Tumour Production in Adult Mice by Syngeneic Embryonic Cultured Cells containing the Incomplete Rous Virus

Kryukova, I. N.; Obuch, I. B.; Biryulina, T. I.

doi:10.1038/219174a0

Cited by 8 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same is true for some of polyoma virus-transformed mouse cell lines [19,37], 5V40-transformed monkey embryo cells [22], RSV-transformed mouse embryo cells [11,12], and Adl-or Ad2-transformed rat embryo cells [16,3]. Nontransplantable SV40-and polyoma virus-transformed mouse cell lines, and Ad-transformed rat cells are said to be highly immunosensitive [19,27].…”

Section: Discussionmentioning

confidence: 99%

Alternate Changes of Surface Antigen(s) in Adenovirus Type 12‐Transformed and Tumor Cells1

Nakajima

Hamada

Uetake

1973

Japanese Journal of Microbiology

View full text Add to dashboard Cite

Alternate changes of specific surface antigen(s) (S antigen) were examined in transformed and tumor cells induced by human adenovirus type 12. All of the hamster and mouse cells transformed in vitro showed ring-form membrane fluorescence staining by anti-S antigen rabbit sera, whereas tumor cells, either induced by the virus in vivo or produced by inoculation with the S(+)-transformed cells, did not show any fluorescence. When the S(-) tumor cells were serially subcultured in vitro, all of them converted to S(+) cells, although more than ten subcultures were necessary. For the S(+) cells to form tumors in hamsters about ten times as many cells were necessary as the S( -) cells. This difference became greater when tumor formation was tested in preimmunized hamsters, while little, if any, when tested in X-irradiated hamsters. In addition, immunogenicity of the S(+) cells was suggested to be higher than that of the S(-) cells. These findings indicate that the S(+) cells are more immunosensitive and immunogenic than the S(-) cells, and that in vivo conversion from S(-1-) to S(-) may be due to selection of S(-)-mutant cells. In vitro conversion from S(-) to S(+) was also suggested to be due to the appearance of S (+) -mutant cells. [1,27,31,33] and transplantation rejection [5,24,26]. One of them which was demonstrable by immunofluorescence staining was designated as tumor-specific surface antigen (S antigen) and the other which was demonstrable by transplantation immunity is designated as tumor-specific transplantation antigen (TS-TA). However, the exact relationship between S antigen and TSTA is not clear yet.As pointed out in our preceding paper [6], we have noticed that S antigen was detectable by immunofluorescence staining on hamster and mouse cells transformed in vitro by human adenovirus type 12 and maintained by subcultures in vitro, while it was not detectable on tumor cells induced in vivo by the same virus. Since these observations were made with a small number of cell lines, extended and systematic experiments were carried out in order to determine 1) whether or not it is a general phenomenon, and, if general, 2) whether it is possible or not to convert S antigen-positive (S(+) ) cells to S antigen-negative (S( -) ) cells and vice versa, and when possible, 3) what the mechanism of the antigen conversion is.

show abstract

Section: Discussionmentioning

confidence: 99%

Alternate Changes of Surface Antigen(s) in Adenovirus Type 12‐Transformed and Tumor Cells1

Nakajima

Hamada

Uetake

1973

Japanese Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…This rat 'variant' of B77 was shown to be antigenically modified and had increased transforming activity on rat cells [Altaner and Temin, 1970] and hamster cells [Svec et al, 1970]. Kryukova et al [1968Kryukova et al [ ,1970, Obukh and Kryukova [1969], and found that tumors are efficiently produced in adult mice if fibroblasts are first infected in vitro with CZ-RSV and then transferred to syngeneic animals. From such tumors 'var iants' again were obtained which showed high oncogenicity for mice and hamsters.…”

Section: The Src Gene Product and Cell Transformationmentioning

confidence: 99%

Rous Sarcoma Virus

Svoboda¹

1986

Intervirology

View full text Add to dashboard Cite

Malignant and transforming activity of rous sarcoma virus. I. Malignant effect of rous sarcoma virus

Obukh

Kryukova

Biryulina

et al. 1969

Intl Journal of Cancer

View full text Add to dashboard Cite

Cultures of cells from embryos of Af, C3H-Hg and (CBAT6T6 x Af)F, mice rapidly developed oncogenicity for syngeneic animals after exposure to RSV (Carr-Zilber strain). The tumours of mice were virogenic and contained the group-specific antigen of avian sarcoma-leukosis complex. However, when the infected cells became transformed in morphology, they lost their oncogenicity for syngeneic animals.The high oncogenicity of Af mouse embryonic cells infected with Carr-Zilber strain of RSV for the syngeneic animals was described in our previous communication (Kryukova et al., 1968).The tumours were virogenic, i.e. the introduction of the intact cells into chicks produced Rous sarcomas. After transplantation of these virogenic tumours into chicks and passing through chicks the resulting virus could induce tumours in mice. The transformed cultures appeared to be non-oncogenic for syngeneic Af mice. The further investigation of this phenomenon is described in this paper. MATERIAL AND METHODS Preparation of virus (Carr-Zilber strain)Virus-containing chicken tumour extract, diluted 1 :3 and centrifuged at 400 x g for 20 min, was injected intramuscularly into 7to 10-day-old White Leghorn chicks. Five or 6 days after injection the tumours were excised, pooled, homogenized, frozen in a dry-iceacetone mixture, and then stored at -30" C.

show abstract

Tumour Production in Adult Mice by Syngeneic Embryonic Cultured Cells containing the Incomplete Rous Virus

Cited by 8 publications

References 1 publication

Alternate Changes of Surface Antigen(s) in Adenovirus Type 12‐Transformed and Tumor Cells1

Alternate Changes of Surface Antigen(s) in Adenovirus Type 12‐Transformed and Tumor Cells1

Rous Sarcoma Virus

Malignant and transforming activity of rous sarcoma virus. I. Malignant effect of rous sarcoma virus

Contact Info

Product

Resources

About