Tumour-suppressing functions of the lncRNA MBNL1-AS1/miR-889-3p/KLF9 axis in human breast cancer cells

Jin, Yongmei; Xu, Lei; Zhao, Bin; Bao, Wenqing; Ye, Ying; Yang, Tiehong; Sun, Qiyu; Liu, Jianping

doi:10.1080/15384101.2022.2034254

Cited by 9 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MBNL1-AS1 is a novel lncRNA discovered in recent years. Previous studies have proved that MBNL1-AS1 plays a tumor-suppressive role in several types of cancers, such as breast cancer, lung cancer, and bladder cancer [34][35][36]. Te role of MBNL1-AS1 in PAAD has not been clarifed until our study.…”

Section: Discussionmentioning

confidence: 73%

LncRNA MBNL1-AS1 Suppresses Cell Proliferation and Metastasis of Pancreatic Adenocarcinoma through Targeting Carcinogenic miR-301b-3p

et al. 2023

View full text Add to dashboard Cite

Pancreatic adenocarcinoma (PAAD) has been a huge challenge to public health due to its increasing incidence, frequent early metastasis, and poor outcome. The molecular basis of tumorigenesis and metastasis in PAAD is largely unclear. Here, we identified a novel tumor-suppressor long noncoding RNA (lncRNA) MBNL1-AS1, in PAAD and revealed its downstream mechanism. Quantitative real-time PCR (qRT-PCR) data showed that MBNL1-AS1 expression was significantly downregulated in PAAD tissues and cells, which was closely associated with metastasis and poor prognosis. Cell counting kit-8 (CCK-8) assay, transwell assay, and western blot verified that overexpression of MBNL1-AS1 suppressed cell proliferation, migration, and epithelial mesenchymal transformation (EMT) behavior in PAAD cells. By using a dual luciferase reporter gene system, we confirmed that miR-301b-3p was a direct target of MBNL1-AS1. Further mechanismic study revealed that upregulation of miR-301b-3p abolished the inhibitory effect of MBNL1-AS1 overexpression on cell proliferation, tumorigenesis, migration and EMT. Our results demonstrate that MBNL1-AS1 plays a tumor-suppressive role in PAAD mainly by downregulating miR-301b-3p, providing a novel therapeutic target for PAAD.

show abstract

Section: Discussionmentioning

confidence: 73%

LncRNA MBNL1-AS1 Suppresses Cell Proliferation and Metastasis of Pancreatic Adenocarcinoma through Targeting Carcinogenic miR-301b-3p

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Moreover, KLF9 is reportedly under-expressed in OS cells and tissues and it participates in modulating the malignant progression of OS [ 55 , 56 ]. Reportedly, miR-889 targets KLF9 in non-small cell lung cancer and breast cancer cells [ 17 , 18 ]. Similarly, this study proved that miR-889 could target KLF9 in OS cells.…”

Section: Discussionmentioning

confidence: 99%

“…KLF9 can inhibit the progression of OS [ 16 ]. In addition, the miR-889/KLF9 axis has been found to play a key role in the pathogenesis of non-small cell lung cancer and breast cancer [ 17 , 18 ]. However, it is unclear whether the miR-889/KLF9 axis is involved in OS progression.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA_0078767 upregulates Kruppel-like factor 9 expression by targeting microRNA-889, thereby inhibiting the progression of osteosarcoma

2022

View full text Add to dashboard Cite

Among kids and juveniles, osteosarcoma (OS) is a common bone malignancy. Circular RNAs (circs, circRNAs) play important roles in multiple malignancies including OS, yet circ_0078767ʹs biological functions in OS are far from well elucidated. This study is targeted at understanding circ_0078767ʹs biological functions in OS and its molecular mechanisms. This study confirmed that circ_0078767 expression was reduced in OS cell lines and tissues. Circ_0078767 overexpression remarkably inhibited OS cell growth, migration, invasion, epithelial-mesenchymal transition (EMT), and promoted apoptosis, whereas circ_0078767 knockdown resulted in the opposite effects. MicroRNA-889 (miR-889) was targeted and regulated by circ_0078767, and miR-889 could negatively modulate Kruppel-like factor 9 (KLF9) expression. Besides, circ_0078767 positively regulated KLF9 expression in OS cells via repressing miR-889. In conclusion, circ_0078767 enhances KLF9 expression by targeting miR-889 to inhibit OS progression.

show abstract

“…In addition, LINC0092 was considered a therapeutic marker for breast cancer and was regulated by hsa-miR-452-5p [26]. Hsa-miR-130b-3p [25], hsa-miR-29a-3p [27], hsa-miR-107 [32], hsa-miR-99b-5p [63], hsa-miR-99b-3p [64], hsa-miR-27b-5p [65], hsa-miR-125a-3p [66], hsa-miR-331-3p [67], hsa-miR-629-5p [68], hsa-miR-671-5p [69], hsa-miR-889-3p [70], and hsa-miR-1266-5p [71] have been proved to be highly related to breast cancer. These 12 miRNAs may be considered potential biomarkers for breast cancer, providing a new idea for related research.…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNA biomarkers for breast cancer by combining ensemble regularized multinomial logistic regression and Cox regression

Zhang

Gao

2022

BMC Bioinformatics

View full text Add to dashboard Cite

Background Breast cancer is one of the most common cancers in women. It is necessary to classify breast cancer subtypes because different subtypes need specific treatment. Identifying biomarkers and classifying breast cancer subtypes is essential for developing appropriate treatment methods for patients. MiRNAs can be easily detected in tumor biopsy and play an inhibitory or promoting role in breast cancer, which are considered promising biomarkers for distinguishing subtypes. Results A new method combing ensemble regularized multinomial logistic regression and Cox regression was proposed for identifying miRNA biomarkers in breast cancer. After adopting stratified sampling and bootstrap sampling, the most suitable sample subset for miRNA feature screening was determined via ensemble 100 regularized multinomial logistic regression models. 124 miRNAs that participated in the classification of at least 3 subtypes and appeared at least 50 times in 100 integrations were screened as features. 22 miRNAs from the proposed feature set were further identified as the biomarkers for breast cancer by using Cox regression based on survival analysis. The accuracy of 5 methods on the proposed feature set was significantly higher than on the other two feature sets. The results of 7 biological analyses illustrated the rationality of the identified biomarkers. Conclusions The screened features can better distinguish breast cancer subtypes. Notably, the genes and proteins related to the proposed 22 miRNAs were considered oncogenes or inhibitors of breast cancer. 9 of the 22 miRNAs have been proved to be markers of breast cancer. Therefore, our results can be considered in future related research.

show abstract

Tumour-suppressing functions of the lncRNA MBNL1-AS1/miR-889-3p/KLF9 axis in human breast cancer cells

Cited by 9 publications

References 31 publications

LncRNA MBNL1-AS1 Suppresses Cell Proliferation and Metastasis of Pancreatic Adenocarcinoma through Targeting Carcinogenic miR-301b-3p

LncRNA MBNL1-AS1 Suppresses Cell Proliferation and Metastasis of Pancreatic Adenocarcinoma through Targeting Carcinogenic miR-301b-3p

Circular RNA_0078767 upregulates Kruppel-like factor 9 expression by targeting microRNA-889, thereby inhibiting the progression of osteosarcoma

Identification of miRNA biomarkers for breast cancer by combining ensemble regularized multinomial logistic regression and Cox regression

Contact Info

Product

Resources

About