Tunable lifetime multiplexing using luminescent nanocrystals

Lu, Yiqing; Zhao, Jiangbo; Zhang, Run; Liu, Yujia; Liu, Deming; Goldys, Ewa M.; Yang, Xusan; Xi, Peng; Sunna, Anwar; Lu, Jie; Shi, Yu; Leif, Robert C.; Huo, Yujing; Shen, Jian; Piper, James A.; Robinson, J. Paul; Jin, Dong-Yan

doi:10.1038/nphoton.2013.322

Cited by 708 publications

(645 citation statements)

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“…We initiated the luminescence kinetics-management study by manipulating the homogenously-doped emitting layer by means of the doping concentration effect, given that upconversion luminescence is highly dependent on dopant concentrations 28,43 . A typical core-shell structure, NaYF 4 :x%Yb 3+ ,y%Er 3+ @NaLuF 4 , was concentration in the isolated pumping core on the temporal behaviors of 543 nm upconversion luminescence (Upper) and the resulting phase angles.…”

Section: Tuning the Emitting Layermentioning

confidence: 99%

“…Inherited from the studies on fluorescent dyes and quantum dots, optical multiplexing strategies using UCNPs have been developed in the spectral domain 7,[24][25][26][27] , time domain 28 and spatial domain 29,30 . However, studies of the complex luminescence mechanisms involving sequential multi-step photophysical and energy transfer processes featuring complex luminescence kinetics are still at their infancy [31][32][33][34] , and may open possibility to develope unique multiplexing strategies in other domains.…”

Section: Introductionmentioning

confidence: 99%

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Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

et al. 2017

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Lanthanide-doped upconversion nanoparticles (UCNPs) are increasingly used as luminescent candidates in multiplexed applications due to their excellent optical properties. Inthepast,several encodingidentities havebeen proposedforUCNPs,includingemissioncolour,intensity ratio between different emissionbands, colourspatial distribution, and luminescencelifetime.In this paper, a new optical encoding dimension for upconversion nanomaterials is developed by exploring their luminescence kinetics, i.e., the phase angle of upconversion luminescence in response to a harmonic-wave excitation. Our theoretical derivation shows that the phase angle is governed jointly by the rise and decay times, characterizing the upconversion luminescence kinetics. Experimentally, a full set of methods are developed to manage the upconversion luminescence kinetics, through which the rise and decay times can be manipulated dependently or independently. Furthermore,a large phase-angle space is achieved in which tens of unique codes can be potentially generated in the same colour channel. Our work greatly extends the multiplexing capacity of UCNPs,and offers newopportunities for their applicationsin a wide range such as microarray assays, bioimaging, anti-counterfeiting, deep tissue multiplexed labelling/detectionand high-density data storage.In addition, the development of thisluminescence kinetics-based optical encoding strategy is also instructive for developing multiplexing techniques using other cascade luminescent systems that inherently lack multi-spectral channels, such as triplet-triplet annihilation molecule pairs.

QC 20170330

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Section: Tuning the Emitting Layermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

et al. 2017

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QC 20170330

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“…(ii) Choosing the most appropriate labelling strategy is also challenging. [114], UCNP [115], or else -for in vitro or in vivo experiment? (v) How could one combine optical microscope with electron microscope, to have a better vision about genome structure?…”

Section: Resultsmentioning

confidence: 99%

Developing bioimaging and quantitative methods to study 3D genome

et al. 2016

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The recent advances in chromosome configuration capture (3C)-based series molecular methods and optical superresolution (SR) techniques offer powerful tools to investigate three dimensional (3D) genomic structure in prokaryotic and eukaryotic cell nucleus. In this review, we focus on the progress during the last decade in this exciting field. Here we at first introduce briefly genome organization at chromosome, domain and sub-domain level, respectively; then we provide a short introduction to various super-resolution microscopy techniques which can be employed to detect genome 3D structure. We also reviewed the progress of quantitative and visualization tools to evaluate and visualize chromatin interactions in 3D genome derived from Hi-C data. We end up with the discussion that imaging methods and 3C-based molecular methods are not mutually exclusive ----actually they are complemental to each other and can be combined together to study 3D genome organization.Keywords: 3D Genome; quantitative methods; bioimaging; super resolution INTRODUCTIONIn eukaryotic nucleus, the organization of chromatin is very complex. Highly condensed chromatin and huge numbers of components, such as transcription factors, chromatin proteins and RNA-processing factors, cram together in a very small volume. Essential nuclear processes such as transcription, replication, and repair occur at spatially defined locations in the nucleus. Accumulating evidence suggests that the expression of many genes is correlated with their positions in cell nucleus, and the spatial organization of chromatins has played essential role in regulating transcriptional activity [1]. How these could be done is one of the greatest challenges in molecular biology. 1 DNA helix is hierarchically packaged into chromatin in an elegant way, and eventually form a chromosome in the eukaryotic nucleus over several levels of higher-order structures [1], indicating that a range of microscopy techniques with spatial and temporal scales that cover several orders of magnitude are necessary to understand the organization principles at different levels in a chromosome. For example, the structure of nucleosomes, the fundamental organization unit of chromatin, has been elucidated by X-ray crystals at nanometer scale [2]. The structure of "beads-on-a-string" fiber, which has a diameter of 11-nm and is the very first level of chromatin organization, is also well-understood through Electron Microscope [33]. However, substructure at the scale of 10-200 nm is poorly understood, especially in live cells, partly due to the requirement of biological system that the dynamics, process and function in biology need to be measured with high spatial (approximately nanometer) and temporal (approximately microsecond to millisecond) † These authors contributed equally to this work.

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“…In general, the concentrations of sensitizers (Yb) and activators (Tm or Er) in upconversion materials primarily determine the luminescence lifetimes 37 . To further assess the doping uniformity during the crystal growth, we collected the luminescence lifetimes of each individual barcoded microrods.…”

Section: Improved Luminescence Quantification By R-osammentioning

confidence: 99%

“…We have also shown recently that luminescence lifetimes of lanthanide-based upconversion materials can be fine-tuned across the microsecond to millisecond range, allowing for creation of temporally multiplexed codes for luminescence detaction 33,37 . In parallel we have developed a controlled synthesis approach for bottom-up production of a library of colour-barcoded heterogeneous micro-rods at low cost 38 .…”

Section: Introductionmentioning

confidence: 96%

High-Precision Pinpointing of Luminescent Targets in Encoder-Assisted Scanning Microscopy Allowing High-Speed Quantitative Analysis

Zheng

Zhao

et al. 2015

Anal. Chem.

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The new agreement specifically addresses what authors can do with different versions of their manuscript -e.g. use in theses and collections, teaching and training, conference presentations, sharing with colleagues, and posting on websites and repositories. The terms under which these uses can occur are clearly identified to prevent misunderstandings that could jeopardize final publication of a manuscript (Section II, Permitted Uses by Authors). Easy Reference User Guide Posting Submitted Works on Websites and Repositories:A digital file of the unedited manuscript version of a Submitted Work may be made publicly available on websites or repositories (e.g. the Author's personal website, preprint servers, university networks or primary employer's institutional websites, third party institutional or subject-based repositories, and conference websites that feature presentations by the Author(s) based on the Submitted Work) under the following conditions:• The posting must be for non-commercial purposes and not violate the ACS' "Ethical Guidelines to Publication of Chemical Research" (see http://pubs.acs.org/ethics).• If the Submitted Work is accepted for publication in an ACS journal, then the following notice should be included at the time of posting, or the posting amended as appropriate: "This document is the unedited Author's version of a Submitted Work that was subsequently accepted for publication in [JournalTitle], copyright © American Chemical Society after peer review. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html]." Note: It is the responsibility of the Author(s) to confirm with the appropriate ACS journal editor that the timing of the posting of the Submitted Work does not conflict with journal prior publication/embargo policies (see http://pubs.acs.org/page/policy/prior/index.html)If any prospective posting of the Submitted Work, whether voluntary or mandated by the Author(s)' funding agency, primary employer, or, in the case of Author(s) employed in academia, university administration, would violate any of the above conditions, the Submitted Work may not be posted. In these cases, Author(s) may either sponsor the immediate public availability of the final Published Work through participation in the feebased ACS AuthorChoice program (for information about this program see http://pubs.acs.org/page/policy/authorchoice/index.html) or, if applicable, seek a waiver from the relevant institutional policy. July, 2016 ABSTRACTCompared with routine microscopy imaging of a few analytes at a time, rapid scanning through the whole sample area of a microscope slide to locate every single target object offers many advantages in terms of simplicity, speed, throughput, and potential for robust quantitative analysis. Existing techniques that accommodate solid-phase samples incorporating individual micron-sized targets generally rely on digital microscopy and image analysis, with in...

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Tunable lifetime multiplexing using luminescent nanocrystals

Cited by 708 publications

References 33 publications

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

Phase angle encoded upconversion luminescent nanocrystals for multiplexing applications

Developing bioimaging and quantitative methods to study 3D genome

High-Precision Pinpointing of Luminescent Targets in Encoder-Assisted Scanning Microscopy Allowing High-Speed Quantitative Analysis

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