Tumours of the Nervous System in Mice treated Neonatally with N-Ethyl-N-nitrosourea

Searle, C. E.; Jones, E. L.

doi:10.1038/240559a0

Cited by 26 publications

(8 citation statements)

References 8 publications

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“…The incidences of some of these tumours among survivors at 40 weeks of age are reported in Table IV. A significant increase (P < 0.01) of the proportion of mice dying with lung adenomata was found in both sexes in (Graffi and Hoffmann, 1966;Terracini and Stramignoni, 1967;Kelly et al, 1968;Frei, 1970;Joshi and Frei, 1970;Terracini and Testa, 1970;Frei, 1971). The sporadic occurrence of neurogenic tumours in the present series as well as in previous studies differs from findings following a single administration of the related compound N-nitroso-N-ethylurea (Searle and Jones, 1972;Vesselinovitch et al, 1974).…”

Section: Other Tumourscontrasting

confidence: 55%

The roles of age at treatment and dose in carcinogenesis in C3Hf/Dp mice with a single administration of N-nitroso-N-methylurea

Terracini¹,

Testa²,

Cabral³

et al. 1976

Br J Cancer

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Summary.-C3Hf/Dp;-mice were given a single i.p. injection of 50, 25 or 5 ,ug/g N-Nitroso-N-Methylurea (NMU) at either I or 70 days of age or 50 flg/g at 21 days of age. They were olbserved until death or until 120 weeks of age.The two highest doses of NMU produced tumours in a wide spectrum of organs, including the thymus, forestomach, lung, liver (only in males), kidneys, ovaries and orbital glands.The only two tumo'lr types which appeared to be closely related to the occurrence of death were thymic lymphomata (most of which were-found in mice dying before 40 weeks after treatment) and carcinomata of the forestomach. Lifetime analyses are presented concerning the occurrence of these two tumour types as well as the occurrence of any tumour'after 40 weeks of age or since treatment.Incidences of thymic lymphomata were 67^60%, 39-0°and 21-20% in mice receiving 50 ,ug/g NMU at 1, 21 and 70 days respectively and 17.1% in mice receiving 25 ug/g at 1 day. In'the other groups the' incidence of thymic lymphomata was zero or negligible. The rate of progression of thymic lymphomata until death was related to both earliness of treatment and dose. On the contrary, incidences and progression of carcinomata of the forestomach were unrelated to age at treatment. Since breakdown of NMU is very rapid and does not require enzymes, these results are considered as evidence that host-tumour interaction differs from organ to organ.No excess of tumours over the controls was found in mice receiving 5 ,ug/g either at 1 or 70 days of age.

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Section: Other Tumourscontrasting

confidence: 55%

The roles of age at treatment and dose in carcinogenesis in C3Hf/Dp mice with a single administration of N-nitroso-N-methylurea

Terracini¹,

Testa²,

Cabral³

et al. 1976

Br J Cancer

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“…Some of these tumours were recorded in a preliminary report (Searle and Jones, 1972) a more detailed account of their neuropathological features is in press (Jones et al, 1976). Liver tumours These were found in 65% of C57BL mice examined post mortem, figures for other strains being: A, 11%; DBAf, 28%; IF, 7%.…”

Section: Nervous System Tumoursmentioning

confidence: 99%

“…We were most interested to find a small number of neural tumours in mice of the DBAf and IF strains, and a brief account of the first 10 mice with such tumours has been published (Searle and Jones, 1972). By the end of the experiment the total had risen to 14, including one C57BL mouse.…”

mentioning

confidence: 99%

The multipotential carcinogenic action of N-ethyl-N-nitrosourea administered neonatally to mice

Searle¹,

Jones²

1976

Br J Cancer

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Summary.-Newborn A, C57BL, DBAf and IF mice were injected s.c. with a range of doses of N-ethyl-N-nitrosourea (ENU). A high proportion of treated mice developed tumours, particularly hepatomata, pulmonary adenomata and carcinomata, and malignant lymphomata of thymus and spleen. Liver tumours occurred most frequently in C57BL and DBAf mice, lung tumours in A mice, and lymphomata in A and DBAf mice. A small proportion of C57BL, DBAf and IF mice developed tumours of the nervous system. The results are discussed with reference to the ready induction of nervous system tumours in similarly treated rats, and their relevance for human cancer.

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“…7; Fields, unpublished) to induce more nervous system tumors in mice, so that only a few are available. In contrast, neural tumors can be induced in the rat with remarkably high efficiency and specificity by use of a carcinogen, ethylnitrosourea (8).…”

mentioning

confidence: 99%

New cell surface antigens in rat defined by tumors of the nervous system.

Fields¹,

Gosling²,

Megson³

et al. 1975

Proc. Natl. Acad. Sci. U.S.A.

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Tumors of the central and peripheral nervous system were induced in rats with ethylnitrosourea. Many of these tumors were transplanted in syngeneic recipients, and several cell lines were derived from them.An antiserukn raised against one such cell line in C3H mice defined two cell surface antigens in cytotoxicity tests. One, the common antigen, was present on rat brain and embryonic tissues and was present in large amounts on most tumors or cell lines from the nervous system. Fibroblastic cell lines had smaller amounts of this antigen, which also could be detected by immunofluorescence. The other, restricted antigen was not detected on normal or embryonic tissues. It was present on six tumors from the nervous system, on one glial cell line, and on a Schwanncell line RN22. In addition, it was present on four out of eleven cloned cell lines isolated from rat tumors at the Salk Institute. Two of the positive clonal lines had been shown to have properties unique to neuronal cells. The restricted antigen was therefore expressed on the cell surface of some, but not all, glial, Schwann, and neuronal neoplastic cells.The study of neural* cells in tissue culture provides a new approach to questions of the functions and interactions of the different populations of cells in the nervous system. However, physical methods for separating the different cell types have severe limitations, and identification of cells that have been treated with enzymes or grown in tissue culture is time-consuming and difficult. The use of surface antigens for distinguishing, separating, and studying the function of different types of lymphocytes (1) has suggested a possible immunological approach to the problems of neural cell populations.A number of murine allo-antigens (Thy-i, PC1, Sk, are known to be present in mouse brain; if any one of them were restricted to a subset of neural cells, it might be a useful marker (2). In addition, new cell surface markers have been defined by using tumors of the nervous system. Recently an antigen on brain and murine neuroblastoma C1300 (3), and a glial-specific brain antigen carried by oligodendroglioma G26 have been reported. The restricted distribution of the glialspecific antigen was determined by using five more tumors of nervous system, and several leukemias and sarcomas, but its distribution on the cell types in normal brain has not yet been determined directly (4). A third cell surface antigen has been defined by taking advantage of the clones of neuroblastoma C1300 and their capacity for morphological differentiation in tissue culture, to reveal an antigen shared by brain and only the differentiated cells in vitro (5, 6). Again, the antigenbearing cell type in normal brain, and the possible restriction of the antigen to neurons, remain open questions.While the murine tumors are an attractive system, it has proved difficult (ref. 7; Fields, unpublished) to induce more nervous system tumors in mice, so that only a few are available. In contrast, neural tumors can be induced in the rat with rem...

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Tumours of the Nervous System in Mice treated Neonatally with N-Ethyl-N-nitrosourea

Cited by 26 publications

References 8 publications

The roles of age at treatment and dose in carcinogenesis in C3Hf/Dp mice with a single administration of N-nitroso-N-methylurea

The roles of age at treatment and dose in carcinogenesis in C3Hf/Dp mice with a single administration of N-nitroso-N-methylurea

The multipotential carcinogenic action of N-ethyl-N-nitrosourea administered neonatally to mice

New cell surface antigens in rat defined by tumors of the nervous system.

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