Tunable diode laser absorption spectroscopy as method of choice for non-invasive and automated detection of microbial growth in media fills

Brueckner, David; Roesti, David; Zuber, Ulrich; Sacher, Meik; Duncan, D. W.; Krähenbühl, Stephan; Braissant, Olivier

doi:10.1016/j.talanta.2017.01.088

Cited by 11 publications

(6 citation statements)

References 5 publications

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“…In addition, such instruments are under a constant flow of nitrogen and therefore require a fair amount of that gas. TDLAS has been used in microbiology recently for the detection of bacterial growth in pharmacological samples and clinical samples (such as blood cultures) based on O 2 and CO 2 concentrations in vials (Brueckner et al, 2016(Brueckner et al, , 2017Duncan et al, 2016;Shao et al, 2016Shao et al, , 2018). Furthermore, it is possible to detect bacterial growth linked to cystic fibrosis or the presence of Helicobacter pylori infection directly from exhaled air (Henderson et al, 2018)…”

Section: Oxygenmentioning

confidence: 99%

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

et al. 2020

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Viability and metabolic assays are commonly used as proxies to assess the overall metabolism of microorganisms. The variety of these assays combined with little information provided by some assay kits or online protocols often leads to mistakes or poor interpretation of the results. In addition, the use of some of these assays is restricted to simple systems (mostly pure cultures), and care must be taken in their application to environmental samples. In this review, the necessary data are compiled to understand the reactions or measurements performed in many of the assays commonly used in various aspects of microbiology. Also, their relationships to each other, as metabolism links many of these assays, resulting in correlations between measured values and parameters, are discussed. Finally, the limitations of these assays are discussed.

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Section: Oxygenmentioning

confidence: 99%

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

et al. 2020

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“…Mature M. smegmatis and M. tuberculosis biofilms were further tested for oxygen and carbon dioxide concentration changes in sealed vial headspaces using a TDLAS spectrometer (Lighthouse Instruments, Charlottesville, VA, USA). Measurements were performed as previously described 29 , 30 . The sample set used for this measurement was prepared similarly to those used for the calorimetric measurements.…”

Section: Methodsmentioning

confidence: 99%

“…Using this approach avoids the presence of planktonic cells, as very few will exist on such a membrane, enabling the study of a mature undisturbed biofilm. In addition to IMC, Tunable Diode Laser Absorption Spectroscopy (TDLAS) also allows non-invasive measurement of oxygen and carbon dioxide concentrations in the headspace to determine biofilm respiration rate 28 , 29 . Most mycobacteria are considered strictly aerobic, consuming oxygen and releasing carbon dioxide (Equation 1 ), so TDLAS is therefore well suited to gather metabolic information.…”

Section: Introductionmentioning

confidence: 99%

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

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Bruckner

Bonkat³

et al. 2017

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Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of mycobacterial biofilms with two independent non-invasive techniques (isothermal microcalorimetry and tunable diode laser absorption spectrometry), mature biofilms of slow- and fast-growing species appeared more alike than expected. Metabolic heat production rate was 2298 ± 181 µW for M. smegmatis and 792 ± 81 µW for M. phlei, while M. tuberculosis and M. bovis metabolic heat production rates were between these values. These small differences were further confirmed by similar oxygen consumption rates (3.3 ± 0.2 nMole/s and 1.7 ± 0.3 nMole/s for M. smegmatis and M. tuberculosis, respectively). These data suggest that the metabolic potential of slow-growing mycobacterial biofilms has been underestimated, particularly for pathogenic species.

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“…(site isolate), Bacillus subtilis (ATCC 6633), Aspergillus brasiliensis (ATCC 16404) and Pseudomonas aeruginosa (ATCC 9027) and were incubated in the Tam48 at 25 AE 0Á1°C in TSB. Clostridium sporogenes (ATCC 19404) was selected as model organism for anaerobe studies and cultured in FTM at 32Á5 AE 0Á1°C in the Tam48 calorimeter (for details about creation of microbial suspensions see (Brueckner et al 2017)). The rationale for selecting the micro-organisms intended to include one house isolate with high growth variability (and long lag phase) and to cover the pharmacopoeia strains in use excluding fast growing species.…”

Section: Methodsmentioning

confidence: 99%

An alternative sterility assessment for parenteral drug products using isothermal microcalorimetry

et al. 2017

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Tunable diode laser absorption spectroscopy as method of choice for non-invasive and automated detection of microbial growth in media fills

Cited by 11 publications

References 5 publications

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

A Review of Methods to Determine Viability, Vitality, and Metabolic Rates in Microbiology

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

An alternative sterility assessment for parenteral drug products using isothermal microcalorimetry

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