Tunable hydrogel composite with two-step processing in combination with innovative hardware upgrade for cell-based three-dimensional bioprinting

Wüst, Silke; Godla, Marie E.; Müller, Ralph; Hofmann, Sandra

doi:10.1016/j.actbio.2013.10.016

Cited by 310 publications

(243 citation statements)

References 25 publications

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“…However, varying HAp concentrations from 0 to 4% did not induce a significant enhancement in the mechanical properties. Incorporation of HAp nanoparticles additionally led to radiopacity and thus visibility of constructed scaffolds under X-ray based medical detection, as shown in Figure 6D [ 16] . Wang et al investigated the effect of bioglass nanoparticles, with the size of 55 nm, on encapsulated SaOS-2 cells.…”

Section: Particle-reinforced Hydrogel Composites 3d Printingmentioning

confidence: 99%

“…In the case of chemical modifications, the introduction of chemical groups and the covalent bonding formations at the interface induce superior interfacial bonding strength of which energy is generally in between the 40 to 400 kJ/ mol i.e. much higher than physical interaction (8)(9)(10)(11)(12)(13)(14)(15)(16) kJ/ mol) [14] . Thus, it is possible to provide substantial increase of mechanical strength to the hydrogel composite system.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with wellunderstood and evaluated conventional manufacturing processes, hydrogel composite 3D printing systems remain a relatively new area of research and much more can be studied with regards to their physicochemical properties such as viscosity, dispersion, reinforcement, and its size and shape [16,17] . In recent years, significant progress has been made in the development of 3D printing systems for hydrogel composites with improved mechanical performance and biofunctionality [1,16,17] . Herein, we first provide a brief introduction of hydrogel composite 3D printing techniques and their application in the field of tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

Jang¹,

Jung²,

Pan³

et al. 2018

ijb

182

117

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Three-dimensional (3D) printing of hydrogels is now an attractive area of research due to its capability to fabricate intricate, complex and highly customizable scaffold structures that can support cell adhesion and promote cell infiltration for tissue engineering. However, pure hydrogels alone lack the necessary mechanical stability and are too easily degraded to be used as printing ink. To overcome this problem, significant progress has been made in the 3D printing of hydrogel composites with improved mechanical performance and biofunctionality. Herein, we provide a brief overview of existing hydrogel composite 3D printing techniques including laser based-3D printing, nozzle based-3D printing, and inkjet printer based-3D printing systems. Based on the type of additives, we will discuss four main hydrogel composite systems in this review: polymer-or hydrogel-hydrogel composites, particle-reinforced hydrogel composites, fiber-reinforced hydrogel composites, and anisotropic filler-reinforced hydrogel composites. Additionally, several emerging potential applications of hydrogel composites in the field of tissue engineering and their accompanying challenges are discussed in parallel.

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Section: Particle-reinforced Hydrogel Composites 3d Printingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

Jang¹,

Jung²,

Pan³

et al. 2018

ijb

182

117

View full text Add to dashboard Cite

show abstract

“…Furthermore, large 3D constructs often require a prohibitive number of cells and also pose the problem of nutrient delivery and cellular waste-product removal. Therefore, we expect that a great deal of further research will be necessary to apply bioprinting to large-scale tissue engineering applications, especially the sizeable 3D tissues that will be necessary for clinically relevant therapies [19,20]. Conversely, notable early success has been observed by culturing 3D printed scaffolds inside of body compartments (e.g., intraperitoneal, intramuscular, subcutaneous and dermal junctional locations).…”

Section: Recent Successes In Btementioning

confidence: 99%

The Potential Impact of Bone Tissue Engineering in the Clinic

et al. 2016

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Bone tissue engineering (BTE) intends to restore structural support for movement and mineral homeostasis, and assist in hematopoiesis and the protective functions of bone in traumatic, degenerative, cancer, or congenital malformation. While much effort has been put into BTE, very little of this research has been translated to the clinic. In this review, we discuss current regenerative medicine and restorative strategies that utilize tissue engineering approaches to address bone defects within a clinical setting. These approaches involve the primary components of tissue engineering: cells, growth factors and biomaterials discussed briefly in light of their clinical relevance. This review also presents upcoming advanced approaches for BTE applications and suggests a probable workpath for translation from the laboratory to the clinic.

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“…The nozzle is easily clogged when the viscosity is too high within the nozzle tip during extrusion [35] ; however, a relatively high viscosity is required to avoid the surface tension-driven droplet formation and the collapse of post-extrusion structure [36] . Thus, a material with shear thinning behaviour and a suitable viscosity will be favoured for 3D printing.…”

Section: Viscosity During Incubation With Mtgasementioning

confidence: 99%

A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl

Zhou¹,

Lee²,

Tan³

2017

ijb

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3D bioprinting is an emerging technology that enables the fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cellcompatibility, printability, structural stability post-printing and bio-functionality to regulate cell behaviours) suitable for printing. Gelatin methacryloyl (GelMA) has recently emerged as an attractive biomaterial in tissue engineering because it satisfies the requirements of bio-functionality and mechanical tunability. However, poor rheological property such as low viscosity at body temperature inhibits its application in 3D bioprinting. In this work, an enzymatic crosslinking method triggered by Ca 2+ -independent microbial transglutaminase (MTGase) was introduced to catalyse isopeptide bonds formation between chains of GelMA, which could improve its rheological behaviours, specifically its viscosity. By combining enzymatic crosslinking and photo crosslinking, it is possible to tune the solution viscosity and quickly stabilize the gelatin macromolecules at the same time. The results showed that the enzymatic crosslinking can increase the solution viscosity. Subsequent photo crosslinking could aid in fast stabilization of the structure and make handling easy.

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Tunable hydrogel composite with two-step processing in combination with innovative hardware upgrade for cell-based three-dimensional bioprinting

Cited by 310 publications

References 25 publications

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

3D printing of hydrogel composite systems: Recent advances in technology for tissue engineering

The Potential Impact of Bone Tissue Engineering in the Clinic

A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl

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