Tumour Markers in Peritoneal Washing Fluid - Contribution to Cytology

Yı́ldı́rı́m, Mustafa; Süren, Di̇nç; Yıldız, Mustafa; Ali̇kanoğlu, Arsenal Sezgi̇n; Kaya, Vi̇ldan; Doluoglu, Suleyman Gunhan; Aydın, Özgür; Yılmaz, Necat; Sezer, Cem; Karaca, Mehmet

doi:10.7314/apjcp.2013.14.2.1027

Cited by 3 publications

(5 citation statements)

References 16 publications

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“…Hence various ancillary techniques like immunocytochemistry, tumor markers, image morphometry, flow cytometry and others are being evaluated (Murugan et al, 2009;Krishan et al, 2010;Bunjhoo et al, 2012;Cheng et al, 2012;Kundu et al, 2012;Liu et al, 2013;Yildirim et al, 2013;Wang et al, 2013;2014). Computerised image analysis can estimate various parameters of the cells.…”

Section: 6919 Utility Of Nuclear Morphometry With Imagej In Effusionmentioning

confidence: 99%

“…Cytological diagnosis of effusion fluids is an important investigation to determine the appropriate treatment.The distinction of reactive mesothelial cells from carcinoma cells can sometimes prove to be a diagnostic challenge even for the experienced cytopathologist. Hence there is an increasing need to develop cost-effective ancillary methods to enhance the accuracy of cytologic diagnosis (Murugan et al, 2009;Bunjhoo et al, 2012;Cheng et al, 2012;Liu et al, 2013;Yildirim et al, 2013;Wang et al, 2013;2014). Image morphometry is one such ancillary technique and often requires expensive software and hardware for image acquisition and analysis.…”

Section: Introductionmentioning

confidence: 99%

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Utility of Nuclear Morphometry in Effusion Cytology

Ambroise

Jothilingam²,

Ramdas³

2014

Asian Pacific Journal of Cancer Prevention

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Background: The cytological analysis of serous effusions is a common investigation and yields important diagnostic information. However, the distinction of reactive mesothelial cells from malignant cells can sometimes be difficult for the cytopathologist. Hence cost-effective ancillary methods are essential to enhance the accuracy of cytological diagnosis. The aim of this study was to examine the utility of nuclear morphometry in differentiating reactive mesothelial cells from malignant cells in effusion smears. Materials and Methods: Sixty effusion smears consisting of 30 effusions cytologically classified as malignant (adenocarcinomas) and 30 benign effusions showing reactive mesothelial cells were included in the study. ImageJ was used to measure the nuclear area, perimeter, maximal feret diameter, minimal feret diameter and the circularity. A total of ten representative cells were studied in each case. Results: Significant differences were found between benign and malignant effusions for the nuclear area, perimeter, maximal feret diameter and minimal feret diameter. No significant difference was found for circularity, a shape descriptor. Receiver operating characteristic (ROC) curve analysis revealed that nuclear area, perimeter, maximal feret diameter, and minimal feret diameter are helpful in discriminating benign and malignant effusions. Conclusions: Computerised nuclear morphometry is a helpful ancillary technique to distinguish benign and malignant effusions. ImageJ is an excellent cost effective tool with potential diagnostic utility in effusion cytology.

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Section: 6919 Utility Of Nuclear Morphometry With Imagej In Effusionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Utility of Nuclear Morphometry in Effusion Cytology

Ambroise

Jothilingam²,

Ramdas³

2014

Asian Pacific Journal of Cancer Prevention

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“…The solution volume is the most important factor in determining the tumor marker concentration. We found very varied data on the volume in published studies, from 10 to 1000 ml [7, 9, 20, 21]. In order to elucidate the influence of solution volume on tumor marker concentrations, we compared the results acquired with two different volumes (20 in 50 ml), while the time and areas of washing were always the same.…”

Section: Discussionmentioning

confidence: 99%

“…Among factors that may contribute to the relatively high false-negative rate (20%) of cytology is infrequent exfoliation of malignant cells, but washing not being performed correctly can also be a factor [23, 24]. A SSP can thus make a contribution to cytology sampling, since an increased level of tumor marker(s) in peritoneal washing could still be detected [9]. Determination of tumor marker(s) concentration in washing may thus be useful in combination with cytology in order to obtain more accurate results, especially in classification of early-stage disease.…”

Section: Discussionmentioning

confidence: 99%

“…In early ovarian cancer, elevation of the concentration of tumor markers in the local environment may contribute to surgical staging, especially in cases in which PF is absent and a representative cytological sample is difficult to obtain [7–9]. Since peritoneal washing is used in such cases, knowing the relationship of tumor marker concentrations between PF and peritoneal washing is essential.…”

Section: Introductionmentioning

confidence: 99%

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Control values of ovarian cancer tumor markers and standardisation of a protocol for sampling peritoneal fluid and performing washing during laparoscopy

et al. 2014

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BackgroundDetermination of the tumor marker concentration in peritoneal fluid (PF) may help to assess its potential to detect small concentration changes between benign ovarian pathology and early stage ovarian cancer. Peritoneal washing, which can also be obtained when PF is absent, is already included in the International Federation of Gynecology and Obstetrics (FIGO) staging classification for ovarian cancer but sampling has not yet been standardized. Since our aim was to evaluate the relationship between marker concentration in PF and washing, standardization of the sampling protocol was a prerequisite to ensure reliable results.MethodsThirty-three women with non-malignant pathology of the reproductive organs were included in the study. We used three promising tumor markers for evaluation of the marker concentration in local fluid: osteopontin (sOPN), splice variant 6 of sCD44 (sCD44-v6) and vascular cell adhesion molecule-1 (sVCAM-1). After aspiration of PF, washing of the uterus, ovaries and pelvic peritoneum was performed with saline solution. Patients were divided into two groups based on the solution volume: A-20 ml and B-50 ml. To determine the efficiency of washing in relation to solution volume, washing was repeated three times. Concentrations of markers in samples were determined using flow cytometry.ResultsMean concentrations of markers were significantly higher (P <0.001) in PF than in the first washing. We demonstrated a significant positive correlation between marker concentrations in PF and first washing (sOPN: r = 0.447, P = 0.048; sCD44-v6: r = 0.660, P = 0.002; sVCAM-1: r = 0.526, P = 0.017). When using a smaller solution volume for washing, significantly higher (sVCAM-1: 2.5-fold, P = 0.021; sOPN: 3-fold, P = 0.024) or equal (sCD44-v6) mean concentrations of tumor markers were obtained.ConclusionsOur work demonstrates for the first time that concentrations of sOPN, sCD44-v6 and sVCAM-1 in PF correlate with peritoneal washing in women with non-malignant pathology of the reproductive organs. This indicates that, for selected tumor markers, washing can replace PF when PF is absent. A standardized protocol for sampling PF and performing washing during laparoscopy was established.

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