: Tunable Resistive Pulse Sensing (Trps)mentioning

confidence: 99%

“…It was shown that the calculated size of the adenovirus particle was in agreement with the results of TEM and DLS. Subsequently, Roberts and co-workers [19] used tunable pore technology to measure concentrations of Baculovirus. The determined concentrations agreed with those obtained from microscopic counting and flow cytometry.…”

Section: The Role Of Trps In Microbiologymentioning

confidence: 99%

Tunable Resistive Pulse Sensing: Potential Applications in Nanomedicine

Sivakumaran

Platt

2016

Nanomedicine (Lond.)

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An accurate characterisation of nanomaterials used in clinical diagnosis and therapeutics is of paramount importance to realise the full potential of nanotechnology in medicine and to avoid unexpected and potentially harmful toxic effects due to these materials. A number of technical modalities are currently in use to study the physical, chemical and biological properties of nanomaterials but they all have advantages and disadvantages. In this review, we discuss the potential of a relative newcomer, Tunable Resistive Pulse Sensing (TRPS), for the characterisation of nanomaterials and its applications in nanodiagnostics.

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Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions

Cited by 125 publications

References 43 publications

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing

Monitoring Aptamer–Protein Interactions Using Tunable Resistive Pulse Sensing

Tunable Resistive Pulse Sensing: Potential Applications in Nanomedicine

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