Tunable GUMBOS-based sensor array for label-free detection and discrimination of proteins

Galpothdeniya, Waduge Indika S.; Fronczek, Frank R.; Cong, Mingyan; Bhattarai, Nimisha; Siraj, Noureen; Warner, Isiah M.

doi:10.1039/c5tb02038g

Cited by 33 publications

(14 citation statements)

References 47 publications

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“…W.I.S. Galpothdeniya et al [66] . developed sensor array i. e. TNS‐based GUMBOS (TNS=6‐(p‐toluidino)‐2‐naphthalene sulfonate, fluorescent probe) that were utilized for the label‐free sensitive sensing and segregation of different proteins using LDA (linear discriminant analysis) and PCA (principal component analysis).…”

Section: Synthesis and Applications Of Gumbosmentioning

confidence: 99%

“…W.I.S. Galpothdeniya et al [66] developed sensor array i. e. TNS-based GUMBOS (TNS = 6-(p-toluidino)-2-naphthalene sulfonate, fluorescent probe) that were utilized for the labelfree sensitive sensing and segregation of different proteins using LDA (linear discriminant analysis) and PCA (principal component analysis). The main reason for choosing TNS-based GUMBOS in sensing was the fluorescent nature of TNS in organic solvents and thus its high affinity towards proteins through ionic interactions as well as hydrophobicity.…”

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confidence: 99%

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Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

Kaur

Chopra

2020

ChemistrySelect

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GUMBOS (group of uniform materials based on organic salts) are a new class of solid organic ionic materials that shared similar properties as that of ionic liquids, but have a wider range of melting points from 25°C to 250°C. A large number of combinations have been made to design and synthesize multifunctional GUMBOS with tuned properties that are encouraged to explore these organic materials for various applications. Further, nanomaterials can be fabricated from GUMBOS through various reliable synthetic approaches. These nanomaterials have known as ‚nanoGUMBOS' that possessed advantageous properties at the nanoscale and can be used for target applications. Besides, the size, shape, and uniformity of nanomaterials can be tuned by varying different experimental parameters, and synthetic routes. This review is divided into two parts and presents all the published data regarding GUMBOS and nanoGUMBOS. The first part enclosed the synthesis of GUMBOS as well as their potential applications in the field of analytical, optoelectronics, biology, and sensor technology. In the second part, the synthesis of nanoGUMBOS using different methods and their applications in the field of cellular imaging, optoelectronics, catalysis, enantiorecognition, and sensor technology will be highlighted.

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Section: Synthesis and Applications Of Gumbosmentioning

confidence: 99%

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confidence: 99%

Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

Kaur

Chopra

2020

ChemistrySelect

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“…In this context, it should be mention here that Warner and his coworkers have developed a new type of ILs-based organic salts and coined them as a group of uniform materials based on organic salts (GUMBOS) using the high modularity of ILs. [35][36] According to their suggestion for GUMBOS, the melting point will vary between 25°C to 250°C. Hence, the organic salts with a melting point between 25°C and 100°C are ILs as well as GUMBOS.…”

Section: Introductionmentioning

confidence: 99%

“…Due to their significant properties and fine tunablity, GUMBOS have become potential candidates in versatile fields like extraction, separation, sensors, biocatalysis, and electrochemistry, fabrication of active pharmaceutical ingredient loaded drug carriers, microemulsion, and energy conversion field in the last decades. [27,[36][37][38][39][40][41][42][43][44][45][46] Since the acid-base equilibrium process occurs in aqueous solutions and non-polar to polar organic solvents, several traditional organic dyes having capability as a chromogenic indicator in aqueous solutions have been modified so far within the building blocks of ILs for the visualization of acid-base equilibrium process in non-aqueous solvents. [45][46][47] Potentially, these IL-integrated acid-base sensors may have versatile applications that avoid using volatile solvents and allow strong interactions with analytes because of their high concentrations, thus increasing practical applications.…”

Section: Introductionmentioning

confidence: 99%

Ionic Liquids based Acid‐base Indicators for Aqueous to the Non‐Aqueous Medium: An Overview

Rahman

Das

2021

ChemistrySelect

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Visualizing acid-base equilibria and determining acid-base equilibrium constants (pK a ) in aqueous to the non-aqueous medium is essential for understanding the various chemical and biochemical processes. This mini review highlights the possibility of fine-tuning the traditional acid-base indicator dye's physicochemical properties into ionic liquids (ILs)-integrated ones to enhance their solubility and practical applications in a wide range of solvents. These include the high sensitivity towards acid and bases by virtue of its colour and spectral properties changes. ILs-based indicators could be used to determine the pK a values in aqueous to the non-aqueous medium from polar to non-polar ones by overlapping indicator method. This review provides an essential idea of the emerging challenges and openings that may motivate the scientific communities to move towards IL-integrated acid-base indicators and find their dissociation constants (pK Ind ) in any solvents depending on their solubility. The calculated pK Ind values could be used to determine the pK a values of different acids and bases in any solvents. It would also be beneficial to find a general trend of acidity and basicity from solvents to solvents using such types of indicators without alternating the main moiety. It will benefit the scientific communities to understand the pK a scales in different solvents.

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“…A subsequent pattern-recognition of the thus-obtained signatures enables the accurate identification of proteins. Signature-based sensing has been successfully employed for the detection of proteins in dilute solutions [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ] and in biological matrices [ 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. However, most of the current examples require the measurement of optical signals from spatially-separated materials for the generation of signatures, e.g., in multiple wells of a microplate, which significantly limits the scope for applications that depend on a simple and rapid identification of proteins.…”

Section: Introductionmentioning

confidence: 99%

A Multi-Fluorescent DNA/Graphene Oxide Conjugate Sensor for Signature-Based Protein Discrimination

Tomita

Ishihara

Kurita

2017

Sensors

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Signature-based protein sensing has recently emerged as a promising prospective alternative to conventional lock-and-key methods. However, most of the current examples require the measurement of optical signals from spatially-separated materials for the generation of signatures. Herein, we present a new approach for the construction of multi-fluorescent sensing systems with high accessibility and tunability, which allows generating protein fluorescent signatures from a single microplate well. This approach is based on conjugates between nano-graphene oxide (nGO) and three single-stranded DNAs (ssDNAs) that exhibit different sequences and fluorophores. Initially, the three fluorophore-modified ssDNAs were quenched simultaneously by binding to nGO. Subsequent addition of analyte proteins caused a partial recovery in fluorescent intensity of the individual ssDNAs. Based on this scheme, we have succeeded in acquiring fluorescence signatures unique to (i) ten proteins that differ with respect to pI and molecular weight and (ii) biochemical marker proteins in the presence of interferent human serum. Pattern-recognition methods demonstrated high levels of discrimination for this system. The high discriminatory power and simple format of this sensor system should enable an easy and fast evaluation of proteins and protein mixtures.

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Tunable GUMBOS-based sensor array for label-free detection and discrimination of proteins

Abstract: We report a sensor array approach, based on a novel group of 6-(p-toluidino)-2-naphthalenesulfonate (TNS)-based organic salts, for sensitive and label-free sensing of proteins.

Cited by 33 publications

References 47 publications

Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

Ionic Liquids based Acid‐base Indicators for Aqueous to the Non‐Aqueous Medium: An Overview

A Multi-Fluorescent DNA/Graphene Oxide Conjugate Sensor for Signature-Based Protein Discrimination

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