Tuna blood inhibits lipopolysaccharide-induced inflammatory mediators in RAW264.7 macrophages

Abstract

Background: This study aimed to evaluate the anti-inflammation effects of the freeze-dried tuna whole blood (FTB), and freeze-dried tuna blood cell (FTC) in LPS-induced RAW264.7 cells.Methods: The RAW264.7 cells were pre-administered with FTB and FTC at different concentrations for 2 h and then stimulated with lipopolysaccharide (LPS) for 24 h. The production of reactive oxygen species (ROS), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) in RAW 264.7 cells were then determined.Results: The results showed that only FTB remarkably abolished LPS-induced ROS in RAW264.7 cells. FTB and FTC significantly decreased LPS-induced NO which IC50 values of FTB and FTC after 24 h were 78.58 and 22.47 μg/mL, respectively. TNF-α and IL-1β secretion were abolished by FTB and FTC in LPS-stimulated macrophages which IC50 values of both FTB and FTC after 24 h were more than 25 μg/mL, respectively. However, the efficacy of FTC against inflammatory mediators was due to cytotoxic effects on RAW 264.7 cells.Conclusion: Tuna whole blood potentially inhibits inflammation through modulating the synthesis of several mediators and cytokines associated with the development of inflammation. These findings suggest a role of tuna blood on anti-inflammatory activity.Keywords: Anti-inflammatory activity, RAW 264.7 cells, red blood cell, tuna blood, waste utilization

Physical, chemical composition and umami compound of dried immature and mature roes of skipjack tuna (Katsuwonus pelamis)

“…Skipjack tuna (Katsuwonus pelamis) is the primary commercial tuna species for canned tuna production, accounting for more than 70% of total imported tuna (Department of Fish-eries, 2022). Large quantities of by-products (50%-55% of total weight) are generated from tuna processing and include head, skin, bone, viscera, washing water, blood and roe (Klaypradit et al, 2021), so maximal utilization of these materials will reduce waste and also promote economic sustainability.…”